MSH4_ARATH
ID MSH4_ARATH Reviewed; 792 AA.
AC F4JP48; O23581; Q6DTM5;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=DNA mismatch repair protein MSH4;
DE Short=AtMSH4;
DE AltName: Full=MutS protein homolog 4;
GN Name=MSH4; OrderedLocusNames=At4g17380; ORFNames=dl4725w, FCAALL.423;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=15489296; DOI=10.1101/gad.317504;
RA Higgins J.D., Armstrong S.J., Franklin F.C.H., Jones G.H.;
RT "The Arabidopsis MutS homolog AtMSH4 functions at an early step in
RT recombination: evidence for two classes of recombination in Arabidopsis.";
RL Genes Dev. 18:2557-2570(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP FUNCTION.
RX PubMed=17530928; DOI=10.1371/journal.pgen.0030083;
RA Chelysheva L., Gendrot G., Vezon D., Doutriaux M.P., Mercier R., Grelon M.;
RT "Zip4/Spo22 is required for class I CO formation but not for synapsis
RT completion in Arabidopsis thaliana.";
RL PLoS Genet. 3:E83-E83(2007).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=18318687; DOI=10.1111/j.1365-313x.2008.03470.x;
RA Higgins J.D., Vignard J., Mercier R., Pugh A.G., Franklin F.C., Jones G.H.;
RT "AtMSH5 partners AtMSH4 in the class I meiotic crossover pathway in
RT Arabidopsis thaliana, but is not required for synapsis.";
RL Plant J. 55:28-39(2008).
RN [7]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=19153602; DOI=10.1038/emboj.2008.295;
RA Osman K., Sanchez-Moran E., Mann S.C., Jones G.H., Franklin F.C.;
RT "Replication protein A (AtRPA1a) is required for class I crossover
RT formation but is dispensable for meiotic DNA break repair.";
RL EMBO J. 28:394-404(2009).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=21217641; DOI=10.1038/emboj.2010.344;
RA Chen Z., Higgins J.D., Hui J.T., Li J., Franklin F.C., Berger F.;
RT "Retinoblastoma protein is essential for early meiotic events in
RT Arabidopsis.";
RL EMBO J. 30:744-755(2011).
CC -!- FUNCTION: Involved in meiotic recombination in association with MSH5.
CC Required for reciprocal recombination and proper segregation of
CC homologous chromosomes at meiosis. Promotes homologous recombination
CC through facilitating chiasma formation during prophase I. Involved in
CC the control of class I crossovers formation.
CC {ECO:0000269|PubMed:15489296, ECO:0000269|PubMed:17530928,
CC ECO:0000269|PubMed:19153602}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15489296,
CC ECO:0000269|PubMed:18318687, ECO:0000269|PubMed:19153602,
CC ECO:0000269|PubMed:21217641}. Note=In pollen mother cells during
CC meiosis, localizes to unsynapsed axes during leptotene and zygotene,
CC but is not present on synapsed regions of zygotene nuclei.
CC -!- TISSUE SPECIFICITY: Specifically expressed in flowers.
CC {ECO:0000269|PubMed:15489296}.
CC -!- DISRUPTION PHENOTYPE: Normal vegetative growth but severe reduction in
CC fertility due to a decrease in chiasma frequency at metaphase I of
CC meiosis. {ECO:0000269|PubMed:15489296}.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB10519.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78741.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY646927; AAT70180.1; -; mRNA.
DR EMBL; Z97343; CAB10519.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161546; CAB78741.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE83882.1; -; Genomic_DNA.
DR PIR; B71443; B71443.
DR RefSeq; NP_193469.2; NM_117842.3.
DR AlphaFoldDB; F4JP48; -.
DR SMR; F4JP48; -.
DR IntAct; F4JP48; 1.
DR STRING; 3702.AT4G17380.1; -.
DR PaxDb; F4JP48; -.
DR PRIDE; F4JP48; -.
DR EnsemblPlants; AT4G17380.1; AT4G17380.1; AT4G17380.
DR GeneID; 827450; -.
DR Gramene; AT4G17380.1; AT4G17380.1; AT4G17380.
DR KEGG; ath:AT4G17380; -.
DR Araport; AT4G17380; -.
DR TAIR; locus:2130913; AT4G17380.
DR eggNOG; KOG0220; Eukaryota.
DR HOGENOM; CLU_002472_7_3_1; -.
DR InParanoid; F4JP48; -.
DR OMA; AIAMLDM; -.
DR OrthoDB; 138168at2759; -.
DR PRO; PR:F4JP48; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; F4JP48; baseline and differential.
DR Genevisible; F4JP48; AT.
DR GO; GO:0000794; C:condensed nuclear chromosome; IDA:TAIR.
DR GO; GO:0043073; C:germ cell nucleus; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR GO; GO:0007129; P:homologous chromosome pairing at meiosis; IMP:TAIR.
DR GO; GO:0045143; P:homologous chromosome segregation; IMP:UniProtKB.
DR GO; GO:0010777; P:meiotic mismatch repair involved in reciprocal meiotic recombination; IMP:UniProtKB.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IBA:GO_Central.
DR Gene3D; 3.30.420.110; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR011184; DNA_mismatch_repair_Msh2.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007861; DNA_mismatch_repair_MutS_clamp.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR036678; MutS_con_dom_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF05190; MutS_IV; 1.
DR Pfam; PF00488; MutS_V; 1.
DR PIRSF; PIRSF005813; MSH2; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; DNA-binding; Meiosis; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..792
FT /note="DNA mismatch repair protein MSH4"
FT /id="PRO_0000418367"
FT BINDING 553..560
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 254
FT /note="K -> E (in Ref. 1; AAT70180)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 792 AA; 89015 MW; E1DCE70401C57A65 CRC64;
MEDDGGERSS FVAGLIENRA KEVGMAAFDL RSASLHLSQY IETSSSYQNT KTLLRFYDPS
VIIVPPNKLA ADGMVGVSEL VDRCYSTVRK VVFARGCFDD TKGAVLIQNL AAEEPLALGL
DTYYKQHYLS LAAAAATIKW IEAEKGVIVT NHSLTVTFNG SFDHMNIDAT SVENLELIDP
FHNALLGTSN KKRSLFQMFK TTKTAGGTRL LRANLLQPLK DIETINTRLD CLDELMSNEQ
LFFGLSQVLR KFPKETDRVL CHFCFKPKKV TEAVIGFENT RKSQNMISSI ILLKTALDAL
PILAKVLKDA KCFLLANVYK SVCENDRYAS IRKKIGEVID DDVLHARVPF VARTQQCFAL
KAGIDGFLDI ARRTFCDTSE AIHNLASKYR EEFNLPNLKL PFNNRQGFFF RIPQKEVQGK
LPNKFTQVVK HGKNIHCSSL ELASLNVRNK SAAGECFIRT ETCLEALMDA IREDISALTL
LAEVLCLLDM IVNSFAHTIS TKPVDRYSRP ELTDSGPLAI DAGRHPILES IHNDFVSNSI
FMSEATNMLV VMGPNMSGKS TYLQQVCLVV ILAQIGCYVP ARFATIRVVD RIFTRMGTMD
NLESNSSTFM TEMRETAFIM QNVTNRSLIV MDELGRATSS SDGLAMAWSC CEYLLSLKAY
TVFATHMDSL AELATIYPNV KVLHFYVDIR DNRLDFKFQL RDGTLHVPHY GLLLAEVAGL
PSTVIDTARI ITKRITDKEN KRIELNCGKH HEIHRIYRVA QRLICLKYSR QTEDSIRQAL
QNLNESFTEE RL
