MSL4_ARATH
ID MSL4_ARATH Reviewed; 881 AA.
AC Q9LPG3; Q9C8K9;
DT 25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 113.
DE RecName: Full=Mechanosensitive ion channel protein 4;
DE AltName: Full=Mechanosensitive channel of small conductance-like 4;
DE AltName: Full=MscS-Like protein 4;
GN Name=MSL4; OrderedLocusNames=At1g53470; ORFNames=F22G10.15, T3F20.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=12626684; DOI=10.1128/mmbr.67.1.66-85.2003;
RA Pivetti C.D., Yen M.R., Miller S., Busch W., Tseng Y.H., Booth I.R.,
RA Saier M.H. Jr.;
RT "Two families of mechanosensitive channel proteins.";
RL Microbiol. Mol. Biol. Rev. 67:66-85(2003).
RN [4]
RP REVIEW, GENE FAMILY, AND NOMENCLATURE.
RX DOI=10.1016/S1063-5823(06)58013-5;
RA Haswell E.S.;
RT "MscS-like proteins in plants.";
RL (In) Hamill O.P. (eds.);
RL Mechanosensitive Ion Channels, Part A, pp.329-349, Academic Press, San
RL Diego. (2007).
CC -!- FUNCTION: Mechanosensitive channel that opens in response to stretch
CC forces in the membrane lipid bilayer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family. {ECO:0000305}.
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DR EMBL; AC018748; AAF78442.1; -; Genomic_DNA.
DR EMBL; AC024260; AAG51988.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32945.1; -; Genomic_DNA.
DR PIR; G96574; G96574.
DR RefSeq; NP_175752.1; NM_104225.2.
DR AlphaFoldDB; Q9LPG3; -.
DR SMR; Q9LPG3; -.
DR STRING; 3702.AT1G53470.1; -.
DR TCDB; 1.A.23.4.15; the small conductance mechanosensitive ion channel (mscs) family.
DR iPTMnet; Q9LPG3; -.
DR PaxDb; Q9LPG3; -.
DR PRIDE; Q9LPG3; -.
DR ProteomicsDB; 250789; -.
DR EnsemblPlants; AT1G53470.1; AT1G53470.1; AT1G53470.
DR GeneID; 841782; -.
DR Gramene; AT1G53470.1; AT1G53470.1; AT1G53470.
DR KEGG; ath:AT1G53470; -.
DR Araport; AT1G53470; -.
DR TAIR; locus:2024927; AT1G53470.
DR eggNOG; KOG4629; Eukaryota.
DR HOGENOM; CLU_013552_0_0_1; -.
DR InParanoid; Q9LPG3; -.
DR OMA; NRNISAW; -.
DR OrthoDB; 609350at2759; -.
DR PhylomeDB; Q9LPG3; -.
DR PRO; PR:Q9LPG3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LPG3; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0008381; F:mechanosensitive ion channel activity; IBA:GO_Central.
DR GO; GO:0006820; P:anion transport; IBA:GO_Central.
DR Gene3D; 2.30.30.60; -; 1.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR016688; MscS-like_plants/fungi.
DR InterPro; IPR006685; MscS_channel.
DR InterPro; IPR023408; MscS_dom_sf.
DR PANTHER; PTHR31618; PTHR31618; 1.
DR Pfam; PF00924; MS_channel; 1.
DR PIRSF; PIRSF017209; Memb_At2g17000_prd; 1.
DR SUPFAM; SSF50182; SSF50182; 1.
PE 3: Inferred from homology;
KW Ion channel; Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..881
FT /note="Mechanosensitive ion channel protein 4"
FT /id="PRO_0000415327"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 339..359
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 643..663
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 678..698
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 35..245
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 457..501
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 42..69
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 101..127
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 150..164
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 165..181
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 202..228
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 464..486
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 881 AA; 100415 MW; 4731869F3779095C CRC64;
MAVDSTDQRR DFVVRIDGED NGDSEKFWRE SSINFWHNDK SSKPPGGEED DGSFDFMRRS
SEKSEEPDPP SKLINQFLNK QKASGDEISL DMEANMPELQ KNTVPPLSST AVSGSASPVT
APVTASYRNG TGDAIRRRQN RVTLSPSVKD GDSSEDEENR VDGSEVVKCT SNRSTMRTKT
LMKMKTRSRL MDPPTPTYPD MVSGRTPRSG NLNPGFSGRN TKPGTPNQGG SKDLEEEEDP
FSEEDLPEGL RKEKICVWVI IEWIFLILII ASLICSLVIP YLRGKTLWDL ALWKWEVMVL
VLICGRLVSS WIVKLFVYFV ESNFLWRKKV LYFVYGIRKP VQNCLWLGLV LIAWHFLFDK
KVEREMRSTV LKYVTKVLIC LLVAVIIWLI KTLLVKVLAS SFHMSTYFDR IQESLFTQYV
IETLSGPPRI EIHIEEEKVA NDVKTFEIVG RKLSPLGPKA VSSPPQVTVG SGRLQKSPSR
VGKSPVLSRS GSKKEGGEEG IRIDHLQRMN TKNVSAWKMK KLMNVIKKGT LSTLDEQIQD
TTTQEDDKAT QIRSEFEAKL AARKIFQNVA EPGSRYIYME DFMRFLSEDE SERAMDLFEG
ASECHKISKS CLKNWVVNAF RERRALALTL NDTKTAVNRL HRIVDVLVSI VILIIWLLIL
GIATTKFLLV ISSQLLLVVF VFGNSCKTIF EAVIFVFVMH PFDVGDRCEI DGVQMIVEEM
NILTTVFLRF DNQKIVYPNS LLGTKPIANY YRSPDMQDAI EFFVHIATPP EKTTALRQRI
LSYVDNKKDH WHPSPMIVFR DMCGLNSVKI AMWPTHKMNH QNMGERYVRR GQLLEEIGRL
CRELDIEYRL YPLNINVKSL PAATPITSDR IPPSWNQQRS V
