MSH3_DICDI
ID MSH3_DICDI Reviewed; 1428 AA.
AC Q1ZXH0;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 02-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=DNA mismatch repair protein Msh3;
DE AltName: Full=MutS protein homolog 3;
GN Name=msh3; ORFNames=DDB_G0281683;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Component of the post-replicative DNA mismatch repair system
CC (MMR). Heterodimerizes with msh2 to form MutS beta, which binds to DNA
CC mismatches thereby initiating DNA repair. When bound, the MutS beta
CC heterodimer bends the DNA helix and shields approximately 20 base
CC pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to
CC 13 nucleotides long. After mismatch binding, forms a ternary complex
CC with the MutL alpha heterodimer, which is thought to be responsible for
CC directing the downstream MMR events, including strand discrimination,
CC excision, and resynthesis (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer consisting of msh2-msh3 (MutS beta). Forms a
CC ternary complex with MutL alpha (mlh1-pms1). Interacts with exo1 (By
CC similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MSH3
CC subfamily. {ECO:0000305}.
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DR EMBL; AAFI02000042; EAS66875.1; -; Genomic_DNA.
DR RefSeq; XP_001134558.1; XM_001134558.1.
DR AlphaFoldDB; Q1ZXH0; -.
DR SMR; Q1ZXH0; -.
DR STRING; 44689.DDB0232960; -.
DR PaxDb; Q1ZXH0; -.
DR EnsemblProtists; EAS66875; EAS66875; DDB_G0281683.
DR GeneID; 8623184; -.
DR KEGG; ddi:DDB_G0281683; -.
DR dictyBase; DDB_G0281683; msh3.
DR eggNOG; KOG0218; Eukaryota.
DR HOGENOM; CLU_002472_0_2_1; -.
DR InParanoid; Q1ZXH0; -.
DR OMA; LRNVHMK; -.
DR PhylomeDB; Q1ZXH0; -.
DR Reactome; R-DDI-5358606; Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta).
DR PRO; PR:Q1ZXH0; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0032302; C:MutSbeta complex; ISS:dictyBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0032135; F:DNA insertion or deletion binding; ISS:dictyBase.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0006310; P:DNA recombination; ISS:dictyBase.
DR GO; GO:0006298; P:mismatch repair; ISS:dictyBase.
DR GO; GO:0006312; P:mitotic recombination; ISS:dictyBase.
DR GO; GO:0000735; P:removal of nonhomologous ends; ISS:dictyBase.
DR Gene3D; 3.30.420.110; -; 1.
DR Gene3D; 3.40.1170.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR007695; DNA_mismatch_repair_MutS-lik_N.
DR InterPro; IPR017261; DNA_mismatch_repair_MutS/MSH.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR016151; DNA_mismatch_repair_MutS_N.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR007860; DNA_mmatch_repair_MutS_con_dom.
DR InterPro; IPR036678; MutS_con_dom_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR Pfam; PF01624; MutS_I; 1.
DR Pfam; PF05188; MutS_II; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF00488; MutS_V; 1.
DR PIRSF; PIRSF037677; DNA_mis_repair_Msh6; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF53150; SSF53150; 1.
DR SUPFAM; SSF55271; SSF55271; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..1428
FT /note="DNA mismatch repair protein Msh3"
FT /id="PRO_0000328253"
FT REGION 1..391
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 405..451
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 592..612
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..22
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 36..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 51..98
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 99..127
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 128..167
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 188..205
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 217..258
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 259..287
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 288..307
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 424..440
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 592..607
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1428 AA; 161570 MW; 9634B4FBA4E20C8C CRC64;
MPRLKLPKSF KDELESEKTT TTSSRKKAPV VDPKQTLMSS FFTPVSKSTD TKEINNKEDK
DEDKDKDKDN KKTKKSKDTS DNEDMVDDNN KQQKEKKATP NKSNSPQSPQ TSKSPLTRRS
SANGNSDSKI DNKEKEKEKE KEKEKDKSTP TKTTSSRVKK DTEVSKSIPP KLSKQTKKKQ
ISSDNDIYDD EKDSEEEDLE DDHDDEEEKV VVKKPSKPTS KSITAKPAST KATTTTTTTT
TTTSTGRTRV NRVNLDISFS SESEEDEKPK KKIIGKKRKK KDDSDFDSES ESDTISEASE
VEPESEEDLD SFKFNSKKKN NNKNNNNKKK NDEYEDEEED EDDELFKDIE MKDKPEEEEK
EEEGDPIVIG SGRVVLPKGT PDFQPDPKAG KKLLKAHLEI QSKEEAKRLQ QANGGGGDGG
GGQIKGSDDE DEEVKKPTKG GSKASAKKKG PAYTPLEQQY IAIKKENPDT VLMVECGYKY
KFFGEDAEVA NKVLNIYSYV AKNFLNCSIP TQRLFFHLRR LVMAGYKVGI VEQTETAALK
AISSSKSQPF ERKLTRVYTS STFIDDDIDD QLTSSSPQFL VSFYESTPKN KNDDVIKKQR
DNEEEGIDSS NESSTSTISF VAVSVKTGEI IYDTFKDNVM RSQLETILTH IKPSEILIPP
TTTTVNKQKV NNGIGTNHYY FSNLTSKCLK TYTKSTNVRT QAMDSQLYDY EYSLGKLIDF
YEDESNNNNN NNNCEDVLKF VKSTLNKEQI ICLGILLSYL NEFIHFGSIL KVESNFKAFR
VSNHLVLPHS TIVNLELLVN ESDNKEKGSL IWLMNRTSTF SGSRMFINWI CKPLNQLELI
KERQDAVEEL VNGIKTNSPP IVSIISLFKS HIPDLQRNLS RIYYKVQCTP KEFLNTMTSL
QRIVELFKEI NNNNSSYKFN STLLNSIFKL QNDNKDGDSD SFDYIGGEDK LSKRIKYFLS
NINKETAKEY GTVGCDKSNL WVDLEKYEKI RETKEKIEQV EKEFKNVLKN IRKELSKPSL
EYHHMPGLGL EYLLELPPSF KAVPKSWIKV NSTQKMARYH APEVLEQLKI LSQSRETLKI
QSQESWISFL GEFSVDYSLF SNFVNKISNL DCLFSLAKVS SLEGYIRPQF VKEKKDGGIQ
IENGRHPVVE AILSGSDGSY VPNTIELRES ACKSMIITGP NMGGKSSLLR QTALIVIMAQ
VGCFVPATSC SLSVFDAIYT RMGARDSIGT GKSTFFIELE ETSDILKNST QNTLVILDEL
GRGTSTNDGV AIAYSTLKYI VEVMKCYCLF VTHYPLLAQL ELQYPTQVGN FHMGYLEEKQ
DQQLQKSVIP KVIFLYKLVK GAAQNSYGLN IARLAGLPME VIADALKKSN EMKESITRRA
NLSDGKDQQQ IENEIKSIIK NWNSNRTTLN SNDLLQFIEK FKSIQLKL
