MSI1_ARATH
ID MSI1_ARATH Reviewed; 424 AA.
AC O22467;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Histone-binding protein MSI1;
DE AltName: Full=CAF-1 p48 homolog;
DE AltName: Full=Chromatin assembly factor 1 subunit MSI1;
DE Short=CAF-1 subunit MSI1;
DE AltName: Full=Protein MULTICOPY SUPPRESSOR OF IRA 1;
DE Short=AtMSI1;
DE AltName: Full=Protein medicis;
DE AltName: Full=WD-40 repeat-containing protein MSI1;
GN Name=MSI1; OrderedLocusNames=At5g58230; ORFNames=MCK7.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9338962; DOI=10.2307/3870446;
RA Ach R.A., Taranto P., Gruissem W.;
RT "A conserved family of WD-40 proteins binds to the retinoblastoma protein
RT in both plants and animals.";
RL Plant Cell 9:1595-1606(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, AND INTERACTION WITH FAS1 AND FAS2.
RX PubMed=11163246; DOI=10.1016/s0092-8674(01)00197-0;
RA Kaya H., Shibahara K., Taoka K., Iwabuchi M., Stillman B., Araki T.;
RT "FASCIATA genes for chromatin assembly factor-1 in Arabidopsis maintain the
RT cellular organization of apical meristems.";
RL Cell 104:131-142(2001).
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12736201; DOI=10.1242/dev.00470;
RA Hennig L., Taranto P., Walser M., Schoenrock N., Gruissem W.;
RT "Arabidopsis MSI1 is required for epigenetic maintenance of reproductive
RT development.";
RL Development 130:2555-2565(2003).
RN [7]
RP FUNCTION, AND INTERACTION WITH MEA AND FIE.
RX PubMed=12970192; DOI=10.1093/emboj/cdg444;
RA Koehler C., Hennig L., Bouveret R., Gheyselinck J., Grossniklaus U.,
RA Gruissem W.;
RT "Arabidopsis MSI1 is a component of the MEA/FIE Polycomb group complex and
RT required for seed development.";
RL EMBO J. 22:4804-4814(2003).
RN [8]
RP FUNCTION.
RX PubMed=15151989; DOI=10.1242/dev.01168;
RA Guitton A.-E., Page D.R., Chambrier P., Lionnet C., Faure J.-E.,
RA Grossniklaus U., Berger F.;
RT "Identification of new members of fertilisation independent seed Polycomb
RT group pathway involved in the control of seed development in Arabidopsis
RT thaliana.";
RL Development 131:2971-2981(2004).
RN [9]
RP FUNCTION.
RX PubMed=15854908; DOI=10.1016/j.cub.2005.02.066;
RA Guitton A.-E., Berger F.;
RT "Loss of function of MULTICOPY SUPPRESSOR OF IRA 1 produces nonviable
RT parthenogenetic embryos in Arabidopsis.";
RL Curr. Biol. 15:750-754(2005).
RN [10]
RP FUNCTION.
RX PubMed=17021044; DOI=10.1242/dev.02599;
RA Exner V., Taranto P., Schoenrock N., Gruissem W., Hennig L.;
RT "Chromatin assembly factor CAF-1 is required for cellular differentiation
RT during plant development.";
RL Development 133:4163-4172(2006).
RN [11]
RP FUNCTION.
RX PubMed=16452472; DOI=10.1074/jbc.m513426200;
RA Schoenrock N., Exner V., Probst A., Gruissem W., Hennig L.;
RT "Functional genomic analysis of CAF-1 mutants in Arabidopsis thaliana.";
RL J. Biol. Chem. 281:9560-9568(2006).
RN [12]
RP FUNCTION, INTERACTION WITH RBR1, AND DEVELOPMENTAL STAGE.
RX PubMed=18700816; DOI=10.1371/journal.pbio.0060194;
RA Jullien P.E., Mosquna A., Ingouff M., Sakata T., Ohad N., Berger F.;
RT "Retinoblastoma and its binding partner MSI1 control imprinting in
RT Arabidopsis.";
RL PLoS Biol. 6:E194-E194(2008).
RN [13]
RP DWD MOTIF.
RX PubMed=18223036; DOI=10.1105/tpc.107.055418;
RA Lee J.H., Terzaghi W., Gusmaroli G., Charron J.B., Yoon H.J., Chen H.,
RA He Y.J., Xiong Y., Deng X.W.;
RT "Characterization of Arabidopsis and rice DWD proteins and their roles as
RT substrate receptors for CUL4-RING E3 ubiquitin ligases.";
RL Plant Cell 20:152-167(2008).
RN [14]
RP FUNCTION.
RX PubMed=18045841; DOI=10.1242/dev.010108;
RA Chen Z., Tan J.L., Ingouff M., Sundaresan V., Berger F.;
RT "Chromatin assembly factor 1 regulates the cell cycle but not cell fate
RT during male gametogenesis in Arabidopsis thaliana.";
RL Development 135:65-73(2008).
