MSL2_ARATH
ID MSL2_ARATH Reviewed; 673 AA.
AC Q56X46; B9DHF7; F4KGW2; Q9LXA1;
DT 25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Mechanosensitive ion channel protein 2, chloroplastic;
DE AltName: Full=Mechanosensitive channel of small conductance-like 2;
DE AltName: Full=MscS-Like protein 2;
DE Flags: Precursor;
GN Name=MSL2; OrderedLocusNames=At5g10490; ORFNames=F12B17.160;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP GENE FAMILY.
RX PubMed=12626684; DOI=10.1128/mmbr.67.1.66-85.2003;
RA Pivetti C.D., Yen M.R., Miller S., Busch W., Tseng Y.H., Booth I.R.,
RA Saier M.H. Jr.;
RT "Two families of mechanosensitive channel proteins.";
RL Microbiol. Mol. Biol. Rev. 67:66-85(2003).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=16401419; DOI=10.1016/j.cub.2005.11.044;
RA Haswell E.S., Meyerowitz E.M.;
RT "MscS-like proteins control plastid size and shape in Arabidopsis
RT thaliana.";
RL Curr. Biol. 16:1-11(2006).
RN [7]
RP REVIEW, GENE FAMILY, AND NOMENCLATURE.
RX DOI=10.1016/S1063-5823(06)58013-5;
RA Haswell E.S.;
RT "MscS-like proteins in plants.";
RL (In) Hamill O.P. (eds.);
RL Mechanosensitive Ion Channels, Part A, pp.329-349, Academic Press, San
RL Diego. (2007).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=21810996; DOI=10.1105/tpc.111.088112;
RA Wilson M.E., Jensen G.S., Haswell E.S.;
RT "Two mechanosensitive channel homologs influence division ring placement in
RT Arabidopsis chloroplasts.";
RL Plant Cell 23:2939-2949(2011).
CC -!- FUNCTION: Mechanosensitive channel that opens in response to stretch
CC forces in the membrane lipid bilayer (By similarity). Controls plastid
CC size, shape, and perhaps division during normal plant development by
CC altering ion flux in response to changes in membrane tension. Acts as a
CC component of the chloroplast division machinery. {ECO:0000250,
CC ECO:0000269|PubMed:16401419, ECO:0000269|PubMed:21810996}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000269|PubMed:16401419}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:16401419}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q56X46-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q56X46-2; Sequence=VSP_042196;
CC Name=3;
CC IsoId=Q56X46-3; Sequence=VSP_042195;
CC -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:16401419}.
CC -!- DISRUPTION PHENOTYPE: Msl2 and msl3 double mutant shows abnormalities
CC in the size and shape of plastids with enlarged chloroplasts containing
CC multiple FtsZ rings. {ECO:0000269|PubMed:16401419,
CC ECO:0000269|PubMed:21810996}.
CC -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB89394.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL353995; CAB89394.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED91551.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91552.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91553.1; -; Genomic_DNA.
DR EMBL; AK221831; BAD94053.1; -; mRNA.
DR EMBL; AK230015; BAF01838.1; -; mRNA.
DR EMBL; AK317509; BAH20174.1; -; mRNA.
DR PIR; T49990; T49990.
DR RefSeq; NP_001078567.1; NM_001085098.2. [Q56X46-3]
DR RefSeq; NP_001190278.1; NM_001203349.1. [Q56X46-2]
DR RefSeq; NP_568230.2; NM_121087.3. [Q56X46-1]
DR AlphaFoldDB; Q56X46; -.
DR SMR; Q56X46; -.
DR STRING; 3702.AT5G10490.1; -.
DR TCDB; 1.A.23.4.4; the small conductance mechanosensitive ion channel (mscs) family.
DR PaxDb; Q56X46; -.
DR PRIDE; Q56X46; -.
