MSL1_YEAST
ID MSL1_YEAST Reviewed; 111 AA.
AC P40567; D6VVT9;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=U2 small nuclear ribonucleoprotein B'';
DE Short=U2 snRNP B'';
GN Name=MSL1; OrderedLocusNames=YIR009W; ORFNames=YIB9W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7762303; DOI=10.1002/yea.320110109;
RA Voss H., Tamames J., Teodoru C., Valencia A., Sensen C., Wiemann S.,
RA Schwager C., Zimmermann J., Sander C., Ansorge W.;
RT "Nucleotide sequence and analysis of the centromeric region of yeast
RT chromosome IX.";
RL Yeast 11:61-78(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP FUNCTION.
RX PubMed=8649387; DOI=10.1128/mcb.16.6.2787;
RA Tang J., Abovich N., Rosbash M.;
RT "Identification and characterization of a yeast gene encoding the U2 small
RT nuclear ribonucleoprotein particle B' protein.";
RL Mol. Cell. Biol. 16:2787-2795(1996).
RN [6]
RP INTERACTION WITH LEA1.
RX PubMed=9799242; DOI=10.1093/emboj/17.21.6348;
RA Caspary F., Seraphin B.;
RT "The yeast U2A'/U2B complex is required for pre-spliceosome formation.";
RL EMBO J. 17:6348-6358(1998).
RN [7]
RP IDENTIFICATION IN THE CWC COMPLEX, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11884590; DOI=10.1128/mcb.22.7.2011-2024.2002;
RA Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.;
RT "Proteomics analysis reveals stable multiprotein complexes in both fission
RT and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA
RT splicing factors, and snRNAs.";
RL Mol. Cell. Biol. 22:2011-2024(2002).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Involved in pre-mRNA splicing. This protein is associated
CC with snRNP U2. It binds stem loop IV of U2 snRNA.
CC {ECO:0000269|PubMed:8649387}.
CC -!- SUBUNIT: Belongs to the CWC complex (or CEF1-associated complex), a
CC spliceosome sub-complex reminiscent of a late-stage spliceosome
CC composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2,
CC CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24,
CC CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9,
CC PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3,
CC SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts
CC with RDS3. Interacts with LEA1. {ECO:0000269|PubMed:11884590,
CC ECO:0000269|PubMed:9799242}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- MISCELLANEOUS: Present with 504 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; X79743; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; Z37996; CAA86079.1; -; Genomic_DNA.
DR EMBL; AY558237; AAS56563.1; -; Genomic_DNA.
DR EMBL; BK006942; DAA08555.1; -; Genomic_DNA.
DR PIR; S48353; S48353.
DR RefSeq; NP_012274.1; NM_001179531.1.
DR PDB; 5GMK; EM; 3.40 A; a=1-111.
DR PDB; 5LJ3; EM; 3.80 A; Y=1-111.
DR PDB; 5LJ5; EM; 3.80 A; Y=1-111.
DR PDB; 5MQ0; EM; 4.17 A; Y=1-111.
DR PDB; 5NRL; EM; 7.20 A; Y=1-111.
DR PDB; 5WSG; EM; 4.00 A; X=1-111.
DR PDB; 5Y88; EM; 3.70 A; p=1-111.
DR PDB; 5YLZ; EM; 3.60 A; p=1-111.
DR PDB; 5ZWM; EM; 3.40 A; p=1-111.
DR PDB; 5ZWO; EM; 3.90 A; p=1-111.
DR PDB; 6BK8; EM; 3.30 A; r=1-111.
DR PDB; 6EXN; EM; 3.70 A; Y=1-111.
DR PDB; 6G90; EM; 4.00 A; Y=1-111.
DR PDB; 6J6G; EM; 3.20 A; a=1-111.
DR PDB; 6J6H; EM; 3.60 A; a=1-111.
DR PDB; 6J6N; EM; 3.86 A; a=1-111.
DR PDB; 6J6Q; EM; 3.70 A; a=1-111.
DR PDB; 7OQB; EM; 9.00 A; Y=1-111.
DR PDB; 7OQE; EM; 5.90 A; Y=1-111.
DR PDBsum; 5GMK; -.