RN [15]
RP INTERACTION WITH EMF1.
RX PubMed=18281509; DOI=10.1105/tpc.106.049957;
RA Calonje M., Sanchez R., Chen L., Sung Z.R.;
RT "EMBRYONIC FLOWER1 participates in polycomb group-mediated AG gene
RT silencing in Arabidopsis.";
RL Plant Cell 20:277-291(2008).
RN [16]
RP SUBUNIT, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18854416; DOI=10.1073/pnas.0808687105;
RA De Lucia F., Crevillen P., Jones A.M.E., Greb T., Dean C.;
RT "A PHD-polycomb repressive complex 2 triggers the epigenetic silencing of
RT FLC during vernalization.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:16831-16836(2008).
RN [17]
RP INTERACTION WITH ALP1.
RX PubMed=26642436; DOI=10.1371/journal.pgen.1005660;
RA Liang S.C., Hartwig B., Perera P., Mora-Garcia S., de Leau E., Thornton H.,
RA de Lima Alves F., de Alves F.L., Rappsilber J., Rapsilber J., Yang S.,
RA James G.V., Schneeberger K., Finnegan E.J., Turck F., Goodrich J.;
RT "Kicking against the PRCs - A domesticated transposase antagonises
RT silencing mediated by polycomb group proteins and is an accessory component
RT of polycomb repressive complex 2.";
RL PLoS Genet. 11:E1005660-E1005660(2015).
CC -!- FUNCTION: Core histone-binding subunit that may target chromatin
CC assembly factors, chromatin remodeling factors and histone deacetylases
CC to their histone substrates in a manner that is regulated by
CC nucleosomal DNA. Component of several complexes which regulate
CC chromatin metabolism. These include the chromatin assembly factor 1
CC (CAF-1) complex, which is required for chromatin assembly following DNA
CC replication and DNA repair, and the fertilization independent seed
CC (FIS) complex, a polycomb group protein complex which is required to
CC maintain the transcriptionally repressive state of homeotic genes
CC throughout development. Required for several aspects of plant
CC development including normal leaf expansion, correct development of
CC flowers, normal endosperm development, repression of parthenogenetic
CC seed development and repression of floral homeotic genes in leaf
CC tissue. Also required for the recruitment of chromosomal DNA into
CC heterochromatic chromocenters. Also involved in regulation of imprinted
CC genes. Acts together with RBR1 to repress the expression of MET1 during
CC female gametogenesis. This in turn activates expression of the
CC imprinted genes FIS2 and FWA. {ECO:0000269|PubMed:11163246,
CC ECO:0000269|PubMed:12736201, ECO:0000269|PubMed:12970192,
CC ECO:0000269|PubMed:15151989, ECO:0000269|PubMed:15854908,
CC ECO:0000269|PubMed:16452472, ECO:0000269|PubMed:17021044,
CC ECO:0000269|PubMed:18045841, ECO:0000269|PubMed:18700816}.
CC -!- SUBUNIT: Binds directly to helix 1 of the histone fold of histone H4, a
CC region that is not accessible when H4 is in chromatin (By similarity).
CC Component of the chromatin assembly factor 1 (CAF-1) complex, composed
CC of FAS1, FAS2 and MSI1. Component of the FIS complex which includes at
CC least MEA, FIE, MIS1 and possibly FIS2. Interacts with EMF1. Component
CC of the plant homeodomain / polycomb repressive complex 2 (PHD-PRC2)
CC large complex during prolonged cold, composed of core PRC2 components
CC (VRN2, EZA1, FIE and MSI1), and three related PHD finger proteins
CC (VIL1, VIL2 and VIN3) that mediates histone H3 trimethylation on 'Lys-
CC 27' H3K27me3. Interacts with RBR1. Binds to ALP1 (PubMed:26642436).
CC {ECO:0000250, ECO:0000269|PubMed:11163246, ECO:0000269|PubMed:12970192,
CC ECO:0000269|PubMed:18281509, ECO:0000269|PubMed:18700816,
CC ECO:0000269|PubMed:18854416, ECO:0000269|PubMed:26642436}.
CC -!- INTERACTION:
CC O22467; Q9M0V3-1: DDB1A; NbExp=3; IntAct=EBI-632891, EBI-8565056;
CC O22467; Q8L6Y4: EMF2; NbExp=4; IntAct=EBI-632891, EBI-2128696;
CC O22467; Q9LT47: FIE; NbExp=6; IntAct=EBI-632891, EBI-307146;
CC O22467; Q946J8: LHP1; NbExp=4; IntAct=EBI-632891, EBI-2309089;
CC O22467; Q9LKZ3: RBR1; NbExp=2; IntAct=EBI-632891, EBI-398590;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed. Strongly expressed in
CC floral buds and flowers, the female gametophyte and the sporophytic
CC tissue of the ovules. Also strongly expressed in developing embryos
CC following fertilization. {ECO:0000269|PubMed:12736201}.
