MSH5_ARATH
ID MSH5_ARATH Reviewed; 807 AA.
AC F4JEP5; A4URL6; Q56XI7; Q6NLQ0; Q9LTP4;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=DNA mismatch repair protein MSH5;
DE Short=AtMSH5;
DE AltName: Full=MutS protein homolog 5;
GN Name=MSH5; OrderedLocusNames=At3g20475; ORFNames=MQC12.27;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=18318687; DOI=10.1111/j.1365-313x.2008.03470.x;
RA Higgins J.D., Vignard J., Mercier R., Pugh A.G., Franklin F.C., Jones G.H.;
RT "AtMSH5 partners AtMSH4 in the class I meiotic crossover pathway in
RT Arabidopsis thaliana, but is not required for synapsis.";
RL Plant J. 55:28-39(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 448-807.
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=18379590; DOI=10.1038/cr.2008.44;
RA Lu X., Liu X., An L., Zhang W., Sun J., Pei H., Meng H., Fan Y., Zhang C.;
RT "The Arabidopsis MutS homolog AtMSH5 is required for normal meiosis.";
RL Cell Res. 18:589-599(2008).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18812090; DOI=10.1016/j.cub.2008.08.041;
RA Macaisne N., Novatchkova M., Peirera L., Vezon D., Jolivet S., Froger N.,
RA Chelysheva L., Grelon M., Mercier R.;
RT "SHOC1, an XPF endonuclease-related protein, is essential for the formation
RT of class I meiotic crossovers.";
RL Curr. Biol. 18:1432-1437(2008).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=21771883; DOI=10.1242/jcs.088229;
RA Macaisne N., Vignard J., Mercier R.;
RT "SHOC1 and PTD form an XPF-ERCC1-like complex that is required for
RT formation of class I crossovers.";
RL J. Cell Sci. 124:2687-2691(2011).
CC -!- FUNCTION: Involved in meiotic recombination in association with MSH4.
CC Required for reciprocal recombination and proper segregation of
CC homologous chromosomes at meiosis. Promotes homologous recombination
CC through facilitating chiasma formation during prophase I. Involved in
CC the control of class I crossovers formation.
CC {ECO:0000269|PubMed:18318687, ECO:0000269|PubMed:18379590,
CC ECO:0000269|PubMed:18812090, ECO:0000269|PubMed:21771883}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18318687}. Chromosome
CC {ECO:0000269|PubMed:18318687}. Note=In pollen mother cells during
CC meiosis, localizes to unsynapsed axes during leptotene and zygotene,
CC but is not present on synapsed regions of zygotene nuclei.
CC {ECO:0000269|PubMed:18318687}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in flowers.
CC {ECO:0000269|PubMed:18318687, ECO:0000269|PubMed:18379590}.
CC -!- DISRUPTION PHENOTYPE: Normal vegetative growth but severe reduction in
CC fertility due to a decrease in chiasma frequency at metaphase I of
CC meiosis. {ECO:0000269|PubMed:18318687, ECO:0000269|PubMed:18379590,
CC ECO:0000269|PubMed:18812090, ECO:0000269|PubMed:21771883}.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB02831.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAD95388.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAF02028.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; EF471448; ABO69626.1; -; mRNA.
DR EMBL; AB024036; BAB02831.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE76384.1; -; Genomic_DNA.
DR EMBL; AK221687; BAD95388.1; ALT_FRAME; mRNA.
DR EMBL; AK230222; BAF02028.1; ALT_FRAME; mRNA.
DR EMBL; BT012280; AAS76767.1; -; mRNA.
DR RefSeq; NP_188683.3; NM_112939.4.
DR AlphaFoldDB; F4JEP5; -.
DR SMR; F4JEP5; -.
DR STRING; 3702.AT3G20475.1; -.
DR PaxDb; F4JEP5; -.
DR ProteomicsDB; 250876; -.
