MSH6_CHICK
ID MSH6_CHICK Reviewed; 1337 AA.
AC E1BYJ2;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2013, sequence version 2.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=DNA mismatch repair protein Msh6 {ECO:0000250|UniProtKB:P54276};
GN Name=MSH6 {ECO:0000250|UniProtKB:P52701};
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Red jungle fowl;
RX PubMed=15592404; DOI=10.1038/nature03154;
RA Hillier L.W., Miller W., Birney E., Warren W., Hardison R.C., Ponting C.P.,
RA Bork P., Burt D.W., Groenen M.A.M., Delany M.E., Dodgson J.B.,
RA Chinwalla A.T., Cliften P.F., Clifton S.W., Delehaunty K.D., Fronick C.,
RA Fulton R.S., Graves T.A., Kremitzki C., Layman D., Magrini V.,
RA McPherson J.D., Miner T.L., Minx P., Nash W.E., Nhan M.N., Nelson J.O.,
RA Oddy L.G., Pohl C.S., Randall-Maher J., Smith S.M., Wallis J.W.,
RA Yang S.-P., Romanov M.N., Rondelli C.M., Paton B., Smith J., Morrice D.,
RA Daniels L., Tempest H.G., Robertson L., Masabanda J.S., Griffin D.K.,
RA Vignal A., Fillon V., Jacobbson L., Kerje S., Andersson L.,
RA Crooijmans R.P., Aerts J., van der Poel J.J., Ellegren H., Caldwell R.B.,
RA Hubbard S.J., Grafham D.V., Kierzek A.M., McLaren S.R., Overton I.M.,
RA Arakawa H., Beattie K.J., Bezzubov Y., Boardman P.E., Bonfield J.K.,
RA Croning M.D.R., Davies R.M., Francis M.D., Humphray S.J., Scott C.E.,
RA Taylor R.G., Tickle C., Brown W.R.A., Rogers J., Buerstedde J.-M.,
RA Wilson S.A., Stubbs L., Ovcharenko I., Gordon L., Lucas S., Miller M.M.,
RA Inoko H., Shiina T., Kaufman J., Salomonsen J., Skjoedt K., Wong G.K.-S.,
RA Wang J., Liu B., Wang J., Yu J., Yang H., Nefedov M., Koriabine M.,
RA Dejong P.J., Goodstadt L., Webber C., Dickens N.J., Letunic I., Suyama M.,
RA Torrents D., von Mering C., Zdobnov E.M., Makova K., Nekrutenko A.,
RA Elnitski L., Eswara P., King D.C., Yang S.-P., Tyekucheva S.,
RA Radakrishnan A., Harris R.S., Chiaromonte F., Taylor J., He J.,
RA Rijnkels M., Griffiths-Jones S., Ureta-Vidal A., Hoffman M.M., Severin J.,
RA Searle S.M.J., Law A.S., Speed D., Waddington D., Cheng Z., Tuzun E.,
RA Eichler E., Bao Z., Flicek P., Shteynberg D.D., Brent M.R., Bye J.M.,
RA Huckle E.J., Chatterji S., Dewey C., Pachter L., Kouranov A.,
RA Mourelatos Z., Hatzigeorgiou A.G., Paterson A.H., Ivarie R., Brandstrom M.,
RA Axelsson E., Backstrom N., Berlin S., Webster M.T., Pourquie O.,
RA Reymond A., Ucla C., Antonarakis S.E., Long M., Emerson J.J., Betran E.,
RA Dupanloup I., Kaessmann H., Hinrichs A.S., Bejerano G., Furey T.S.,
RA Harte R.A., Raney B., Siepel A., Kent W.J., Haussler D., Eyras E.,
RA Castelo R., Abril J.F., Castellano S., Camara F., Parra G., Guigo R.,
RA Bourque G., Tesler G., Pevzner P.A., Smit A., Fulton L.A., Mardis E.R.,
RA Wilson R.K.;
RT "Sequence and comparative analysis of the chicken genome provide unique
RT perspectives on vertebrate evolution.";
RL Nature 432:695-716(2004).
RN [2]
RP FUNCTION.
RX PubMed=23314153; DOI=10.1093/nar/gks1470;
RA Campo V.A., Patenaude A.M., Kaden S., Horb L., Firka D., Jiricny J.,
RA Di Noia J.M.;
RT "MSH6- or PMS2-deficiency causes re-replication in DT40 B cells, but it has
RT little effect on immunoglobulin gene conversion or on repair of AID-
RT generated uracils.";
RL Nucleic Acids Res. 41:3032-3046(2013).
CC -!- FUNCTION: Component of the post-replicative DNA mismatch repair system
CC (MMR). Involved in B cell growth by positively regulating B cell
CC proliferation and controlling replication efficiency. Controls cell
CC cycle to prevent re-replication and defects in DNA damage-induced G2
CC checkpoint. Doesn't seem to counteract or control the immunoglobulin
CC gene conversion (Ig GC) and to contribute to guanine/uracil mismatch
CC repair. {ECO:0000269|PubMed:23314153}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P52701}.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family.
CC {ECO:0000305}.
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DR EMBL; AADN03003073; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; E1BYJ2; -.
DR SMR; E1BYJ2; -.
DR STRING; 9031.ENSGALP00000038835; -.
DR PaxDb; E1BYJ2; -.
