MSH6_DROME
ID MSH6_DROME Reviewed; 1190 AA.
AC Q9VUM0; B6IDV3;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 21-FEB-2001, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Probable DNA mismatch repair protein Msh6;
GN Name=Msh6; ORFNames=CG7003;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION.
RX PubMed=17339220; DOI=10.1534/genetics.107.070367;
RA Radford S.J., Sabourin M.M., McMahan S., Sekelsky J.;
RT "Meiotic recombination in Drosophila Msh6 mutants yields discontinuous gene
RT conversion tracts.";
RL Genetics 176:53-62(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-7; SER-8; THR-12; THR-14;
RP THR-16; SER-22; SER-24; THR-33; SER-95 AND SER-97, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Involved in post-replicative DNA-mismatch repair.
CC {ECO:0000269|PubMed:17339220}.
CC -!- SUBUNIT: Heterodimer of Msh2/Spel and Msh6. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family.
CC {ECO:0000305}.
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DR EMBL; AE014296; AAF49656.2; -; Genomic_DNA.
DR EMBL; BT050543; ACJ13250.1; -; mRNA.
DR RefSeq; NP_648755.1; NM_140498.2.
DR AlphaFoldDB; Q9VUM0; -.
DR SMR; Q9VUM0; -.
DR BioGRID; 64976; 3.
DR DIP; DIP-23712N; -.
DR IntAct; Q9VUM0; 4.
DR STRING; 7227.FBpp0075399; -.
DR iPTMnet; Q9VUM0; -.
DR PaxDb; Q9VUM0; -.
DR PRIDE; Q9VUM0; -.
DR EnsemblMetazoa; FBtr0075646; FBpp0075399; FBgn0036486.
DR GeneID; 39654; -.
DR KEGG; dme:Dmel_CG7003; -.
DR UCSC; CG7003-RA; d. melanogaster.
DR CTD; 2956; -.
DR FlyBase; FBgn0036486; Msh6.
DR VEuPathDB; VectorBase:FBgn0036486; -.
DR eggNOG; KOG0217; Eukaryota.
DR GeneTree; ENSGT00550000075024; -.
DR HOGENOM; CLU_002472_1_3_1; -.
DR InParanoid; Q9VUM0; -.
DR OMA; TPMMAQY; -.
DR OrthoDB; 138168at2759; -.
DR PhylomeDB; Q9VUM0; -.
DR Reactome; R-DME-5358565; Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
DR SignaLink; Q9VUM0; -.
DR BioGRID-ORCS; 39654; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 39654; -.
DR PRO; PR:Q9VUM0; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0036486; Expressed in secondary oocyte and 21 other tissues.
DR Genevisible; Q9VUM0; DM.
DR GO; GO:0032301; C:MutSalpha complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0030983; F:mismatched DNA binding; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; ISS:FlyBase.
DR GO; GO:0000710; P:meiotic mismatch repair; IMP:FlyBase.
DR GO; GO:0006298; P:mismatch repair; IBA:GO_Central.
DR Gene3D; 3.30.420.110; -; 1.
DR Gene3D; 3.40.1170.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR007695; DNA_mismatch_repair_MutS-lik_N.
DR InterPro; IPR017261; DNA_mismatch_repair_MutS/MSH.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007861; DNA_mismatch_repair_MutS_clamp.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR016151; DNA_mismatch_repair_MutS_N.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR007860; DNA_mmatch_repair_MutS_con_dom.
DR InterPro; IPR036678; MutS_con_dom_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR Pfam; PF01624; MutS_I; 1.
DR Pfam; PF05188; MutS_II; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF05190; MutS_IV; 1.
DR Pfam; PF00488; MutS_V; 1.
DR PIRSF; PIRSF037677; DNA_mis_repair_Msh6; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF53150; SSF53150; 1.
DR SUPFAM; SSF55271; SSF55271; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Nucleotide-binding;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..1190
FT /note="Probable DNA mismatch repair protein Msh6"
FT /id="PRO_0000115209"
FT REGION 1..174
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 230..249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 824..843
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..22
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 30..112
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 113..128
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 129..146
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 159..174
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 958..965
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT MOD_RES 7
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 8
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 12
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 14
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 16
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 22
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 24
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 33
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 95
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 97
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 1190 AA; 133136 MW; 1A416CE869EA5996 CRC64;
MSKKLNTSVG GTPTNTLLNY FSKSPAFDKK KLTPSVKTDP DASKSEKENL QNQQPKVKDG
KKEASKPAAK RKLPISDDEP ASGQRKRKRI VQPESDSEPE MEVTKSEDDF SDCASDYEPD
ENEASDDSVS SGAEEVSPSE NDMSVDSPTP KKSRKKSKIL NNNNNNEPSS KKVKLESTIQ
LAEGATFQEK LKNLQSNAKQ DASYDDIVTN TSNLDEPVVW PHQKLEFLQP DKIKDKEGRR
PDHPDYDKST LHVPEKFLNG LSPGVRQWWV LKSDNYDCVL FFKVGKFYEL YHMDADVGVN
ELGFTYMRGE FAHSGFPEIS FDKMSTILVD RGFKVARVEQ TETPDMMTER CKRIKATKFD
KVVAREICQI TNRGTQVFGS QCKIGPNHQP NYMLAIVEKD EGTCSRYGVC FIDTSIGDFH
LGEFEDDKNC SRLLTLVSHH MPVLFLNEKS ALSQRTQQIV RTVLGGILKE PVPGNGKHAC
SAEKTLKLLA ERYYAGPGSD DNWPLVLRTM QSDMDHLGLT PNDNYKLALK ALGQCIFFIH
KCKLEPKVLP MARYQLYVPP DQLADAKPAV ASTLRRSHMV LDATTLSNLR IIGEEHSLLS
TLDHCCTKFG KRLLHHWLCA PSCDVSVIKE RQDAIGELIR MPTELQEVRA LLAPMPDFER
NLAQIHLFGN KQIKQMDHPD SRAILFEEKL YNKQKLQGFM AVLKGFNDLT KLPTMFHQCK
TTLLKRITQL PESGGSFPDL SKELQYFATA FDHDAAAKTG VIAPQAGMDA EYDAAMDSIG
EVEKRLKTYL VEQERHFGCR ITYFGSDKKR YQLDVPESHA SKANKSYTLE GQTKGKKPSR
RYTTAETRAL LKDMQHAEDT RNMVLKDLAR RLFEKFSNHY DQWKQCIDCV ANLDVLGSLA
EYAGQQMVIC VPELVSDADQ PFIQLEEGYH PCANASTYIP NGLELGTASE APLSLLTGPN
MGGKSTLMRE VGLLVIMAQI GAHIPAASCR LSLVDRIFTR LGAQDDILAG HSTFLVELNE
TSLILKHATC HSLVLLDELG RGTATYDGTA IAASVVNFLA NLKCRTLFST HYHNLIDFFH
NDKRITLGHM ACMVENEDNA DPTQETVTFL YKYTAGACPK SYGFNAAKLA GMPQGIIKRA
YELSKKVEAI ALQRKITAKI VAATAGNEDT KKEKINALKD LLKQLKMCQV
