MSH5_CAEEL
ID MSH5_CAEEL Reviewed; 1369 AA.
AC Q19272; A0A061AKP2; Q9NB29;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 27-MAY-2002, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=MutS protein homolog 5;
GN Name=msh-5 {ECO:0000312|WormBase:F09E8.3a};
GN ORFNames=F09E8.3 {ECO:0000312|WormBase:F09E8.3a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), AND FUNCTION.
RC STRAIN=Bristol N2;
RX PubMed=11014811; DOI=10.1093/genetics/156.2.617;
RA Kelly K.O., Dernburg A.F., Stanfield G.M., Villeneuve A.M.;
RT "Caenorhabditis elegans msh-5 is required for both normal and radiation-
RT induced meiotic crossing over but not for completion of meiosis.";
RL Genetics 156:617-630(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-933.
RX PubMed=9787078; DOI=10.1006/geno.1998.5447;
RA Winand N.J., Panzer J.A., Kolodner R.D.;
RT "Cloning and characterization of the human and Caenorhabditis elegans
RT homologs of the Saccharomyces cerevisiae MSH5 gene.";
RL Genomics 53:69-80(1998).
RN [4]
RP FUNCTION.
RX PubMed=23832114; DOI=10.1038/cdd.2013.68;
RA Silva N., Adamo A., Santonicola P., Martinez-Perez E., La Volpe A.;
RT "Pro-crossover factors regulate damage-dependent apoptosis in the
RT Caenorhabditis elegans germ line.";
RL Cell Death Differ. 20:1209-1218(2013).
RN [5]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=29521627; DOI=10.7554/elife.30789;
RA Zhang L., Koehler S., Rillo-Bohn R., Dernburg A.F.;
RT "A compartmentalized signaling network mediates crossover control in
RT meiosis.";
RL Elife 7:0-0(2018).
RN [6]
RP INTERACTION WITH BRC-1 AND BRD-1, AND SUBCELLULAR LOCATION.
RX PubMed=30383754; DOI=10.1371/journal.pgen.1007653;
RA Janisiw E., Dello Stritto M.R., Jantsch V., Silva N.;
RT "BRCA1-BARD1 associate with the synaptonemal complex and pro-crossover
RT factors and influence RAD-51 dynamics during Caenorhabditis elegans
RT meiosis.";
RL PLoS Genet. 14:e1007653-e1007653(2018).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=30379819; DOI=10.1371/journal.pgen.1007776;
RA Nguyen H., Labella S., Silva N., Jantsch V., Zetka M.;
RT "C. elegans ZHP-4 is required at multiple distinct steps in the formation
RT of crossovers and their transition to segregation competent chiasmata.";
RL PLoS Genet. 14:E1007776-E1007776(2018).
CC -!- FUNCTION: Crucial component in meiotic recombination, functioning at
CC some point after the initiation step of recombination. Plays a role in
CC promoting the crossover outcome of meiotic recombination events.
CC Required for formation of normal meiotic crossover, and crossover and
CC chiasmata generated by artificially made DNA breaks. Together with him-
CC 14 and zhp-3 plays a role in the activation of DNA damage-dependent
CC apoptosis at the DNA damage checkpoint in pachytene cells
CC (PubMed:23832114). {ECO:0000269|PubMed:11014811,
CC ECO:0000269|PubMed:23832114}.
CC -!- SUBUNIT: Heterooligomer of him-14 and msh-5 (By similarity). Interacts
CC with the brc-1-brd-1 heterodimer (PubMed:30383754).
CC {ECO:0000250|UniProtKB:Q12175, ECO:0000269|PubMed:30383754}.
CC -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000269|PubMed:29521627,
CC ECO:0000269|PubMed:30379819}. Note=From mid-pachytene, co-localizes
CC with cosa-1 and rmh-1 at crossover sites of early recombination
CC intermediates (PubMed:29521627, PubMed:30379819). Co-localizes with
CC brc-1 at crossover sites in mid-late pachytene nuclei
CC (PubMed:30383754). {ECO:0000269|PubMed:29521627,
CC ECO:0000269|PubMed:30379819, ECO:0000269|PubMed:30383754}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:F09E8.3a};
CC IsoId=Q19272-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:F09E8.3b};
CC IsoId=Q19272-2; Sequence=VSP_060676;
CC -!- TISSUE SPECIFICITY: Expressed in the germline.
CC {ECO:0000269|PubMed:29521627}.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family.
CC {ECO:0000305}.
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DR EMBL; AF271389; AAF76200.1; -; mRNA.
DR EMBL; AF070070; AAC70065.1; -; mRNA.
DR EMBL; BX284604; CAA98059.2; -; Genomic_DNA.
DR EMBL; BX284604; CDR32661.1; -; Genomic_DNA.
DR PIR; T20659; T20659.
DR PIR; T20660; T20660.
DR PIR; T43201; T43201.
DR RefSeq; NP_001293899.1; NM_001306970.1.
DR RefSeq; NP_502531.1; NM_070130.6. [Q19272-1]
DR AlphaFoldDB; Q19272; -.
DR SMR; Q19272; -.
DR BioGRID; 43359; 4.
DR STRING; 6239.F09E8.3; -.
DR PaxDb; Q19272; -.
