MSH5_YEAST
ID MSH5_YEAST Reviewed; 901 AA.
AC Q12175; D6VRJ6;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=MutS protein homolog 5;
GN Name=MSH5; OrderedLocusNames=YDL154W; ORFNames=D1542;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7622037; DOI=10.1101/gad.9.14.1728;
RA Hollingsworth N.M., Ponte L., Halsey C.;
RT "MSH5, a novel MutS homolog, facilitates meiotic reciprocal recombination
RT between homologs in Saccharomyces cerevisiae but not mismatch repair.";
RL Genes Dev. 9:1728-1739(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8972581;
RX DOI=10.1002/(sici)1097-0061(199612)12:15%3c1587::aid-yea46%3e3.0.co;2-6;
RA Delaveau T.T.D., Blugeon C., Jacq C., Perea J.;
RT "Analysis of a 23 kb region on the left arm of yeast chromosome IV.";
RL Yeast 12:1587-1592(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP INTERACTION WITH MSH4, AND MUTAGENESIS OF 643-GLY-ALA-644 AND GLY-648.
RX PubMed=9374523; DOI=10.1074/jbc.272.48.30345;
RA Pochart P., Woltering D., Hollingsworth N.M.;
RT "Conserved properties between functionally distinct MutS homologs in
RT yeast.";
RL J. Biol. Chem. 272:30345-30349(1997).
CC -!- FUNCTION: Involved in meiotic recombination. Facilitate crossovers
CC between homologs during meiosis.
CC -!- SUBUNIT: Heterooligomer of MSH4 and MSH5. {ECO:0000269|PubMed:9374523}.
CC -!- INTERACTION:
CC Q12175; P40965: MSH4; NbExp=2; IntAct=EBI-11377, EBI-11371;
CC -!- MISCELLANEOUS: Two distinct classes of crossovers have been
CC demonstrated in budding yeast. Class I is MSH4/MSH5 dependent and
CC exhibits crossover interference. Class II is MUS81/MMS4 dependent and
CC exhibits no interference.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family.
CC {ECO:0000305}.
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DR EMBL; L42517; AAA67649.1; -; Genomic_DNA.
DR EMBL; X97751; CAA66337.1; -; Genomic_DNA.
DR EMBL; Z74202; CAA98728.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11706.1; -; Genomic_DNA.
DR PIR; S67702; S67702.
DR RefSeq; NP_010127.1; NM_001180214.1.
DR AlphaFoldDB; Q12175; -.
DR SMR; Q12175; -.
DR BioGRID; 31909; 38.
DR ComplexPortal; CPX-1704; MSH4-MSH5 complex.
DR DIP; DIP-1457N; -.
DR IntAct; Q12175; 8.
DR MINT; Q12175; -.
DR STRING; 4932.YDL154W; -.
DR iPTMnet; Q12175; -.
DR PaxDb; Q12175; -.
DR PRIDE; Q12175; -.
DR EnsemblFungi; YDL154W_mRNA; YDL154W; YDL154W.
DR GeneID; 851401; -.
DR KEGG; sce:YDL154W; -.
DR SGD; S000002313; MSH5.
DR VEuPathDB; FungiDB:YDL154W; -.
DR eggNOG; KOG0221; Eukaryota.
DR GeneTree; ENSGT00550000074977; -.
DR HOGENOM; CLU_002472_8_0_1; -.
DR InParanoid; Q12175; -.
DR OMA; VIEYTAH; -.
DR BioCyc; YEAST:G3O-29549-MON; -.
DR PRO; PR:Q12175; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q12175; protein.
DR GO; GO:0062128; C:MutSgamma complex; IPI:SGD.
DR GO; GO:0000228; C:nuclear chromosome; IDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR GO; GO:0051026; P:chiasma assembly; IBA:GO_Central.
DR GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:SGD.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR011184; DNA_mismatch_repair_Msh2.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF00488; MutS_V; 1.
DR PIRSF; PIRSF005813; MSH2; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..901
FT /note="MutS protein homolog 5"
FT /id="PRO_0000115206"
FT BINDING 643..650
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT MUTAGEN 643..644
FT /note="GA->DV: Abolishes function; no effect on interaction
FT with MSH4."
FT /evidence="ECO:0000269|PubMed:9374523"
FT MUTAGEN 648
FT /note="G->R: Abolishes function; no effect on interaction
FT with MSH4."
FT /evidence="ECO:0000269|PubMed:9374523"
SQ SEQUENCE 901 AA; 102209 MW; 505E0344391E30E8 CRC64;
MSHEWLISAS ETMRSIGNGE GLRDKGAVVA NNDGEFNEGD TNREEDSSTI FSFDFDEEIV
MCIDFSGGKL GCSILDYHTK TLKAFDQDYV VNKTTISSHD LIDDADMSSN DISLLLGLLI
MEANPTVCLV PARLEDWIFD YIKTKCDEIN CRLELQPIKR FKKWDLLQSL QLRGHDNQTI
LNDILSNSKF TTTVTLGTVG CILANHEQLG EYNDSTASSN MVTGRLVQNA FEDVIHGIRY
IDIRDRMVLD ENTISALHIF PTAHKLGHDK MMRNGFFSVF ELFNQVSSDY ARRILKSWLI
NPLTNKKRIE TRYSIIRTLL DKQNAIIFSD LSQSIKRCPD AFGFINQLRS GKSTLGTWSK
VASFLEKGIA IFQLVSSLKL GSDEANILHD IKNKVDISAL KECLRKVETV IDFDTSRDTK
TLTINTGVDN RLDECRNIYN HLEGILLDVA RETQIFLLNT MPQEDCKTTK SLEKLVNAVY
IPQLGYLVTI SVLMEPLLDG IPNLQWEEIF RSSENIYFKN GRVLELDETY GDIYGAISDF
EIEILFSLQE QILRRKTQLT AYNILLSELE ILLSFAQVSA ERNYAEPQLV EDECILEIIN
GRHALYETFL DNYIPNSTMI DGGLFSELSW CEQNKGRIIV VTGANASGKS VYLTQNGLIV
YLAQIGCFVP AERARIGIAD KILTRIRTQE TVYKTQSSFL LDSQQMAKSL SLATEKSLIL
IDEYGKGTDI LDGPSLFGSI MLNMSKSEKC PRIIACTHFH ELFNENVLTE NIKGIKHYCT
DILISQKYNL LETAHVGEDH ESEGITFLFK VKEGISKQSF GIYCAKVCGL SRDIVERAEE
LSRMINRGDD VVQQCGNLTE KEMREFQKNQ EIVKKFLSWD LDLETTTTSE NLRLKLKNFL
R
