MSH5_MOUSE
ID MSH5_MOUSE Reviewed; 833 AA.
AC Q9QUM7; Q9Z1Q6;
DT 24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=MutS protein homolog 5;
GN Name=Msh5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ; TISSUE=Testis;
RX PubMed=10556423; DOI=10.1007/s003359901161;
RA Her C., Wu X., Wan W., Doggett N.A.;
RT "Identification and characterization of the mouse MutS homolog 5: Msh5.";
RL Mamm. Genome 10:1054-1061(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=129/Ola;
RX PubMed=9916805; DOI=10.1038/5075;
RA Edelmann W., Cohen P.E., Kneitz B., Winand N., Lia M., Heyer J.,
RA Kolodner R., Pollard J.W., Kucherlapati R.;
RT "Mammalian MutS homologue 5 is required for chromosome pairing in
RT meiosis.";
RL Nat. Genet. 21:123-127(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=B10.A;
RX PubMed=11797099; DOI=10.1007/s00251-001-0381-0;
RA van Kooij M., de Groot K., van Vugt H., Aten J., Snoek M.;
RT "Genotype versus phenotype: conflicting results in mapping a lung tumor
RT susceptibility locus to the G7c recombination interval in the mouse MHC
RT class III region.";
RL Immunogenetics 53:656-661(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=129;
RX PubMed=14656967; DOI=10.1101/gr.1736803;
RA Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D.,
RA Hood L.;
RT "Analysis of the gene-dense major histocompatibility complex class III
RT region and its comparison to mouse.";
RL Genome Res. 13:2621-2636(2003).
RN [5]
RP MUTAGENESIS OF ASP-486.
RX PubMed=28175301; DOI=10.1093/hmg/ddx044;
RA Guo T., Zhao S., Zhao S., Chen M., Li G., Jiao X., Wang Z., Zhao Y.,
RA Qin Y., Gao F., Chen Z.J.;
RT "Mutations in MSH5 in Primary Ovarian Insufficiency.";
RL Hum. Mol. Genet. 26:1452-1457(2017).
CC -!- FUNCTION: Involved in DNA mismatch repair and meiotic recombination
CC processes. Facilitates crossovers between homologs during meiosis (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterooligomer of MSH4 and MSH5. Interacts with HJURP (By
CC similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family.
CC {ECO:0000305}.
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DR EMBL; AF146227; AAF07881.1; -; mRNA.
DR EMBL; AF107352; AAD52102.1; -; mRNA.
DR EMBL; AF109905; AAC84154.1; -; Genomic_DNA.
DR EMBL; AF397035; AAL14454.1; -; Genomic_DNA.
DR EMBL; AF397036; AAL14462.1; -; Genomic_DNA.
DR CCDS; CCDS28674.1; -.
DR RefSeq; NP_001139687.1; NM_001146215.2.
DR RefSeq; NP_038628.2; NM_013600.3.
DR AlphaFoldDB; Q9QUM7; -.
DR SMR; Q9QUM7; -.
DR BioGRID; 201527; 1.
DR IntAct; Q9QUM7; 1.
DR MINT; Q9QUM7; -.
DR STRING; 10090.ENSMUSP00000007250; -.
DR iPTMnet; Q9QUM7; -.
DR PhosphoSitePlus; Q9QUM7; -.
DR PaxDb; Q9QUM7; -.
DR PRIDE; Q9QUM7; -.
DR ProteomicsDB; 295594; -.
DR DNASU; 17687; -.
DR GeneID; 17687; -.
DR KEGG; mmu:17687; -.
DR UCSC; uc008cfc.3; mouse.
DR CTD; 4439; -.
DR MGI; MGI:1329021; Msh5.
DR eggNOG; KOG0221; Eukaryota.
DR InParanoid; Q9QUM7; -.
DR OrthoDB; 138168at2759; -.
DR PhylomeDB; Q9QUM7; -.
DR TreeFam; TF314549; -.
DR BioGRID-ORCS; 17687; 1 hit in 110 CRISPR screens.
DR ChiTaRS; Msh5; mouse.
DR PRO; PR:Q9QUM7; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q9QUM7; protein.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000795; C:synaptonemal complex; IDA:MGI.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR GO; GO:0051026; P:chiasma assembly; IBA:GO_Central.
DR GO; GO:0007292; P:female gamete generation; IMP:MGI.
DR GO; GO:0007129; P:homologous chromosome pairing at meiosis; IMP:MGI.
DR GO; GO:0007127; P:meiosis I; IMP:MGI.
DR GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR011184; DNA_mismatch_repair_Msh2.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007861; DNA_mismatch_repair_MutS_clamp.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF05190; MutS_IV; 1.
DR Pfam; PF00488; MutS_V; 1.
DR PIRSF; PIRSF005813; MSH2; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Meiosis;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..833
FT /note="MutS protein homolog 5"
FT /id="PRO_0000115203"
FT REGION 1..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..33
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 591..598
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT MUTAGEN 486
FT /note="D->Y: Mice carrying this mutation show atrophic
FT ovaries without oocytes, but no other visible phenotype."
FT /evidence="ECO:0000269|PubMed:28175301"
FT CONFLICT 804
FT /note="K -> T (in Ref. 3 and 4)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 833 AA; 92602 MW; 73C6634698D991E2 CRC64;
MAFRATPGRT PPGPGPRSGI PSASFPSPQP PMAGPGGIEE EDEEEPAEIH LCVLWSSGYL
GIAYYDTSDS TIHFMPDAPD HESLKLLQRV LDEINPQSVV TSAKQDEAMT RFLGKLASEE
HREPKGPEII LLPSVDFGPE ISKQRLLSGN YSFISDSMTA TEKILFLSSI IPFDCVLTVR
ALGGLLKFLS RRRIGVELED YDVGVPILGF KKFVLTHLVS IDQDTYSVLQ IFKSESHPSV
YKVASGLKEG LSLFGILNRC RCKWGQKLLR LWFTRPTREL RELNSRLDVI QFFLMPQNLD
MAQMLHRLLS HIKNVPLILK RMKLSHTKVS DWQVLYKTVY SALGLRDACR SLPQSIQLFQ
DIAQEFSDDL HHIASLIGKV VDFEESLAEN RFTVLPNIDP DIDAKKRRLI GLPSFLTEVA
QKELENLDSR IPSCSVIYIP LIGFLLSIPR LPFMVEASDF EIEGLDFMFL SEDKLHYRSA
RTKELDTLLG DLHCEIRDQE TLLMYQLQCQ VLARASVLTR VLDLASRLDV LLALASAARD
YGYSRPHYSP CIHGVRIRNG RHPLMELCAR TFVPNSTDCG GDQGRVKVIT GPNSSGKSIY
LKQVGLITFM ALVGSFVPAE EAEIGVIDAI FTRIHSCESI SLGLSTFMID LNQVAKAVNN
ATEHSLVLID EFGKGTNSVD GLALLAAVLR HWLALGPSCP HVFVATNFLS LVQLQLLPQG
PLVQYLTMET CEDGEDLVFF YQLCQGVASA SHASHTAAQA GLPDPLIARG KEVSDLIRSG
KPIKATNELL RRNQMENCQA LVDKFLKLDL EDPTLDLDIF ISQEVLPAAP TIL
