MSIR6_DROME
ID MSIR6_DROME Reviewed; 369 AA.
AC Q9VVE5; A8JNU7; A8JNU8; Q26276; Q8MQZ1;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 3.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=RNA-binding protein Musashi homolog Rbp6 {ECO:0000250|UniProtKB:Q920Q6, ECO:0000303|PubMed:8417324};
GN Name=Rbp6 {ECO:0000303|PubMed:8417324};
GN Synonyms=RRM6 {ECO:0000303|PubMed:8417324}; ORFNames=CG32169;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|EMBL:ABW08559.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305, ECO:0000312|EMBL:ABW08559.1}
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAM52738.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM52738.1};
RC TISSUE=Embryo {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305, ECO:0000312|EMBL:AAB24627.1}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 32-75 (ISOFORMS A/B).
RX PubMed=8417324; DOI=10.1128/mcb.13.1.174-183.1993;
RA Kim Y.-J., Baker B.S.;
RT "Isolation of RRM-type RNA-binding protein genes and the analysis of their
RT relatedness by using a numerical approach.";
RL Mol. Cell. Biol. 13:174-183(1993).
CC -!- FUNCTION: RNA binding protein that regulates the expression of target
CC mRNAs at the translation level. May play a role in the proliferation
CC and maintenance of stem cells in the central nervous system (By
CC similarity). {ECO:0000250|UniProtKB:Q920Q6}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q920Q6}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=A {ECO:0000269|PubMed:10731132};
CC IsoId=Q9VVE5-1; Sequence=Displayed;
CC Name=B {ECO:0000269|PubMed:10731132};
CC IsoId=Q9VVE5-2; Sequence=VSP_053087;
CC Name=C {ECO:0000269|PubMed:10731132};
CC IsoId=Q9VVE5-3; Sequence=VSP_053086;
CC -!- SIMILARITY: Belongs to the Musashi family. {ECO:0000305}.
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DR EMBL; AE014296; AAF49366.3; -; Genomic_DNA.
DR EMBL; AE014296; ABW08559.1; -; Genomic_DNA.
DR EMBL; AE014296; ABW08560.1; -; Genomic_DNA.
DR EMBL; AY122226; AAM52738.1; -; mRNA.
DR EMBL; S51715; AAB24627.1; -; mRNA.
DR PIR; F48110; F48110.
DR RefSeq; NP_001097631.1; NM_001104161.3. [Q9VVE5-2]
DR RefSeq; NP_001097632.1; NM_001104162.4. [Q9VVE5-3]
DR RefSeq; NP_730245.2; NM_168718.3. [Q9VVE5-1]
DR AlphaFoldDB; Q9VVE5; -.
DR SMR; Q9VVE5; -.
DR BioGRID; 65211; 26.
DR DIP; DIP-19056N; -.
DR IntAct; Q9VVE5; 24.
DR STRING; 7227.FBpp0112339; -.
DR PaxDb; Q9VVE5; -.
DR DNASU; 39919; -.
DR EnsemblMetazoa; FBtr0075285; FBpp0075045; FBgn0260943. [Q9VVE5-1]
DR EnsemblMetazoa; FBtr0113427; FBpp0112339; FBgn0260943. [Q9VVE5-2]
DR EnsemblMetazoa; FBtr0113428; FBpp0112340; FBgn0260943. [Q9VVE5-3]
DR GeneID; 39919; -.
DR KEGG; dme:Dmel_CG32169; -.
DR UCSC; CG32169-RA; d. melanogaster. [Q9VVE5-1]
DR UCSC; CG32169-RB; d. melanogaster.
DR UCSC; CG32169-RC; d. melanogaster.
DR CTD; 39919; -.
DR FlyBase; FBgn0260943; Rbp6.
DR VEuPathDB; VectorBase:FBgn0260943; -.
DR eggNOG; KOG4205; Eukaryota.
DR GeneTree; ENSGT00940000155420; -.
