ID   MSI2_ARATH              Reviewed;         415 AA.
AC   O22468;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 149.
DE   RecName: Full=WD-40 repeat-containing protein MSI2;
GN   Name=MSI2; OrderedLocusNames=At2g16780; ORFNames=T24I21.19;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=9338962; DOI=10.2307/3870446;
RA   Ach R.A., Taranto P., Gruissem W.;
RT   "A conserved family of WD-40 proteins binds to the retinoblastoma protein
RT   in both plants and animals.";
RL   Plant Cell 9:1595-1606(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [5]
RP   DWD MOTIF.
RX   PubMed=18223036; DOI=10.1105/tpc.107.055418;
RA   Lee J.H., Terzaghi W., Gusmaroli G., Charron J.B., Yoon H.J., Chen H.,
RA   He Y.J., Xiong Y., Deng X.W.;
RT   "Characterization of Arabidopsis and rice DWD proteins and their roles as
RT   substrate receptors for CUL4-RING E3 ubiquitin ligases.";
RL   Plant Cell 20:152-167(2008).
CC   -!- FUNCTION: Core histone-binding subunit that may target chromatin
CC       assembly factors, chromatin remodeling factors and histone deacetylases
CC       to their histone substrates in a manner that is regulated by
CC       nucleosomal DNA. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- DOMAIN: The DWD box is required for interaction with DDB1A.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF016847; AAB70243.1; -; mRNA.
DR   EMBL; AC005825; AAD24611.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC06537.1; -; Genomic_DNA.
DR   EMBL; AY056814; AAL10505.1; -; mRNA.
DR   PIR; B84544; B84544.
DR   RefSeq; NP_179269.1; NM_127230.4.
DR   AlphaFoldDB; O22468; -.
DR   SMR; O22468; -.
DR   STRING; 3702.AT2G16780.1; -.
DR   PaxDb; O22468; -.
DR   PRIDE; O22468; -.
DR   ProteomicsDB; 250783; -.
DR   EnsemblPlants; AT2G16780.1; AT2G16780.1; AT2G16780.
DR   GeneID; 816179; -.
DR   Gramene; AT2G16780.1; AT2G16780.1; AT2G16780.
DR   KEGG; ath:AT2G16780; -.
DR   Araport; AT2G16780; -.
DR   TAIR; locus:2059856; AT2G16780.
DR   eggNOG; KOG0264; Eukaryota.
DR   HOGENOM; CLU_020445_3_1_1; -.
DR   InParanoid; O22468; -.
DR   OMA; DSYLCIW; -.
DR   OrthoDB; 831322at2759; -.
DR   PhylomeDB; O22468; -.
DR   PRO; PR:O22468; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O22468; baseline and differential.
DR   Genevisible; O22468; AT.
DR   GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; ISS:TAIR.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR   GO; GO:0006338; P:chromatin remodeling; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR022052; Histone-bd_RBBP4_N.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   Pfam; PF12265; CAF1C_H4-bd; 1.
DR   Pfam; PF00400; WD40; 4.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 5.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 2.
DR   PROSITE; PS50082; WD_REPEATS_2; 5.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Chromatin regulator; Nucleus; Reference proteome; Repeat; Repressor;
KW   Transcription; Transcription regulation; WD repeat.
FT   CHAIN           1..415
FT                   /note="WD-40 repeat-containing protein MSI2"
FT                   /id="PRO_0000051081"
FT   REPEAT          166..206
FT                   /note="WD 1"
FT   REPEAT          215..255
FT                   /note="WD 2"
FT   REPEAT          258..298
FT                   /note="WD 3"
FT   REPEAT          302..342
FT                   /note="WD 4"
FT   REPEAT          361..401
FT                   /note="WD 5"
FT   MOTIF           232..248
FT                   /note="DWD box"
SQ   SEQUENCE   415 AA;  46704 MW;  DE9F99627CBD8A4E CRC64;
     MADEGKEETG MGQVEEDFSV WKKNTPFLYD LLISHPLEWP SLTVHWVPST PNPYVADSYF
     GVHKLILGTH TSGSAQDFLM VADVVTPTPN AEPGIGGANQ DPFIPKVEIR QRIRVDGEVN
     RARCMPQKPT LVGAKTSGCE VFLFDYAKHA AKSQTSECDP DLRLVGHDKE GYGLSWSPFK
     EGYLLSGSQD QKICLWDVSA TPQDKVLNAM FVYEGHESAI ADVSWHMKNE NLFGSAGEDG
     RLVIWDTRTN QMQHQVKVHE REVNYLSFNP FNEWVLATAS SDSTVALFDL RKLNAPLHVM
     SSHEGEVFQV EWDPNHETVL ASSGEDRRLM VWDLNRVGEE QLEIELDAED GPPELLFSHG
     GHKAKISDFA WNKNEPWVIA SVAEDNSLQV WQMAESIYRD EEDAEDIKED ITQQS