MSH7_ARATH
ID MSH7_ARATH Reviewed; 1109 AA.
AC Q9SMV7; O22372; Q9SNZ4;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=DNA mismatch repair protein MSH7;
DE Short=AtMSH7;
DE AltName: Full=MutS protein homolog 7;
GN Name=MSH7; Synonyms=MSH6-2; OrderedLocusNames=At3g24495; ORFNames=MOB24.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10517319; DOI=10.1007/pl00008640;
RA Ade J., Belzile F., Philippe H., Doutriaux M.P.;
RT "Four mismatch repair paralogues coexist in Arabidopsis thaliana: AtMSH2,
RT AtMSH3, AtMSH6-1 and AtMSH6-2.";
RL Mol. Gen. Genet. 262:239-249(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INTERACTION WITH MSH2.
RC STRAIN=cv. Columbia;
RX PubMed=10852942; DOI=10.2307/3871224;
RA Culligan K.M., Hays J.B.;
RT "Arabidopsis MutS homologs-AtMSH2, AtMSH3, AtMSH6, and a novel AtMSH7-form
RT three distinct protein heterodimers with different specificities for
RT mismatched DNA.";
RL Plant Cell 12:991-1002(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 852-937.
RC STRAIN=cv. Columbia;
RX PubMed=9342879; DOI=10.1104/pp.115.2.833;
RA Culligan K.M., Hays J.B.;
RT "DNA mismatch repair in plants. An Arabidopsis thaliana gene that predicts
RT a protein belonging to the MSH2 subfamily of eukaryotic MutS homologs.";
RL Plant Physiol. 115:833-839(1997).
RN [6]
RP FUNCTION, AND INTERACTION WITH MSH2.
RX PubMed=14530450; DOI=10.1093/nar/gkg780;
RA Wu S.Y., Culligan K., Lamers M., Hays J.;
RT "Dissimilar mispair-recognition spectra of Arabidopsis DNA-mismatch-repair
RT proteins MSH2*MSH6 (MutSalpha) and MSH2*MSH7 (MutSgamma).";
RL Nucleic Acids Res. 31:6027-6034(2003).
CC -!- FUNCTION: Component of the post-replicative DNA mismatch repair system
CC (MMR). Forms the heterodimer MutS gamma (MSH2-MSH7 heterodimer) which
CC binds to DNA mismatches thereby initiating DNA repair. MutS gamma
CC recognizes specifically the T/G single base mismatch, but not
CC trinucleotide insertion-deletion loops (IDL).
CC {ECO:0000269|PubMed:10852942, ECO:0000269|PubMed:14530450}.
CC -!- SUBUNIT: Heterodimer consisting of MSH2-MSH7 (MutS gamma).
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family.
CC {ECO:0000305}.
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DR EMBL; AJ007792; CAA07685.1; -; Genomic_DNA.
DR EMBL; AF193018; AAF06013.1; -; mRNA.
DR EMBL; AB020746; BAB01996.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76904.1; -; Genomic_DNA.
DR EMBL; AF009657; AAB82654.1; -; mRNA.
DR RefSeq; NP_850630.1; NM_180299.1.
DR AlphaFoldDB; Q9SMV7; -.
DR SMR; Q9SMV7; -.
DR BioGRID; 7371; 1.
DR STRING; 3702.AT3G24495.1; -.
DR PaxDb; Q9SMV7; -.
DR PRIDE; Q9SMV7; -.
DR ProteomicsDB; 250779; -.
DR EnsemblPlants; AT3G24495.1; AT3G24495.1; AT3G24495.
DR GeneID; 822040; -.
DR Gramene; AT3G24495.1; AT3G24495.1; AT3G24495.
DR KEGG; ath:AT3G24495; -.
DR Araport; AT3G24495; -.
DR TAIR; locus:1005716529; AT3G24495.
DR eggNOG; KOG0217; Eukaryota.
DR HOGENOM; CLU_002472_5_0_1; -.
DR InParanoid; Q9SMV7; -.
DR OMA; MASKFEW; -.
DR OrthoDB; 138168at2759; -.
DR PhylomeDB; Q9SMV7; -.