DR InParanoid; Q9VVE5; -.
DR PhylomeDB; Q9VVE5; -.
DR Reactome; R-DME-9013418; RHOBTB2 GTPase cycle.
DR SignaLink; Q9VVE5; -.
DR BioGRID-ORCS; 39919; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 39919; -.
DR PRO; PR:Q9VVE5; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0260943; Expressed in brain and 24 other tissues.
DR ExpressionAtlas; Q9VVE5; baseline and differential.
DR Genevisible; Q9VVE5; DM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005844; C:polysome; ISS:UniProtKB.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0008266; F:poly(U) RNA binding; ISS:UniProtKB.
DR GO; GO:0003727; F:single-stranded RNA binding; ISS:UniProtKB.
DR GO; GO:0006417; P:regulation of translation; IBA:GO_Central.
DR GO; GO:0048864; P:stem cell development; ISS:UniProtKB.
DR CDD; cd12576; RRM1_MSI; 1.
DR CDD; cd12323; RRM2_MSI; 1.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR034130; MSI_RRM1.
DR InterPro; IPR034126; MSI_RRM2.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 2.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50102; RRM; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..369
FT /note="RNA-binding protein Musashi homolog Rbp6"
FT /id="PRO_0000374051"
FT DOMAIN 29..108
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 118..195
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 298..319
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..112
FT /note="Missing (in isoform C)"
FT /evidence="ECO:0000303|PubMed:10731132"
FT /id="VSP_053086"
FT VAR_SEQ 216..369
FT /note="DNIMWGLGTLPEGFPAAAYAAYAAGRGYSGYPSFGLPYPTVDLTNGQLTPNN
FT NTPLLTQALSVMNNYQAAAAAAHSYGPSASPHAAAANTATRQGFPSTNSPGPTIDMYSS
FT AAADNVGYVQATSPQPSGFPIAVSRAPLNYSPIYSLNFAMANI -> GELVVWGSSHAH
FT STAATSAAAAGLLPSSLAAAASVLQQQQQQQQQQQQQQQQQQQHHQQLHHTHPQSPAHS
FT HAHHPHTHSHSQLLGSLRYTPYPLPAHLSAAAVVVAQQQHHHQQQQQQQQQQQQQQVVA
FT AQQQHQQQQSLAQVVAAAAGAAPGLLPLANPAPPATPSLLQFAAGQSNAALANSLYADA
FT AAVVGYKRLLAAAAVSSGLRAPTTALGALQAAAANVPAAAAAAQLQQAQLRQNAALAAA
FT HYPLSELLAMQGGMEMGAGANSAAAAAASLYQLPGI (in isoform B)"
FT /evidence="ECO:0000303|PubMed:10731132"
FT /id="VSP_053087"
FT CONFLICT 33
FT /note="I -> V (in Ref. 4; AAM52738)"
FT /evidence="ECO:0000305"
FT CONFLICT 73
FT /note="G -> A (in Ref. 4; AAM52738)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 369 AA; 40087 MW; ADDA310C4CF993C4 CRC64;
MDIKIEQQQQ QQQVELGPCS PSEVPNDPGK MFIGGLSWQT SPESLRDYFG RYGDISEAMV
MKDPTTRRSR GFGFVTFSDP NSVDKVLTQG THELDGKKVD PKVAFPRRAH PKMVTRTKKI
FVGGLSAPTT LEDVKSYFEQ FGPIEDAMLM FDKQTNRHRG FGFVTFQSED VVDKVCEIHF
HEINNKMVEC KKAQPKEVML PANLAKTRAA GRSAYDNIMW GLGTLPEGFP AAAYAAYAAG
RGYSGYPSFG LPYPTVDLTN GQLTPNNNTP LLTQALSVMN NYQAAAAAAH SYGPSASPHA
AAANTATRQG FPSTNSPGPT IDMYSSAAAD NVGYVQATSP QPSGFPIAVS RAPLNYSPIY
SLNFAMANI