DR EnsemblPlants; AT3G20475.1; AT3G20475.1; AT3G20475.
DR GeneID; 821593; -.
DR Gramene; AT3G20475.1; AT3G20475.1; AT3G20475.
DR KEGG; ath:AT3G20475; -.
DR Araport; AT3G20475; -.
DR TAIR; locus:2092404; AT3G20475.
DR eggNOG; KOG0217; Eukaryota.
DR eggNOG; KOG0221; Eukaryota.
DR HOGENOM; CLU_002472_8_2_1; -.
DR InParanoid; F4JEP5; -.
DR OMA; VIEYTAH; -.
DR OrthoDB; 138168at2759; -.
DR PRO; PR:F4JEP5; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4JEP5; baseline and differential.
DR Genevisible; F4JEP5; AT.
DR GO; GO:0000794; C:condensed nuclear chromosome; IDA:TAIR.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0043073; C:germ cell nucleus; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR GO; GO:0051026; P:chiasma assembly; IMP:TAIR.
DR GO; GO:0045143; P:homologous chromosome segregation; IMP:UniProtKB.
DR GO; GO:0010777; P:meiotic mismatch repair involved in reciprocal meiotic recombination; IDA:UniProtKB.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:TAIR.
DR GO; GO:0000712; P:resolution of meiotic recombination intermediates; IEA:EnsemblPlants.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR011184; DNA_mismatch_repair_Msh2.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF00488; MutS_V; 1.
DR PIRSF; PIRSF005813; MSH2; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chromosome; DNA-binding; Meiosis; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..807
FT /note="DNA mismatch repair protein MSH5"
FT /id="PRO_0000418368"
FT BINDING 569..576
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 75
FT /note="V -> I (in Ref. 1; ABO69626)"
FT /evidence="ECO:0000305"
FT CONFLICT 658
FT /note="G -> D (in Ref. 5; AAS76767)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 807 AA; 91123 MW; C9EB2394FBB1D7AC CRC64;
MEEMEDTETE PQVYMACIQH GRRVGVSYYD CSVRQLHVLE FWEEDCSDFT LINMVKYQAK
PSIIYASTKS EESFVAALQQ NDGTDETTMV KLVKSSTFSY EQAWHRLVYL RVTGMDDGLN
IKERICYLSS MMDVGSEVQV RVSGGLLAIL ESERIVETLE QNESGSASIA IDSVMEVPLN
KFLKLDAAAH EALQIFQTDK HPSHMGIGRA KEGFSVFGMM NKCATPMGRR LLRSWFMRPI
LDLEVLDRRL NAISFFISSV ELMASLRETL KSVKDISHLL KKFNSPTSLC TSNDWTAFLK
SISALLHVNK IFEVGVSESL REHMRRFNLD IIEKAGLCIS TELDYVYELV IGVIDVTRSK
ERGYQTLVKE GFCAELDELR QIYEELPEFL QEVSAMELEH FPHLHKEKLP PCIVYIQQIG
YLMCIFGEKL DETALNRLTE FEFAFSDMDG ETQRFFYHTS KTRELDNLLG DIYHKILDME
RAIIRDLLSH TLLFSAHLLK AVNFVAELDC ILSLACVAHQ NNYVRPVLTV ESLLDIRNGR
HVLQEMAVDT FIPNDTEIND NGRIHIITGP NYSGKSIYVK QVALIVFLSH IGSFVPADAA
TVGLTDRIFC AMGSKFMTAE QSTFMIDLHQ VGMMLRQATS RSLCLLDEFG KGTLTEDGIG
LLGGTISHFA TCAEPPRVVV CTHLTELLNE SCLPVSEKIK FYTMSVLRPD TESANMEEIV
FLYRLIPGQT LLSYGLHCAL LAGVPEEVVK RAAIVLDAFE SNNNVDKLSL DKISSQDQAF
KDAVDKFAEL DISKGDIHAF FQDIFTS