CC -!- DEVELOPMENTAL STAGE: Expressed during female gametophyte development.
CC {ECO:0000269|PubMed:18700816}.
CC -!- DOMAIN: The DWD box is required for interaction with DDB1A.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
CC {ECO:0000305}.
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DR EMBL; AF016846; AAB70242.1; -; mRNA.
DR EMBL; AB019228; BAA96914.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97021.1; -; Genomic_DNA.
DR EMBL; AY059874; AAL24356.1; -; mRNA.
DR EMBL; AY114646; AAM47965.1; -; mRNA.
DR RefSeq; NP_200631.1; NM_125208.3.
DR AlphaFoldDB; O22467; -.
DR SMR; O22467; -.
DR BioGRID; 21179; 68.
DR DIP; DIP-33481N; -.
DR IntAct; O22467; 36.
DR MINT; O22467; -.
DR STRING; 3702.AT5G58230.1; -.
DR PaxDb; O22467; -.
DR PRIDE; O22467; -.
DR ProteomicsDB; 239004; -.
DR EnsemblPlants; AT5G58230.1; AT5G58230.1; AT5G58230.
DR GeneID; 835935; -.
DR Gramene; AT5G58230.1; AT5G58230.1; AT5G58230.
DR KEGG; ath:AT5G58230; -.
DR Araport; AT5G58230; -.
DR TAIR; locus:2161253; AT5G58230.
DR eggNOG; KOG0264; Eukaryota.
DR HOGENOM; CLU_020445_3_1_1; -.
DR InParanoid; O22467; -.
DR OMA; PHEEGCL; -.
DR OrthoDB; 831322at2759; -.
DR PhylomeDB; O22467; -.
DR PRO; PR:O22467; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; O22467; baseline and differential.
DR Genevisible; O22467; AT.
DR GO; GO:0005677; C:chromatin silencing complex; IDA:UniProtKB.
DR GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; ISS:TAIR.
DR GO; GO:0070176; C:DRM complex; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0032991; C:protein-containing complex; IPI:TAIR.
DR GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR GO; GO:0006338; P:chromatin remodeling; IBA:GO_Central.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0031507; P:heterochromatin assembly; IMP:TAIR.
DR GO; GO:0070828; P:heterochromatin organization; IMP:TAIR.
DR GO; GO:0048366; P:leaf development; IMP:TAIR.
DR GO; GO:0009555; P:pollen development; IMP:TAIR.
DR GO; GO:0045787; P:positive regulation of cell cycle; IGI:TAIR.
DR GO; GO:0009909; P:regulation of flower development; IMP:TAIR.
DR GO; GO:0006349; P:regulation of gene expression by genomic imprinting; IPI:TAIR.
DR GO; GO:2000653; P:regulation of genetic imprinting; IDA:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0010214; P:seed coat development; IMP:TAIR.
DR GO; GO:0048316; P:seed development; IMP:TAIR.
DR GO; GO:0010026; P:trichome differentiation; IMP:TAIR.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR022052; Histone-bd_RBBP4_N.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF12265; CAF1C_H4-bd; 1.
DR Pfam; PF00400; WD40; 3.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Chromatin regulator; Developmental protein; Differentiation;
KW DNA replication; Flowering; Nucleus; Reference proteome; Repeat; Repressor;
KW Transcription; Transcription regulation; WD repeat.
FT CHAIN 1..424
FT /note="Histone-binding protein MSI1"
FT /id="PRO_0000051080"
FT REPEAT 123..163
FT /note="WD 1"
FT REPEAT 176..216
FT /note="WD 2"
FT REPEAT 225..265
FT /note="WD 3"
FT REPEAT 271..311
FT /note="WD 4"
FT REPEAT 315..355
FT /note="WD 5"
FT REPEAT 372..412
FT /note="WD 6"
FT MOTIF 243..258
FT /note="DWD box"
SQ SEQUENCE 424 AA; 48195 MW; 367DF89C861ECADF CRC64;
MGKDEEEMRG EIEERLINEE YKIWKKNTPF LYDLVITHAL EWPSLTVEWL PDREEPSGKD
YSVQKMILGT HTSESEPNYL MLAQVQLPLD DTESEARQYD DDRSEFGGFG CATGKVQIIQ
QINHDGEVNR ARYMPQNPFI IATKTVNAEV YVFDYSKHPS KPPLDGACNP DLKLRGHSSE
GYGLSWSKFK QGHLLSGSDD AQICLWDINA TPKNKSLDAQ QIFKAHEGVV EDVAWHLRHE
YLFGSVGDDQ YLLIWDLRSP SASKPVQSVV AHSMEVNCLA FNPFNEWVVA TGSTDKTVKL
FDLRKLSTAL HTFDSHKEEV FQVGWNPKNE TILASCCLGR RLMVWDLSRI DEEQTVEDAE
DGPPELLFIH GGHTSKISDF SWNPCEDWVI SSVAEDNILQ IWQMAENIYH DEDDAPGEEP
SKAS
