ID   MSK1_TOBAC              Reviewed;         409 AA.
AC   Q40518;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Shaggy-related protein kinase NtK-1;
DE            EC=2.7.11.1;
GN   Name=NTK-1;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=7873606; DOI=10.1016/0167-4781(94)00229-v;
RA   Einzenberger E., Eller N., Heberle-Bors E., Vicente O.;
RT   "Isolation and expression during pollen development of a tobacco cDNA clone
RT   encoding a protein kinase homologous to shaggy/glycogen synthase kinase-
RT   3.";
RL   Biochim. Biophys. Acta 1260:315-319(1995).
CC   -!- FUNCTION: May mediate extracellular signals to regulate transcription
CC       in differentiating cells. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC         [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC         threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC         Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC         EC=2.7.11.1;
CC   -!- PTM: Autophosphorylated mainly on threonine and serine residues.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr
CC       protein kinase family. GSK-3 subfamily. {ECO:0000305}.
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DR   EMBL; X77763; CAA54803.1; -; mRNA.
DR   PIR; S52095; S52095.
DR   RefSeq; NP_001312250.1; NM_001325321.1.
DR   AlphaFoldDB; Q40518; -.
DR   SMR; Q40518; -.
DR   GeneID; 107781719; -.
DR   KEGG; nta:107781719; -.
DR   BRENDA; 2.7.11.26; 3645.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR   GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR   GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR   GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR   CDD; cd14137; STKc_GSK3; 1.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR000719; Prot_kinase_dom.
DR   InterPro; IPR017441; Protein_kinase_ATP_BS.
DR   InterPro; IPR008271; Ser/Thr_kinase_AS.
DR   InterPro; IPR039192; STKc_GSK3.
DR   Pfam; PF00069; Pkinase; 1.
DR   SMART; SM00220; S_TKc; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR   PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Kinase; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Serine/threonine-protein kinase; Transferase.
FT   CHAIN           1..409
FT                   /note="Shaggy-related protein kinase NtK-1"
FT                   /id="PRO_0000086403"
FT   DOMAIN          73..357
FT                   /note="Protein kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   REGION          1..27
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..17
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        198
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT                   ECO:0000255|PROSITE-ProRule:PRU10027"
FT   BINDING         79..87
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   BINDING         102
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ   SEQUENCE   409 AA;  46309 MW;  A6D4E1ED2AF1265C CRC64;
     MTSVGLAPVS GLRESSSHSV GVDRLPEEMN DMRIRDDKEI EAAIVDGNGT ETGHIIVTTI
     GGRHGQPKQT ISYMAERIVG QGSFGVVFQA KCLETGETVA IKKVLQDKRY KNRELQTMRL
     LDHPNVVCLK HCFFSTTEKD EVYLNLVLEY VPETVHRVIK HYNKLNQRMP LILVKLYTYQ
     IFRALSYIHH TIGVCHRDIK PQNLLVNPHT HQVKLCDFGS AKVLVKGEPN ISYICSRYYR
     APELIFGATE YTTAIDIWSA GCVLAELLLG QPLFPGESGV DQLVEIIKVL GTPTREEIKC
     MNPNYNEFKF PQIKAHPWHK IFHKRMPPEA VDLVSRLLQY SPNLRCTALE AVTHAFFDEL
     RDPNTRLPNG RVLPPLFNFK AHELKGVSAE NLLKLVPEHA RKQCPSLGL