DR ProteomicsDB; 250958; -. [Q56X46-1]
DR EnsemblPlants; AT5G10490.1; AT5G10490.1; AT5G10490. [Q56X46-1]
DR EnsemblPlants; AT5G10490.2; AT5G10490.2; AT5G10490. [Q56X46-3]
DR EnsemblPlants; AT5G10490.3; AT5G10490.3; AT5G10490. [Q56X46-2]
DR GeneID; 830913; -.
DR Gramene; AT5G10490.1; AT5G10490.1; AT5G10490. [Q56X46-1]
DR Gramene; AT5G10490.2; AT5G10490.2; AT5G10490. [Q56X46-3]
DR Gramene; AT5G10490.3; AT5G10490.3; AT5G10490. [Q56X46-2]
DR KEGG; ath:AT5G10490; -.
DR Araport; AT5G10490; -.
DR TAIR; locus:2142414; AT5G10490.
DR eggNOG; ENOG502QTPM; Eukaryota.
DR InParanoid; Q56X46; -.
DR OMA; WGLAPLM; -.
DR OrthoDB; 1186886at2759; -.
DR PhylomeDB; Q56X46; -.
DR PRO; PR:Q56X46; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q56X46; baseline and differential.
DR Genevisible; Q56X46; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009526; C:plastid envelope; IDA:TAIR.
DR GO; GO:0010020; P:chloroplast fission; IGI:TAIR.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR Gene3D; 2.30.30.60; -; 1.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR006685; MscS_channel.
DR InterPro; IPR023408; MscS_dom_sf.
DR InterPro; IPR045042; YnaI-like.
DR PANTHER; PTHR43634; PTHR43634; 2.
DR Pfam; PF00924; MS_channel; 1.
DR SUPFAM; SSF50182; SSF50182; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; Ion channel; Ion transport; Membrane;
KW Phosphoprotein; Plastid; Reference proteome; Transit peptide;
KW Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..75
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 76..673
FT /note="Mechanosensitive ion channel protein 2,
FT chloroplastic"
FT /id="PRO_0000415325"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 492..673
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 505..528
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 568..587
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 588..606
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 620..641
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 656..673
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 571
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8L7W1"
FT VAR_SEQ 1..28
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_042195"
FT VAR_SEQ 2..8
FT /note="ALYGTLQ -> RNFNF (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_042196"
SQ SEQUENCE 673 AA; 74429 MW; A8F1DCCD9353C6AD CRC64;
MALYGTLQLS HSLGLCRNQR FCNPENSAMR RRLHISNGPL SLGVPLGQHG FSNILLSNYL
RRPICSVPCR TTAFRCHSFS ASGKAIEPAV KAVTVVLTKS HGLMQQFPFV YKLVPAVALL
VFSLWGLVPF ARQGRNILLN KNDNGWKKSG TYHVMTSYVQ PLLLWLGALF ICRALDPVVL
PTEASKIVKD RLLNFVRSLS TVLAFAYCLS SLIQQTQKLF SETSNPSDTR NMGFQFAGKA
LYSAVWVAAV SLFMELLGFS TQKWLTAGGL GTVLITLAGR EILTNFLSSV MIHATRPFVL
NEWIQTKIEG YEVSGTVEHV GWWSPTIIRG EDREAIHIPN HKFTVNVVRN LTQKTHWRIK
THLAISHLDV NKINNIVADM RKVLAKNPMV EQQRLHRRVF LENVIPENQA LSILISCFVK
TSHHEEYLGV KEAILLDLLR VISHHRARLA TPIRTIRKMY TETDVENTPF GESMYGGVTS
RRPLMLIEPA YKINGEDKSK SQNRAAKPTA EQENKGSNPK SKETSSPDLK ANVKVGESPV
SDTNKVPEET VAKPVIKAVS KPPTPKDTET SGTEKPKAKR SGGTIKSTKT DETDSSTSSA
SRSTLEENIV LGVALEGSKR TLPIEEEIHS PPMETDAKEL TGARRSGGNG PLVADKEQKD
SQSQPNSGAS TEP
