MSH2_SCHPO
ID MSH2_SCHPO Reviewed; 982 AA.
AC O74773; O42950;
DT 06-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1999, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=DNA mismatch repair protein msh2;
GN Name=msh2; ORFNames=SPBC19G7.01c, SPBC24C6.12c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9858548; DOI=10.1128/mcb.19.1.241;
RA Rudolph C., Kunz C., Parisi S., Lehmann E., Hartsuiker E., Fartmann B.,
RA Kramer W., Kohli J., Fleck O.;
RT "The msh2 gene of Schizosaccharomyces pombe is involved in mismatch repair,
RT mating-type switching, and meiotic chromosome organization.";
RL Mol. Cell. Biol. 19:241-250(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: Involved in post-replicative DNA-mismatch repair. Binds to
CC mismatch-containing DNA. Required for correct termination of copy
CC synthesis during mating-type switching. Also required for proper
CC chromosome organization during meiosis.
CC -!- SUBUNIT: Heterodimer of msh2 and msh6. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family.
CC {ECO:0000305}.
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DR EMBL; AJ006948; CAA07342.1; -; Genomic_DNA.
DR EMBL; CU329671; CAA21156.1; -; Genomic_DNA.
DR PIR; T43699; T43699.
DR RefSeq; XP_001713136.1; XM_001713084.2.
DR AlphaFoldDB; O74773; -.
DR SMR; O74773; -.
DR BioGRID; 277219; 45.
DR STRING; 4896.SPBC19G7.01c.1; -.
DR MaxQB; O74773; -.
DR PaxDb; O74773; -.
DR PRIDE; O74773; -.
DR EnsemblFungi; SPBC19G7.01c.1; SPBC19G7.01c.1:pep; SPBC19G7.01c.
DR PomBase; SPBC19G7.01c; msh2.
DR VEuPathDB; FungiDB:SPBC19G7.01c; -.
DR eggNOG; KOG0218; Eukaryota.
DR eggNOG; KOG0219; Eukaryota.
DR HOGENOM; CLU_002472_10_0_1; -.
DR InParanoid; O74773; -.
DR OMA; LFRIYQV; -.
DR PhylomeDB; O74773; -.
DR Reactome; R-SPO-5358565; Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
DR Reactome; R-SPO-5358606; Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta).
DR PRO; PR:O74773; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0032301; C:MutSalpha complex; IBA:GO_Central.
DR GO; GO:0000228; C:nuclear chromosome; ISO:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0005524; F:ATP binding; ISO:PomBase.
DR GO; GO:0016887; F:ATP hydrolysis activity; ISO:PomBase.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0000406; F:double-strand/single-strand DNA junction binding; ISO:PomBase.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0000400; F:four-way junction DNA binding; ISO:PomBase.
DR GO; GO:0030983; F:mismatched DNA binding; IBA:GO_Central.
DR GO; GO:0000403; F:Y-form DNA binding; ISO:PomBase.
DR GO; GO:0007534; P:gene conversion at mating-type locus; IMP:PomBase.
DR GO; GO:0043570; P:maintenance of DNA repeat elements; IMP:PomBase.
DR GO; GO:0006298; P:mismatch repair; IMP:PomBase.
DR GO; GO:0006312; P:mitotic recombination; IBA:GO_Central.
DR Gene3D; 3.30.420.110; -; 1.
DR Gene3D; 3.40.1170.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR011184; DNA_mismatch_repair_Msh2.
DR InterPro; IPR007695; DNA_mismatch_repair_MutS-lik_N.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007861; DNA_mismatch_repair_MutS_clamp.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR016151; DNA_mismatch_repair_MutS_N.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR007860; DNA_mmatch_repair_MutS_con_dom.
DR InterPro; IPR032642; Msh2.
DR InterPro; IPR036678; MutS_con_dom_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR PANTHER; PTHR11361:SF35; PTHR11361:SF35; 1.
DR Pfam; PF01624; MutS_I; 1.
DR Pfam; PF05188; MutS_II; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF05190; MutS_IV; 1.
DR Pfam; PF00488; MutS_V; 1.
DR PIRSF; PIRSF005813; MSH2; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Nucleotide-binding;
KW Nucleus; Reference proteome.
FT CHAIN 1..982
FT /note="DNA mismatch repair protein msh2"
FT /id="PRO_0000115190"
FT BINDING 721..728
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 982 AA; 109739 MW; 6A5F5AF711620244 CRC64;
MSSRNASIAN ERTDEARMFN FYEKMPKDTN TVRVFDRGEF YVAIGEDASF VAQNAYHTTS
VLKHHNVSNT SYCNLSPSLF IKFAEDVLSN LAKRVEIWGA NSAKTGFELL KQASPGNMQM
LEDLLVSENY QESTAISDSS VSSVLLAVTT RVKQDQRIIG VAFIDPILKK LGVSEFVDSD
AYTNFEALIV QVGAKECIIS QSGHESTNGN SAVSINTAEI NRLRNIIEGC GALVTTIRSS
EFSARDVELE LSKVLDSPVT HALVPELGLQ LAMASCNALL RYLGPALLNP DMEDDRENTS
RKLHLYHHNL EQYMRLDIAA VRSLNLLPPP NGNAHKTMSL YGLLNHCRTA MGARNLRRWI
VQPLLDAKSI ERRHDLVSAL VEDAEARQLL LDDDHLLRSI PDIPKLCRRL TRGSASLEDV
VRIYQMAKAL PKIVTVLDSL TSEHKDLVDK VYTNVLNNHC KNLEKLIELV ETTIDLEALD
SHQYIIRAEF DEELLDLRQR LDELQHSIFE EHKRVGSDLH QDTEKKLHLE QHHLYGWCLR
LTRTEAGCLR GRSSHYTELS TQKNGVYFTT KRLHSLNNSY MDHQKSYRYH QNGLAREVIK
IAATYGPPLE AIGQVIAHLD VILSFAHAST VAVIPYVRPN IVDSSIAQEK HGQSSNILDI
VSLEDTPNFE EIRRTLENNH CARLYLKQAR HPCLEAQDDV KFIPNDVNLE HGSSELLIIT
GPNMGGKSTY IRQVGVITVM AQIGCPVPCE VADLDIIDAI LARVGASDSQ LKGISTFMAE
MLETATILRA ATPRSLIIID ELGRGTSTTD GFGLAWAITE HIVTQIGCFC LFATHYHEMT
KLSEEITTVK NLHVTAYVGD SESKDVALLY NVCEGASDRS FGIHVAKLAH FPPKIIEMAS
NKAAELEAED SGAQGDTQEV KSKKEGMAIV RDIMRQWRSN VKPEMTQQQM MDQFQKVIGS
FAQQINSNNW LQSRVSTNEV SS
