MSH4_MOUSE
ID MSH4_MOUSE Reviewed; 958 AA.
AC Q99MT2; Q920J1;
DT 09-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=MutS protein homolog 4;
DE Short=mMsh4;
GN Name=Msh4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ; TISSUE=Testis;
RX PubMed=11178747; DOI=10.1007/s003350010231;
RA Her C., Wu X., Bailey S.M., Doggett N.A.;
RT "Mouse MutS homolog 4 is predominantly expressed in testis and interacts
RT with MutS homolog 5.";
RL Mamm. Genome 12:73-76(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 48-958.
RX PubMed=11553915; DOI=10.1002/mrd.1074;
RA Santucci-Darmanin S., Vidal F., Scimeca J.C., Turc-Carel C.,
RA Paquis-Flucklinger V.;
RT "Family of SRY/Sox proteins is involved in the regulation of the mouse Msh4
RT (MutS Homolog 4) gene expression.";
RL Mol. Reprod. Dev. 60:172-180(2001).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=30272023; DOI=10.1038/s42003-018-0154-z;
RA Zhang Q., Shao J., Fan H.Y., Yu C.;
RT "Evolutionarily-conserved MZIP2 is essential for crossover formation in
RT mammalian meiosis.";
RL Commun. Biol. 1:147-147(2018).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=30746471; DOI=10.1126/sciadv.aau9780;
RA Zhang Q., Ji S.Y., Busayavalasa K., Yu C.;
RT "SPO16 binds SHOC1 to promote homologous recombination and crossing-over in
RT meiotic prophase I.";
RL Sci. Adv. 5:eaau9780-eaau9780(2019).
CC -!- FUNCTION: Involved in meiotic recombination. Required for reciprocal
CC recombination and proper segregation of homologous chromosomes at
CC meiosis.
CC -!- SUBUNIT: Heterooligomer of MSH4 and MSH5.
CC -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000269|PubMed:30272023,
CC ECO:0000269|PubMed:30746471}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in testis.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family.
CC {ECO:0000305}.
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DR EMBL; AF298655; AAK15620.1; -; mRNA.
DR EMBL; AF178957; AAL18350.1; -; mRNA.
DR CCDS; CCDS38678.1; -.
DR RefSeq; NP_114076.1; NM_031870.3.
DR AlphaFoldDB; Q99MT2; -.
DR SMR; Q99MT2; -.
DR CORUM; Q99MT2; -.
DR STRING; 10090.ENSMUSP00000005630; -.
DR iPTMnet; Q99MT2; -.
DR PhosphoSitePlus; Q99MT2; -.
DR jPOST; Q99MT2; -.
DR PaxDb; Q99MT2; -.
DR PRIDE; Q99MT2; -.
DR ProteomicsDB; 290099; -.
DR Antibodypedia; 33475; 155 antibodies from 20 providers.
DR Ensembl; ENSMUST00000005630; ENSMUSP00000005630; ENSMUSG00000005493.
DR GeneID; 55993; -.
DR KEGG; mmu:55993; -.
DR UCSC; uc008rue.2; mouse.
DR CTD; 4438; -.
DR MGI; MGI:1860077; Msh4.
DR VEuPathDB; HostDB:ENSMUSG00000005493; -.
DR eggNOG; KOG0220; Eukaryota.
DR GeneTree; ENSGT00550000074897; -.
DR InParanoid; Q99MT2; -.
DR OMA; AIAMLDM; -.
DR OrthoDB; 138168at2759; -.
DR PhylomeDB; Q99MT2; -.
DR TreeFam; TF300572; -.
DR BioGRID-ORCS; 55993; 2 hits in 109 CRISPR screens.
DR ChiTaRS; Msh4; mouse.
DR PRO; PR:Q99MT2; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q99MT2; protein.
DR Bgee; ENSMUSG00000005493; Expressed in secondary oocyte and 11 other tissues.
DR ExpressionAtlas; Q99MT2; baseline and differential.
DR Genevisible; Q99MT2; MM.
DR GO; GO:0005694; C:chromosome; IDA:UniProtKB.
DR GO; GO:0000793; C:condensed chromosome; IDA:MGI.
