MSH3_PICGU
ID MSH3_PICGU Reviewed; 960 AA.
AC A5DEV6;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 2.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=DNA mismatch repair protein MSH3;
DE AltName: Full=MutS protein homolog 3;
GN Name=MSH3; ORFNames=PGUG_01807;
OS Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539
OS / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Meyerozyma.
OX NCBI_TaxID=294746;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324;
RX PubMed=19465905; DOI=10.1038/nature08064;
RA Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S.,
RA Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I.,
RA Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C.,
RA Fitzpatrick D.A., de Groot P.W.J., Harris D., Hoyer L.L., Hube B.,
RA Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M.,
RA Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F.,
RA Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D.,
RA Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T.,
RA Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C.,
RA Birren B.W., Kellis M., Cuomo C.A.;
RT "Evolution of pathogenicity and sexual reproduction in eight Candida
RT genomes.";
RL Nature 459:657-662(2009).
CC -!- FUNCTION: Component of the post-replicative DNA mismatch repair system
CC (MMR). Heterodimerizes with MSH2 to form MutS beta, which binds to DNA
CC mismatches thereby initiating DNA repair. MSH3 provides substrate-
CC binding and substrate specificity to the complex. When bound, the MutS
CC beta heterodimer bends the DNA helix and shields approximately 20 base
CC pairs. Acts mainly to repair insertion-deletion loops (IDLs) from 2 to
CC 13 nucleotides in size, but can also repair base-base and single
CC insertion-deletion mismatches that occur during replication. After
CC mismatch binding, forms a ternary complex with the MutL alpha
CC heterodimer, which is thought to be responsible for directing the
CC downstream MMR events, including strand discrimination, excision, and
CC resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch
CC repair functions (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer consisting of MSH2-MSH3 (MutS beta). Forms a
CC ternary complex with MutL alpha (MLH1-PMS1) (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MSH3
CC subfamily. {ECO:0000305}.
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DR EMBL; CH408156; EDK37709.2; -; Genomic_DNA.
DR RefSeq; XP_001486136.1; XM_001486086.1.
DR AlphaFoldDB; A5DEV6; -.
DR SMR; A5DEV6; -.
DR STRING; 4929.XP_001486136.1; -.
DR EnsemblFungi; EDK37709; EDK37709; PGUG_01807.
DR GeneID; 5127810; -.
DR KEGG; pgu:PGUG_01807; -.
DR eggNOG; KOG0218; Eukaryota.
DR HOGENOM; CLU_002472_0_2_1; -.
DR InParanoid; A5DEV6; -.
DR OMA; LRNVHMK; -.
DR OrthoDB; 138168at2759; -.
DR Proteomes; UP000001997; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR Gene3D; 3.30.420.110; -; 1.
DR Gene3D; 3.40.1170.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR007695; DNA_mismatch_repair_MutS-lik_N.
DR InterPro; IPR017261; DNA_mismatch_repair_MutS/MSH.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR016151; DNA_mismatch_repair_MutS_N.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR036678; MutS_con_dom_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR Pfam; PF01624; MutS_I; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF00488; MutS_V; 1.
DR PIRSF; PIRSF037677; DNA_mis_repair_Msh6; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF53150; SSF53150; 1.
DR SUPFAM; SSF55271; SSF55271; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Nucleotide-binding;
KW Nucleus; Reference proteome.
FT CHAIN 1..960
FT /note="DNA mismatch repair protein MSH3"
FT /id="PRO_0000338528"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 53..93
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 87..209
FT /note="Mispair-binding domain"
FT /evidence="ECO:0000250"
FT BINDING 734..741
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 960 AA; 107853 MW; BEBEC1746CB4720D CRC64;
MKRGIDIVQA LKNSKRSKSS DDSVESKLSK ASPDLSQYAA VQISKEDVTV NPEVKSTIDN
HKKEPPSIAS SIKKSPKTVI GKHANSTKKP KKRTPLDQQF IDLKNEYPDC VLAIQVGYKY
KFFGVDAVPV SRILNIMFIP GNLTERDATH DKFAYCSVPD NRLHIHLQRL LTNGLKVAVV
SQTESAVLRE AESSKGLFLR EVTAIYTKAT YIEEGSGDFI SCITWNGTSA GAVTVQPCTG
EIIVEDFSEK EPEALSELQT YLHHLRPSEI IVTDNEATKE NDKLSRLLKS FGGARISYKP
FDEVSPIEEL LPASIANYYV TNHSTTTTQC ISQLITYLKD FSLDQIFTVP SNVSKFSTKM
YMNLPGNTLK ALEIFQNSSG TEKGTLFWHL DHTHTKMGRR MLQKWVSKPL IDNASIQERL
DAIESLMNYN YAVEVFEGII KKIGRDESDW EKSMIKIHYT ANGSQNRVTR KEVVQLLLQF
RSVIDTVYKF KSSFKDSSIS KLLQSMFSEL AELASTPIVN DLLSRVKIEA VYREEVEDQK
KEFFDLDSHP HEGIKSELNA ISELEQALQD ELVEIKKIIK KPVDYMTVSR EPYLIALRGD
SGPQDWLKIS ATKTVTRYRP PKVSKLYKEL LYHQEKLIQQ CDEAFATFLK DIDSHYTYFS
RLIKIVAEID CLLSLKATSS SNSGYSKPIL SDKQMIKAKR SRNPVIENLT STSQYVANDI
EISYDENRVL IITGPNMGGK SSYVKQVALI ALMTQIGCYL PCESAIVGIF DTILVRMGAE
DNILKGESTF MVEMSECSTI IKSLTNKSLV ILDEIGRGTG TEDGIAIAYS IISYLIEEPR
LPLTLFITHY PSLKVLEDTH PKNVANYHMG FMEVAKADQE WPDVTFLYTL KRGVVSNSYG
LNVARLAGIP SEIITKAFKV AEALKQDIED SELSSMGQIL KSEQSSASKL VEIDRIVSYI
