MSH5_ORYSJ
ID MSH5_ORYSJ Reviewed; 809 AA.
AC Q6L4V0; A0A0P0WP46;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=DNA mismatch repair protein MSH5 {ECO:0000305};
DE Short=OsMSH5 {ECO:0000303|PubMed:23220939};
DE AltName: Full=MutS protein homolog 5 {ECO:0000305};
GN Name=MSH5 {ECO:0000303|PubMed:23220939};
GN OrderedLocusNames=Os05g0498300 {ECO:0000312|EMBL:BAF17862.1},
GN LOC_Os05g41880 {ECO:0000305};
GN ORFNames=P0010D04.9 {ECO:0000312|EMBL:AAT44285.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=23220939; DOI=10.1093/mp/sss145;
RA Luo Q., Tang D., Wang M., Luo W., Zhang L., Qin B., Shen Y., Wang K.,
RA Li Y., Cheng Z.;
RT "The role of OsMSH5 in crossover formation during rice meiosis.";
RL Mol. Plant 6:729-742(2013).
CC -!- FUNCTION: Involved in meiotic recombination. Required for reciprocal
CC recombination and proper segregation of homologous chromosomes at
CC meiosis. Promotes homologous recombination through facilitating chiasma
CC formation during prophase I. Involved in the control of class I
CC crossover (interference-sensitive crossover) formation.
CC {ECO:0000269|PubMed:23220939}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23220939}. Chromosome
CC {ECO:0000269|PubMed:23220939}. Note=Detected in punctuate foci onto the
CC chromosomes during the early meiotic prophase I.
CC {ECO:0000269|PubMed:23220939}.
CC -!- TISSUE SPECIFICITY: Highly expressed in the panicles.
CC {ECO:0000269|PubMed:23220939}.
CC -!- DISRUPTION PHENOTYPE: Normal vegetative growth but severe reduction in
CC fertility due to a decrease in chiasma frequency at metaphase I of
CC meiosis. {ECO:0000269|PubMed:23220939}.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family.
CC {ECO:0000305}.
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DR EMBL; AC130605; AAT44285.1; -; Genomic_DNA.
DR EMBL; AP008211; BAF17862.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS94731.1; -; Genomic_DNA.
DR RefSeq; XP_015639362.1; XM_015783876.1.
DR AlphaFoldDB; Q6L4V0; -.
DR SMR; Q6L4V0; -.
DR STRING; 4530.OS05T0498300-01; -.
DR PaxDb; Q6L4V0; -.
DR PRIDE; Q6L4V0; -.
DR EnsemblPlants; Os05t0498300-01; Os05t0498300-01; Os05g0498300.
DR GeneID; 4339215; -.
DR Gramene; Os05t0498300-01; Os05t0498300-01; Os05g0498300.
DR KEGG; osa:4339215; -.
DR eggNOG; KOG0221; Eukaryota.
DR HOGENOM; CLU_002472_8_2_1; -.
DR InParanoid; Q6L4V0; -.
DR OMA; VIEYTAH; -.
DR OrthoDB; 138168at2759; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR Genevisible; Q6L4V0; OS.
DR GO; GO:0000794; C:condensed nuclear chromosome; IEA:EnsemblPlants.
DR GO; GO:0043073; C:germ cell nucleus; IEA:EnsemblPlants.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR GO; GO:0051026; P:chiasma assembly; IMP:UniProtKB.
DR GO; GO:0045143; P:homologous chromosome segregation; IMP:UniProtKB.
DR GO; GO:0010777; P:meiotic mismatch repair involved in reciprocal meiotic recombination; IEA:EnsemblPlants.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:UniProtKB.
DR GO; GO:0000712; P:resolution of meiotic recombination intermediates; IMP:UniProtKB.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR017261; DNA_mismatch_repair_MutS/MSH.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF00488; MutS_V; 1.
DR PIRSF; PIRSF037677; DNA_mis_repair_Msh6; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chromosome; DNA-binding; Meiosis; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..809
FT /note="DNA mismatch repair protein MSH5"
FT /id="PRO_0000432122"
FT BINDING 572..579
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 809 AA; 91337 MW; A306B9ACD956727D CRC64;
MDEEEEEEMS EREVDSQVHM ACVMQGRRVG IAYYDSSMHQ LFVLEIWEDI TEDFPLIDLV
KYQSKPSTIY TSTKTDEALL LALQRNDCND EAPAVKLMKS STFSYEQAWH RLMYLKVAAM
DEGLSVKERI CFLNSMMDLG SDVQVRAAGG LLAILDNERL LDTLDQMEGG ASIAIDSVAQ
ISLDKFLKLD ATAHEALQIF QVDKHPSYMG IGRAKEGFSV FGMLNKCVTP MGKHLLRTWF
LRPIIDIDVI NNRLNTISFF LCCEDVMSAL RGTLKSVRDI PHMLKKFNSP SSFCTSSDWH
AFLKCICSLL HINKIFEVGI SEHLAIKLQH MNIDLVGKAN SSITEELDYV SDLVVGVIDV
QRGKEKGYDT LVKDGLCEEL DELRMVYEEL PDFLEQVSAN EIASFPFSFE CRKAPLIVYV
HQIGYLMCFF DEKISDALLI GLPDFEFAFS EEGEERRFYY HTQKTRELDN LLGDIYHKIL
DMERAIIRDL VCRVCQFIPQ LTKAVNFAAE LDCILSLAIV ARQNNYVRPI LTEDSILEIQ
NGRHALQEMT VDTFVPNDTK IRSSGRINII TGPNYSGKSI YIKQVALVVF LAHIGSFVPA
DSAIVGLTDR IFCAMGSKSM TSEQSTFMID LHQVGTMLRH ATSRSLCLLD EFGKGTLTED
GIGLLGGTIS HFTDYDCPPK VLLSTHLTQI FTESYLPQSE HIKCYTMSVL NPDEQTDNED
VIFLYRLVPG QALLSFGLHC AQLAGVPSEV VQRAVTVLGD IHSKRPIRRM VWEKLAAKDQ
QYQDAVTKLL AFDPHKGDLV NFFQEVFPS