DR GO; GO:0000794; C:condensed nuclear chromosome; IDA:MGI.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0005713; C:recombination nodule; IDA:MGI.
DR GO; GO:0000795; C:synaptonemal complex; IDA:MGI.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR GO; GO:0007292; P:female gamete generation; IMP:MGI.
DR GO; GO:0007129; P:homologous chromosome pairing at meiosis; IMP:MGI.
DR GO; GO:0051321; P:meiotic cell cycle; IDA:MGI.
DR GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR GO; GO:0001541; P:ovarian follicle development; IMP:MGI.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IBA:GO_Central.
DR GO; GO:0007283; P:spermatogenesis; IMP:MGI.
DR Gene3D; 3.30.420.110; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007861; DNA_mismatch_repair_MutS_clamp.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR007860; DNA_mmatch_repair_MutS_con_dom.
DR InterPro; IPR036678; MutS_con_dom_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR Pfam; PF05188; MutS_II; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF05190; MutS_IV; 1.
DR Pfam; PF00488; MutS_V; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF53150; SSF53150; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chromosome; DNA-binding; Meiosis; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..958
FT /note="MutS protein homolog 4"
FT /id="PRO_0000115198"
FT REGION 51..110
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 124..161
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 76..109
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 702..709
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 73..90
FT /note="RTLASASRPSTEGAMADR -> GPWPRVQAEYGRRHGRQ (in Ref. 2;
FT AAL18350)"
FT /evidence="ECO:0000305"
FT CONFLICT 125
FT /note="V -> I (in Ref. 2; AAL18350)"
FT /evidence="ECO:0000305"
FT CONFLICT 372..373
FT /note="RL -> TV (in Ref. 2; AAL18350)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 958 AA; 107006 MW; 936962E370C4B240 CRC64;
MCCLFLRLRD YSTAHALSLP PCQRCGLQPW SARSHARRTL GVRKAGEMLR QEAASLSSSP
RWTPSRRDAP CGRTLASASR PSTEGAMADR SSSSSSSPAP ASAPGSSFGN KRSYAIHRAA
SSFPVGTSSS SARDTTYPHT FRTPLSAGNP QRSGHKSWTP QVGYSATSSA VSAHAPSVIV
AVVEGRGLAR GEIGMASIDL KSPQIMLSQF ADNTTYAKVI TKLQVLSPLE IIMSNTACVV
GNSTKLFTLI TENFKNVNFT TVQRKYFNET KGLEYIEQLC IAEFSSVLME VQSRYYCLAA
AAALLKYVEF IQNSVYAPKS LKIYFQGSEQ TAMIDSSSAQ NLELLVNNQD YRSNHTLFGV
LNYTKTAGGS RRLRSNILEP LVDVETISMR LDCVQELLQD EELFFGLQSV ISRFLDTEQL
LSVLVQIPKQ DTVNAAESKI TNLIYLKHTL ELVEPLKVTL KNCSTPLLRA YYGSLEDHRF
GLILDKIKTV INDDARYMKG CLNMRTQKCY AVRSNISEFL DIARRTYTEI VDDIAGMIAQ
LAEKYSLPLR TSFSSSRGFF IQMTTDCAAL SSDQLPSEFI KISKVKNSYS FTSADLIKMN
ERCQESLREI YHMTYMIVCK LLSEIYEHIH CLYKLSDTVS MLDMLLSFAH ACTLSDYVRP
EFTDTLAIKQ GWHPILEKIS AEKPVANNTY ITEGSNVLII TGPNMSGKST YLKQIALCQI
MAQIGSYVPA EYASFRIAAQ IFTRISTDDD IETNSSTFMK EMKEIAYILH NANDKSLILI
DELGRGTNTE EGIGISYAVC EHLLSIKAFT LFTTHFLELC HLDALYLNVE NMHFEVQHVK
NTSRNKDAIL YTYKLSRGLT EEKNYGLKAA EASSLPSSIV LDARDITTQI TRQILQNQRS
SPEMDRQRAV YHLATRLVQA ARNSQLEPDR LRTYLSNLKK KYAGDFPRAV GLPEKTEE