DR EnsemblMetazoa; F09E8.3a.1; F09E8.3a.1; WBGene00003421. [Q19272-1]
DR EnsemblMetazoa; F09E8.3b.1; F09E8.3b.1; WBGene00003421. [Q19272-2]
DR GeneID; 178268; -.
DR KEGG; cel:CELE_F09E8.3; -.
DR UCSC; F09E8.3; c. elegans. [Q19272-1]
DR CTD; 178268; -.
DR WormBase; F09E8.3a; CE26678; WBGene00003421; msh-5. [Q19272-1]
DR WormBase; F09E8.3b; CE49987; WBGene00003421; msh-5. [Q19272-2]
DR eggNOG; KOG0221; Eukaryota.
DR GeneTree; ENSGT00550000074977; -.
DR HOGENOM; CLU_004671_0_0_1; -.
DR InParanoid; Q19272; -.
DR OMA; ACRIYKA; -.
DR OrthoDB; 138168at2759; -.
DR PhylomeDB; Q19272; -.
DR PRO; PR:Q19272; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00003421; Expressed in germ line (C elegans) and 3 other tissues.
DR ExpressionAtlas; Q19272; baseline and differential.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IMP:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR GO; GO:0051026; P:chiasma assembly; IMP:WormBase.
DR GO; GO:0045143; P:homologous chromosome segregation; IMP:WormBase.
DR GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:UniProtKB.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007861; DNA_mismatch_repair_MutS_clamp.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF05190; MutS_IV; 1.
DR Pfam; PF00488; MutS_V; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Chromosome; DNA-binding; Meiosis;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..1369
FT /note="MutS protein homolog 5"
FT /id="PRO_0000115205"
FT REGION 138..190
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 880..915
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 935..1135
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1153..1182
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1248..1278
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 148..165
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 880..897
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 898..915
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 972..1007
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1021..1042
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1050..1069
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1094..1135
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 639..646
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..446
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_060676"
SQ SEQUENCE 1369 AA; 153140 MW; DDC5FDAB7DB86C4A CRC64;
MSTRWRYYNS KRGNGFRGRG RGRGRGTSLT AVALPRDDNF HKGAQDGAYF KDMPMDPEQF
RDETVLSLSF AQGMLGAAYY EQSSQLLKIM NDISEDLEFR FLKRLIDDVK PTLIIANRSQ
DLEFIKFLTT RYDPQEKIYE DGTTEEGTSE DTVPTWDSSL AYSTDETTAE KEEKEEDEDD
DDEGLPAKLN KLPNNFFRMS RAIERLKAMA GSHDSSMTEE DKYIIIKMRF DIEAVNMIRS
FGALLLFLDE TRMGVTDDPL SVTSPIKSIK TFTLGNLVEI DFNTIQALDI LPKETENKKT
FGQGRSLYQL MDKCRSTVGK KCLRKWFRNP TTDRDDLVSR QKCVHYFKQD WNAEVTAKLS
SILGRVKALN SVFQKFQSGT AQLIHWECFV STVNALVEIL NIIRQTPISK EFPVESDLLR
EVSEIAVIAG SIINFAESKI QGRVTVMNGI DEELDEIRDT YENMPMVLTA IAKQEEARLG
LPPYSNVACV YIPLVGFVLS VPRDYGVESQ PDMTLLYSTH EDLRVRNATT SRLDDEFGDI
LMRLIDSQTA IILTLKTRVM KKKRSIIKLL SIASRIDVLI SFGLIAAQNG WNCPALVDEP
VIEAVELYHP ISVLVVKKSF VPNQVSSGRD GIKASIITGP NACGKSVYMK SIGIMVFLSH
IGSFVPARHA KIGIVDRIVT RMFTVDSVLD GMSTFAKDVE QVALALRKAT GNSLVIIDEF
GKGTMTEVGL SLLASVMTYW MNRGADRCPH IFLSSHFHAL PNYIPLETNI ATFLTFTVLR
EAGGKIKYLF RMTPGLVDCS FALSVAKEEG IPPPVIGRAC RIYKALKAGT LLKEIKAEVS
NDNEKQLVED MDVVLADEDG FMAAVESFVK RKKTSFCESS MRNVSEEIEK ERSEASTPAS
KSRSTITARS NSVLSSRSMA SVDQLSVLDA LLPKKKKKKV TGSSMESSMS PDPFQEEDEG
TEGEEDQISA PVSRPTLPSV QKYASEEEKQ QSINSRHSFS TRTAIHIPTP IQMGEAGGVK
RPRSTSTSSP GPSASKSVRT EVFKKTPNVK ESQVLETPKQ LSISSFLEPK FPSSEKDVIS
RVSERYLQSD PFKTPISDRR SQQSSRHSTP KNRSMNQSLI QSARDTPHET IRSSNEVNPE
FFNIFNFPDD SILKSQDTYD PNVTPRSSSR RELRPDVSHS QNSQFGEVFS ELGTQFSIFN
SQQSFPGNSM GTTNPDCSIF DDFFANSQDG EKKIDSTKTS MPIVNSDNFI FKTPEPRSSE
KQRSLLKNKG QASNSSISPS SLILGQLAFG DVDQTPRPRG DNPIEFQYDV VDDDDPIFEE
KNCSAPVFEF LKSNDDEEDD EFLKSFLETE GSLHIDTSAD ETIDRSKRS