DR PDBsum; 5LJ3; -.
DR PDBsum; 5LJ5; -.
DR PDBsum; 5MQ0; -.
DR PDBsum; 5NRL; -.
DR PDBsum; 5WSG; -.
DR PDBsum; 5Y88; -.
DR PDBsum; 5YLZ; -.
DR PDBsum; 5ZWM; -.
DR PDBsum; 5ZWO; -.
DR PDBsum; 6BK8; -.
DR PDBsum; 6EXN; -.
DR PDBsum; 6G90; -.
DR PDBsum; 6J6G; -.
DR PDBsum; 6J6H; -.
DR PDBsum; 6J6N; -.
DR PDBsum; 6J6Q; -.
DR PDBsum; 7OQB; -.
DR PDBsum; 7OQE; -.
DR AlphaFoldDB; P40567; -.
DR SMR; P40567; -.
DR BioGRID; 35001; 86.
DR ComplexPortal; CPX-1651; PRP19-associated complex.
DR ComplexPortal; CPX-26; U2 small nuclear ribonucleoprotein complex.
DR DIP; DIP-816N; -.
DR IntAct; P40567; 12.
DR MINT; P40567; -.
DR STRING; 4932.YIR009W; -.
DR MaxQB; P40567; -.
DR PaxDb; P40567; -.
DR PRIDE; P40567; -.
DR EnsemblFungi; YIR009W_mRNA; YIR009W; YIR009W.
DR GeneID; 854826; -.
DR KEGG; sce:YIR009W; -.
DR SGD; S000001448; MSL1.
DR VEuPathDB; FungiDB:YIR009W; -.
DR eggNOG; KOG4206; Eukaryota.
DR GeneTree; ENSGT00390000007046; -.
DR HOGENOM; CLU_041869_3_2_1; -.
DR OMA; EYSHKDS; -.
DR BioCyc; YEAST:G3O-31430-MON; -.
DR PRO; PR:P40567; -.
DR Proteomes; UP000002311; Chromosome IX.
DR RNAct; P40567; protein.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0005681; C:spliceosomal complex; IC:ComplexPortal.
DR GO; GO:0005685; C:U1 snRNP; IBA:GO_Central.
DR GO; GO:0005686; C:U2 snRNP; IC:ComplexPortal.
DR GO; GO:0071004; C:U2-type prespliceosome; IDA:SGD.
DR GO; GO:0030619; F:U1 snRNA binding; IBA:GO_Central.
DR GO; GO:0030620; F:U2 snRNA binding; IPI:SGD.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IMP:SGD.
DR GO; GO:1903241; P:U2-type prespliceosome assembly; IC:ComplexPortal.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 1: Evidence at protein level;
KW 3D-structure; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Ribonucleoprotein; RNA-binding.
FT CHAIN 1..111
FT /note="U2 small nuclear ribonucleoprotein B''"
FT /id="PRO_0000081895"
FT DOMAIN 28..106
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT STRAND 30..32
FT /evidence="ECO:0007829|PDB:6J6G"
FT STRAND 37..39
FT /evidence="ECO:0007829|PDB:6J6G"
FT HELIX 41..52
FT /evidence="ECO:0007829|PDB:6J6G"
FT TURN 53..55
FT /evidence="ECO:0007829|PDB:6J6G"
FT STRAND 58..62
FT /evidence="ECO:0007829|PDB:6J6G"
FT TURN 66..70
FT /evidence="ECO:0007829|PDB:5GMK"
FT STRAND 71..77
FT /evidence="ECO:0007829|PDB:6J6G"
FT HELIX 79..88
FT /evidence="ECO:0007829|PDB:6J6G"
FT TURN 89..91
FT /evidence="ECO:0007829|PDB:6J6G"
FT STRAND 100..103
FT /evidence="ECO:0007829|PDB:5GMK"
SQ SEQUENCE 111 AA; 12830 MW; 8FB0974FC30994AE CRC64;
MVEPARKKQR IDRDTHHTVA EPVTEAKNTL YVSQLNEKIN MQRLRVNLFL LFATFGEVLK
VSMNFKKQRG QAFITMRTID QASLAQISLN GERFFGKPLK VEFSKSETKT L
