MSH4_YEAST
ID MSH4_YEAST Reviewed; 878 AA.
AC P40965; D6VTM6;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 2.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=MutS protein homolog 4;
GN Name=MSH4; OrderedLocusNames=YFL003C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8001134; DOI=10.1016/0092-8674(94)90037-x;
RA Ross-Macdonald P., Roeder G.S.;
RT "Mutation of a meiosis-specific MutS homolog decreases crossing over but
RT not mismatch correction.";
RL Cell 79:1069-1080(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7670463; DOI=10.1038/ng0795-261;
RA Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S.,
RA Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H.,
RA Eki T.;
RT "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces
RT cerevisiae.";
RL Nat. Genet. 10:261-268(1995).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204511 / S288c / AB972;
RX PubMed=8789262;
RX DOI=10.1002/(sici)1097-0061(199601)12:1<77::aid-yea887>3.0.co;2-5;
RA Naitou M., Ozawa M., Sasanuma S., Kobayashi M., Hagiwara H., Shibata T.,
RA Hanaoka F., Watanabe K., Ono A., Yamazaki M., Tashiro H., Eki T.,
RA Murakami Y.;
RT "Sequencing of a 23 kb fragment from Saccharomyces cerevisiae chromosome
RT VI.";
RL Yeast 12:77-84(1996).
RN [5]
RP INTERACTION WITH MSH5.
RX PubMed=9374523; DOI=10.1074/jbc.272.48.30345;
RA Pochart P., Woltering D., Hollingsworth N.M.;
RT "Conserved properties between functionally distinct MutS homologs in
RT yeast.";
RL J. Biol. Chem. 272:30345-30349(1997).
CC -!- FUNCTION: Involved in meiotic recombination. Facilitate crossovers
CC between homologs during meiosis.
CC -!- SUBUNIT: Heterooligomer of MSH4 and MSH5. {ECO:0000269|PubMed:9374523}.
CC -!- INTERACTION:
CC P40965; Q12175: MSH5; NbExp=2; IntAct=EBI-11371, EBI-11377;
CC -!- MISCELLANEOUS: Two distinct classes of crossovers have been
CC demonstrated in budding yeast. Class I is MSH4/MSH5 dependent and
CC exhibits crossover interference. Class II is MUS81/MMS4 dependent and
CC exhibits no interference.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family.
CC {ECO:0000305}.
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DR EMBL; U13999; AAA65234.1; -; Genomic_DNA.
DR EMBL; D50617; BAA09235.1; -; Genomic_DNA.
DR EMBL; BK006940; DAA12436.1; -; Genomic_DNA.
DR PIR; A55201; A55201.
DR RefSeq; NP_116652.1; NM_001179963.1.
DR AlphaFoldDB; P40965; -.
DR SMR; P40965; -.
DR BioGRID; 31143; 285.
DR ComplexPortal; CPX-1704; MSH4-MSH5 complex.
DR DIP; DIP-2426N; -.
DR IntAct; P40965; 5.
DR MINT; P40965; -.
DR STRING; 4932.YFL003C; -.
DR iPTMnet; P40965; -.
DR PaxDb; P40965; -.
DR PRIDE; P40965; -.
DR EnsemblFungi; YFL003C_mRNA; YFL003C; YFL003C.
DR GeneID; 850545; -.
DR KEGG; sce:YFL003C; -.
DR SGD; S000001891; MSH4.
DR VEuPathDB; FungiDB:YFL003C; -.
DR eggNOG; KOG0220; Eukaryota.
DR GeneTree; ENSGT00550000074897; -.
DR HOGENOM; CLU_002472_7_3_1; -.
DR InParanoid; P40965; -.
DR OMA; AIAMLDM; -.
DR BioCyc; YEAST:G3O-30451-MON; -.
DR PRO; PR:P40965; -.
DR Proteomes; UP000002311; Chromosome VI.
DR RNAct; P40965; protein.
DR GO; GO:0062128; C:MutSgamma complex; IPI:SGD.
DR GO; GO:0000228; C:nuclear chromosome; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:SGD.
DR Gene3D; 3.30.420.110; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR011184; DNA_mismatch_repair_Msh2.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007861; DNA_mismatch_repair_MutS_clamp.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR007860; DNA_mmatch_repair_MutS_con_dom.
DR InterPro; IPR036678; MutS_con_dom_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR Pfam; PF05188; MutS_II; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF05190; MutS_IV; 1.
DR Pfam; PF00488; MutS_V; 1.
DR PIRSF; PIRSF005813; MSH2; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF53150; SSF53150; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; DNA-binding; Meiosis; Nucleotide-binding; Reference proteome.
FT CHAIN 1..878
FT /note="MutS protein homolog 4"
FT /id="PRO_0000115201"
FT REGION 22..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 634..641
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 811..812
FT /note="IH -> MD (in Ref. 1; AAA65234)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 878 AA; 99225 MW; BBA1CABF1A08954C CRC64;
MSESNLSSFI STNYFNLRSA ANSSNSISKP STKKSIRNQK SPTNISSWAL KKKTLQIAET
TWENNEKDST HSHYLMTGSM ASRTATSLSR YSTNASLLGP SIDCVLCCIY EVPRDISTRI
GLCIINCNTG QMYLSDFMDS QIYIRVVHKL QIYQPTEILI PSSSLAPTVS KLATMIKFNV
AETVKIEEGS RKCFNSQDGL AAITKYLMDD TKKDLKIEEI IDKTFALCAA SAAISYMEEI
ISKSSRNLNA FRKLRIQFEG TENTMLIDSK TVRGLELVEN KLDKNGISLW KFLDTTSTKM
GQRSLRNSIL QPLTDRGSIE MRLEALEELK ANDDLLQKLR LEMKSLPDLD KLFSRLLCIN
HSAIKPDQRI NYVLLLKETL QSVKSLKDAL NDQLIQSRLI SETKKIFNND AIMEIEKLIN
SCINEDCVWA SSAIQLLNQR SYAVKSDSNG LLDVSRQIYK EVKEEFFREV EDLTAKNKIN
LDHNYDSARG FYLRIKRQEF TDDVATLPDV FISRTIKKNY IECTTLNIIK KNARLKEVME
EILLLSEETV DELLDKIATH ISELFMIAEA VAILDLVCSF TYNLKENNYT IPIFTNNLLI
RDSRHPLLEK VLKNFVPNTI SSTKHSSSLQ IITGCNMSGK SVYLKQVALI CIMAQMGSGI
PALYGSFPVF KRLHARVCND SMELTSSNFG FEMKEMAYFL DDINTETLLI LDELGRGSSI
ADGFCVSLAV TEHLLRTEAT VFLSTHFQDI PKIMSKKPAV SHLHMDAVLL NDNSVKMNYQ
LTQKSVAIEN SGIRVVKKIF NPDIIAEAYN IHSLLKIAKA RTENEDSNGV VDQKTINQMK
RIHNLVAILK ECAGNEKEPL TLGKLKEINS DFIENFEE
