MSI3_ARATH
ID MSI3_ARATH Reviewed; 424 AA.
AC O22469; O49612;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2001, sequence version 2.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=WD-40 repeat-containing protein MSI3;
GN Name=MSI3; OrderedLocusNames=At4g35050; ORFNames=M4E13.110;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9338962; DOI=10.2307/3870446;
RA Ach R.A., Taranto P., Gruissem W.;
RT "A conserved family of WD-40 proteins binds to the retinoblastoma protein
RT in both plants and animals.";
RL Plant Cell 9:1595-1606(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP DWD MOTIF.
RX PubMed=18223036; DOI=10.1105/tpc.107.055418;
RA Lee J.H., Terzaghi W., Gusmaroli G., Charron J.B., Yoon H.J., Chen H.,
RA He Y.J., Xiong Y., Deng X.W.;
RT "Characterization of Arabidopsis and rice DWD proteins and their roles as
RT substrate receptors for CUL4-RING E3 ubiquitin ligases.";
RL Plant Cell 20:152-167(2008).
CC -!- FUNCTION: Core histone-binding subunit that may target chromatin
CC assembly factors, chromatin remodeling factors and histone deacetylases
CC to their histone substrates in a manner that is regulated by
CC nucleosomal DNA. {ECO:0000250}.
CC -!- INTERACTION:
CC O22469; Q9M0V3: DDB1A; NbExp=2; IntAct=EBI-1632794, EBI-1632780;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DOMAIN: The DWD box is required for interaction with DDB1A.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF016848; AAB70244.1; -; mRNA.
DR EMBL; AL022023; CAA17770.1; -; Genomic_DNA.
DR EMBL; AL161586; CAB80222.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86455.1; -; Genomic_DNA.
DR PIR; T05775; T05775.
DR RefSeq; NP_195231.1; NM_119671.3.
DR AlphaFoldDB; O22469; -.
DR SMR; O22469; -.
DR BioGRID; 14939; 2.
DR IntAct; O22469; 1.
DR STRING; 3702.AT4G35050.1; -.
DR PaxDb; O22469; -.
DR PRIDE; O22469; -.
DR ProteomicsDB; 250784; -.
DR EnsemblPlants; AT4G35050.1; AT4G35050.1; AT4G35050.
DR GeneID; 829657; -.
DR Gramene; AT4G35050.1; AT4G35050.1; AT4G35050.
DR KEGG; ath:AT4G35050; -.
DR Araport; AT4G35050; -.
DR TAIR; locus:2131551; AT4G35050.
DR eggNOG; KOG0264; Eukaryota.
DR HOGENOM; CLU_020445_3_1_1; -.
DR InParanoid; O22469; -.
DR OMA; FAWNKDE; -.
DR OrthoDB; 831322at2759; -.
DR PhylomeDB; O22469; -.
DR PRO; PR:O22469; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O22469; baseline and differential.
DR Genevisible; O22469; AT.
DR GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; IPI:TAIR.
DR GO; GO:0031010; C:ISWI-type complex; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR GO; GO:0006338; P:chromatin remodeling; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR022052; Histone-bd_RBBP4_N.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF12265; CAF1C_H4-bd; 1.
DR Pfam; PF00400; WD40; 4.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 4.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Acetylation; Chromatin regulator; Nucleus; Reference proteome; Repeat;
KW Repressor; Transcription; Transcription regulation; WD repeat.
FT CHAIN 1..424
FT /note="WD-40 repeat-containing protein MSI3"
FT /id="PRO_0000051082"
FT REPEAT 167..207
FT /note="WD 1"
FT REPEAT 216..256
FT /note="WD 2"
FT REPEAT 259..299
FT /note="WD 3"
FT REPEAT 303..343
FT /note="WD 4"
FT REPEAT 362..402
FT /note="WD 5"
FT REGION 394..424
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 233..249
FT /note="DWD box"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:O22607"
FT CONFLICT 223..224
FT /note="DV -> EL (in Ref. 1; AAB70244)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 424 AA; 47983 MW; D83E2B4913468A0A CRC64;
MAAEEGKDEA GLDQVEEEFS IWKRNTPFLY DLMISHPLEW PSLTLHWVPS TPIPYSKDPY
FAVHKLILGT HTSGGAQDFL MVADVVIPTP DAEPGLGGRD QEPIVPKVEI KQKIRVDGEV
NRARCMPQKP TLVGAKTSGS EVFLFDYARL SGKPQTSECD PDLRLMGHEQ EGYGLAWSSF
KEGYLLSGSQ DQRICLWDVS ATATDKVLNP MHVYEGHQSI IEDVAWHMKN ENIFGSAGDD
CQLVIWDLRT NQMQHQVKVH EREINYLSFN PFNEWVLATA SSDSTVALFD LRKLTAPLHV
LSKHEGEVFQ VEWDPNHETV LASSGEDRRL MVWDINRVGD EQLEIELDAE DGPPELLFSH
GGHKAKISDF AWNKDEPWVI SSVAEDNSLQ VWQMAESIYR EDDEDEDDDD EGNQNAQHSN
ENQK