DR VEuPathDB; HostDB:geneid_421291; -.
DR eggNOG; KOG0217; Eukaryota.
DR HOGENOM; CLU_002472_1_3_1; -.
DR Proteomes; UP000000539; Unplaced.
DR GO; GO:0032301; C:MutSalpha complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0030983; F:mismatched DNA binding; IBA:GO_Central.
DR GO; GO:0006298; P:mismatch repair; IBA:GO_Central.
DR GO; GO:0007095; P:mitotic G2 DNA damage checkpoint signaling; IMP:UniProtKB.
DR GO; GO:0032876; P:negative regulation of DNA endoreduplication; IMP:UniProtKB.
DR GO; GO:0030890; P:positive regulation of B cell proliferation; IMP:UniProtKB.
DR Gene3D; 3.30.420.110; -; 1.
DR Gene3D; 3.40.1170.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR007695; DNA_mismatch_repair_MutS-lik_N.
DR InterPro; IPR017261; DNA_mismatch_repair_MutS/MSH.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007861; DNA_mismatch_repair_MutS_clamp.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR016151; DNA_mismatch_repair_MutS_N.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR007860; DNA_mmatch_repair_MutS_con_dom.
DR InterPro; IPR036678; MutS_con_dom_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000313; PWWP_dom.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR Pfam; PF01624; MutS_I; 1.
DR Pfam; PF05188; MutS_II; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF05190; MutS_IV; 1.
DR Pfam; PF00488; MutS_V; 1.
DR Pfam; PF00855; PWWP; 1.
DR PIRSF; PIRSF037677; DNA_mis_repair_Msh6; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SMART; SM00293; PWWP; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF53150; SSF53150; 1.
DR SUPFAM; SSF55271; SSF55271; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
DR PROSITE; PS50812; PWWP; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA-binding; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..1337
FT /note="DNA mismatch repair protein Msh6"
FT /id="PRO_0000436927"
FT DOMAIN 68..130
FT /note="PWWP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00162"
FT REGION 170..310
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 175..189
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 221..252
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 256..270
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 1111..1118
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1337 AA; 149891 MW; 812A3FC936697810 CRC64;
MMSASNLSVI HEVLLVLLNH QRINLPYENL EASLSRMLFL SSFVQEVVQV PIPCNVSANR
SVSGVYSPGD LVWAKMEGYP WWPCLIYNHP TEGTIVRGKG SSARIHVQFF DVSPTRGWVS
IKYLRPYKGS SDREVLKGGM FYSMKPEIKK AMELADDAMS KDKTKRLELA VCSEPSDTEE
AEEEEMEQMS GSASGDSDDS NSEEDVKGNK RVPNRGSAIK AKRRRVLDSD SDRDGSDVEF
KPDVKEASSE EASSGVDENE ATDVETDEES IEESPIKVPS KRKRGNVSKP SKRSSLENEH
SEAPKRAAPV SLEAKSKLTL FAAPENFESQ ANACSGGTNG FAAWEHEKLE WLQEGKKKDA
HRRRQNHPDY DPCTLYVPED YLNKCTPGMR RWWQLKSQNF DAVICYKVGK FYELYHMDAV
TGVNELGLIF MKGSWAHSGF PETAFGRFSA ILVQKGYKIA RVEQTETPEM MEARCKATAH
TTKFDKVVRR EICRIITKGT QTYSIIDCDP TENHNKYLLC VKEKEDSSGQ RVYGVCFVDT
SVGKFYVGQF SDDRHCSRFR TLVAHYTPVQ VLFEKGNLTV DTQKILKGSL ISCIQEGLIS
GSQFWSASKT LKVLLEEEYF KENQNTESGC VLPSVIKSLT SESDSLGLTP GENSELALSA
LGGIVFYLKK CLIDQELLSL ANFEKYIPVD ADNAKTVSSS NFFARTDRRM VLDGVTLMNL
EVLQNGTNGT TEGTLLERID SCCTPFGKRL LKQWLCAPLC NPTSINDRLD AVEDLLAVPA
KLTEITEHLK KLPDLERLLS KIHSIGSPLK SQNHPDSRAI FYEEIKYSKK KIADFLSALE
GFKVMNEIVD AMEEVASDFK SQVLKQLVTR KAKHPDGRFP DLSAELKRWD TAFDHNQARK
TGVITPKAGF DPDYDKALQD IKTVEEDFRT YLDKQRKLLG LKSVLYWGTG KNRYQMEIPE
TATSRNLPEE YELKSTRKGY KRYWTKEIEK MLAELINAEE RRDAALKDCM RRLFYNFDKN
SQDWQTAVQC IAVLDVLMSL ANYSQDGDGP LCRPVILLPV DSAPPFLELK NARHPCITKT
FFGDDFIPND IVIGSKDEDG GSEASCVLVT GPNMGGKSTL MRQAGLLVIM AQLGCYVPAE
VCRLTPIDRV FTRLGASDRI MSGESTFFVE LSETSSILQH ATEHSLVLVD ELGRGTATFD
GTAIASAVVR ELAENIKCRT LFSTHYHSLV EDYSGSAAVR LGHMACMVEN ESEDPSQETI
TFLYKFIEGA CPKSYGFNAA RLADIPEEII QKGHRKAKEF EKKTMSLRIF RFLCRVVDGV
THDANAVGKL TTMLSHL
