MSH3_PICST
ID MSH3_PICST Reviewed; 1025 AA.
AC A3LU10;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 3.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=DNA mismatch repair protein MSH3;
DE AltName: Full=MutS protein homolog 3;
GN Name=MSH3; ORFNames=PICST_59113;
OS Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS Y-11545) (Yeast) (Pichia stipitis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX NCBI_TaxID=322104;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545;
RX PubMed=17334359; DOI=10.1038/nbt1290;
RA Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA Passoth V., Richardson P.M.;
RT "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT yeast Pichia stipitis.";
RL Nat. Biotechnol. 25:319-326(2007).
CC -!- FUNCTION: Component of the post-replicative DNA mismatch repair system
CC (MMR). Heterodimerizes with MSH2 to form MutS beta, which binds to DNA
CC mismatches thereby initiating DNA repair. MSH3 provides substrate-
CC binding and substrate specificity to the complex. When bound, the MutS
CC beta heterodimer bends the DNA helix and shields approximately 20 base
CC pairs. Acts mainly to repair insertion-deletion loops (IDLs) from 2 to
CC 13 nucleotides in size, but can also repair base-base and single
CC insertion-deletion mismatches that occur during replication. After
CC mismatch binding, forms a ternary complex with the MutL alpha
CC heterodimer, which is thought to be responsible for directing the
CC downstream MMR events, including strand discrimination, excision, and
CC resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch
CC repair functions (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer consisting of MSH2-MSH3 (MutS beta). Forms a
CC ternary complex with MutL alpha (MLH1-PMS1) (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MSH3
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABN66162.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP000498; ABN66162.2; ALT_INIT; Genomic_DNA.
DR RefSeq; XP_001384191.2; XM_001384154.1.
DR AlphaFoldDB; A3LU10; -.
DR SMR; A3LU10; -.
DR STRING; 4924.XP_001384191.2; -.
DR PRIDE; A3LU10; -.
DR EnsemblFungi; ABN66162; ABN66162; PICST_59113.
DR GeneID; 4838728; -.
DR KEGG; pic:PICST_59113; -.
DR eggNOG; KOG0218; Eukaryota.
DR HOGENOM; CLU_002472_0_0_1; -.
DR InParanoid; A3LU10; -.
DR OrthoDB; 138168at2759; -.
DR Proteomes; UP000002258; Chromosome 4.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR Gene3D; 3.30.420.110; -; 1.
DR Gene3D; 3.40.1170.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR007695; DNA_mismatch_repair_MutS-lik_N.
DR InterPro; IPR017261; DNA_mismatch_repair_MutS/MSH.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR016151; DNA_mismatch_repair_MutS_N.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR007860; DNA_mmatch_repair_MutS_con_dom.
DR InterPro; IPR036678; MutS_con_dom_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR Pfam; PF01624; MutS_I; 1.
DR Pfam; PF05188; MutS_II; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF00488; MutS_V; 1.
DR PIRSF; PIRSF037677; DNA_mis_repair_Msh6; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF55271; SSF55271; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Nucleotide-binding;
KW Nucleus; Reference proteome.
FT CHAIN 1..1025
FT /note="DNA mismatch repair protein MSH3"
FT /id="PRO_0000338529"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 114..235
FT /note="Mispair-binding domain"
FT /evidence="ECO:0000250"
FT COMPBIAS 1..27
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 792..799
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1025 AA; 117247 MW; 40D6FDAC3D958A94 CRC64;
MSYRSKQASI SRFFKSTKSN NSAKNEPAVQ HIPKKTGVML KFSYNNKENV VEGGKDTEGP
HIVGSHVELP GQSVNSVVNS DSIVVNSNLE IDPKISSVLK RKPDIDIQLK TTKKRSKTLT
PLEKQIRELR ESHKDKVLVI QIGYKYKMFG DDAKLGSKIL DIMYIRGGDD GTRDEFSYCS
FPDFKLHINL KRLLTHGLKI GVVKQLESAI VKTVEKSSKS SDLMKREITG VYTRGTYMGD
EYVQSSGNSA DTESPYYIIC INEINQKELS MVAVQPKTGD IVQDTFKDGL NRDELETRLM
YLNPSEVIVL SSEQPSVETL KTIRLVASDV QLLPRKRKGE DEVFNGLIEF FDSIDNGKYK
HLGDYFSVNF SKHIQSCFYE LINYLSEFKL SNVFTIPDNI SNFTNSRKYM VLPNNTLYAL
EIFQNYTNPA SQKGTLIWLL NHTRTRFGNR LLNKWVSKPL IEKEKIEERL LAIEDLTGDF
NNVVDALKIQ LDKMGKSLDL EELLMKTHYA ATYNLDKINR RDIYNMLDCF QSVLESMNRF
EKGITEFSKT KKSPLLTNIL LELSEMSKTT VVSNLLNKIN RSYVMNESKD PEEQVTQFFN
LDNHNWEDIR SEFSELDKIE KLFEEELLNI RRVLKRPQLQ YITNNKEPYL IEVRNGKQVD
ELPTDFHRIN GTTTVSRFRS ERTAQLYIKK QYHKEKLLVN CNVAFNDFLK EIDEQYEFFS
KIVKNLSVFD CLLSLTAASL ASKNTRPILV DQQLIEVQKG RNPIIESLHN RNDYVPNDID
ICYDNKVLII TGPNMGGKSS YVKQVALLVI MSQIGCYIPC DRATLGVFDS IFIRMGASDN
ILKGNSTFMN EMLECSNIIH GISNKSLVIL DEIGRGTGTS DGIALAYSIL RYLIESPLRP
LVLFITHYPS LHVLEDSFPT VVTNYHMGFQ QIHKDDNDFP EIIFLYNLVK GVINNSYGLN
VAKLAGLPVS VISGAHRVSE SLKYKVEIQQ KEQFTMKFGS ILQMLKKDEI NSNNILELEN
LFSYI