DR PRO; PR:Q9SMV7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SMV7; baseline and differential.
DR Genevisible; Q9SMV7; AT.
DR GO; GO:0032301; C:MutSalpha complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0030983; F:mismatched DNA binding; IBA:GO_Central.
DR GO; GO:0006298; P:mismatch repair; IBA:GO_Central.
DR Gene3D; 3.30.420.110; -; 1.
DR Gene3D; 3.40.1170.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR007695; DNA_mismatch_repair_MutS-lik_N.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR016151; DNA_mismatch_repair_MutS_N.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR007860; DNA_mmatch_repair_MutS_con_dom.
DR InterPro; IPR036678; MutS_con_dom_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR Pfam; PF01624; MutS_I; 1.
DR Pfam; PF05188; MutS_II; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF00488; MutS_V; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF53150; SSF53150; 1.
DR SUPFAM; SSF55271; SSF55271; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Nucleotide-binding;
KW Nucleus; Reference proteome.
FT CHAIN 1..1109
FT /note="DNA mismatch repair protein MSH7"
FT /id="PRO_0000115211"
FT REGION 19..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 61..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 853..860
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 573..579
FT /note="GPSGTLY -> VLQGPLN (in Ref. 2; AAF06013)"
FT /evidence="ECO:0000305"
FT CONFLICT 583
FT /note="D -> E (in Ref. 2; AAF06013)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1109 AA; 122270 MW; 9FECC916A324511C CRC64;
MQRQRSILSF FQKPTAATTK GLVSGDAASG GGGSGGPRFN VKEGDAKGDA SVRFAVSKSV
DEVRGTDTPP EKVPRRVLPS GFKPAESAGD ASSLFSNIMH KFVKVDDRDC SGERSREDVV
PLNDSSLCMK ANDVIPQFRS NNGKTQERNH AFSFSGRAEL RSVEDIGVDG DVPGPETPGM
RPRASRLKRV LEDEMTFKED KVPVLDSNKR LKMLQDPVCG EKKEVNEGTK FEWLESSRIR
DANRRRPDDP LYDRKTLHIP PDVFKKMSAS QKQYWSVKSE YMDIVLFFKV GKFYELYELD
AELGHKELDW KMTMSGVGKC RQVGISESGI DEAVQKLLAR GYKVGRIEQL ETSDQAKARG
ANTIIPRKLV QVLTPSTASE GNIGPDAVHL LAIKEIKMEL QKCSTVYGFA FVDCAALRFW
VGSISDDASC AALGALLMQV SPKEVLYDSK GLSREAQKAL RKYTLTGSTA VQLAPVPQVM
GDTDAAGVRN IIESNGYFKG SSESWNCAVD GLNECDVALS ALGELINHLS RLKLEDVLKH
GDIFPYQVYR GCLRIDGQTM VNLEIFNNSC DGGPSGTLYK YLDNCVSPTG KRLLRNWICH
PLKDVESINK RLDVVEEFTA NSESMQITGQ YLHKLPDLER LLGRIKSSVR SSASVLPALL
GKKVLKQRVK AFGQIVKGFR SGIDLLLALQ KESNMMSLLY KLCKLPILVG KSGLELFLSQ
FEAAIDSDFP NYQNQDVTDE NAETLTILIE LFIERATQWS EVIHTISCLD VLRSFAIAAS
LSAGSMARPV IFPESEATDQ NQKTKGPILK IQGLWHPFAV AADGQLPVPN DILLGEARRS
SGSIHPRSLL LTGPNMGGKS TLLRATCLAV IFAQLGCYVP CESCEISLVD TIFTRLGASD
RIMTGESTFL VECTETASVL QNATQDSLVI LDELGRGTST FDGYAIAYSV FRHLVEKVQC
RMLFATHYHP LTKEFASHPR VTSKHMACAF KSRSDYQPRG CDQDLVFLYR LTEGACPESY
GLQVALMAGI PNQVVETASG AAQAMKRSIG ENFKSSELRS EFSSLHEDWL KSLVGISRVA
HNNAPIGEDD YDTLFCLWHE IKSSYCVPK
