MSL5_ARATH
ID MSL5_ARATH Reviewed; 881 AA.
AC Q9LH74; Q56YB1;
DT 25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Mechanosensitive ion channel protein 5;
DE AltName: Full=Mechanosensitive channel of small conductance-like 5;
DE AltName: Full=MscS-Like protein 5;
GN Name=MSL5; OrderedLocusNames=At3g14810; ORFNames=T21E2.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-318.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=12626684; DOI=10.1128/mmbr.67.1.66-85.2003;
RA Pivetti C.D., Yen M.R., Miller S., Busch W., Tseng Y.H., Booth I.R.,
RA Saier M.H. Jr.;
RT "Two families of mechanosensitive channel proteins.";
RL Microbiol. Mol. Biol. Rev. 67:66-85(2003).
RN [5]
RP REVIEW, GENE FAMILY, AND NOMENCLATURE.
RX DOI=10.1016/S1063-5823(06)58013-5;
RA Haswell E.S.;
RT "MscS-like proteins in plants.";
RL (In) Hamill O.P. (eds.);
RL Mechanosensitive Ion Channels, Part A, pp.329-349, Academic Press, San
RL Diego. (2007).
CC -!- FUNCTION: Mechanosensitive channel that opens in response to stretch
CC forces in the membrane lipid bilayer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9LH74-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family. {ECO:0000305}.
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DR EMBL; AP002061; BAB02647.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75568.1; -; Genomic_DNA.
DR EMBL; AK221412; BAD94388.1; -; mRNA.
DR RefSeq; NP_188099.2; NM_112342.4. [Q9LH74-1]
DR AlphaFoldDB; Q9LH74; -.
DR SMR; Q9LH74; -.
DR STRING; 3702.AT3G14810.1; -.
DR TCDB; 1.A.23.4.8; the small conductance mechanosensitive ion channel (mscs) family.
DR iPTMnet; Q9LH74; -.
DR PaxDb; Q9LH74; -.
DR PRIDE; Q9LH74; -.
DR ProteomicsDB; 239005; -. [Q9LH74-1]
DR EnsemblPlants; AT3G14810.1; AT3G14810.1; AT3G14810. [Q9LH74-1]
DR GeneID; 820710; -.
DR Gramene; AT3G14810.1; AT3G14810.1; AT3G14810. [Q9LH74-1]
DR KEGG; ath:AT3G14810; -.
DR Araport; AT3G14810; -.
DR TAIR; locus:2099382; AT3G14810.
DR eggNOG; KOG4629; Eukaryota.
DR HOGENOM; CLU_013552_0_0_1; -.
DR InParanoid; Q9LH74; -.
DR OMA; GSRYIHM; -.
DR PhylomeDB; Q9LH74; -.
DR PRO; PR:Q9LH74; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LH74; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0008381; F:mechanosensitive ion channel activity; IBA:GO_Central.
DR GO; GO:0006820; P:anion transport; IBA:GO_Central.
DR Gene3D; 2.30.30.60; -; 1.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR016688; MscS-like_plants/fungi.
DR InterPro; IPR006685; MscS_channel.
DR InterPro; IPR023408; MscS_dom_sf.
DR PANTHER; PTHR31618; PTHR31618; 1.
DR Pfam; PF00924; MS_channel; 1.
DR PIRSF; PIRSF017209; Memb_At2g17000_prd; 1.
DR SUPFAM; SSF50182; SSF50182; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Ion channel; Ion transport; Membrane; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..881
FT /note="Mechanosensitive ion channel protein 5"
FT /id="PRO_0000415328"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 309..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 349..369
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 642..662
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 677..697
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..248
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 861..881
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..38
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 44..83
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 112..146
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 167..182
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 231
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LYG9"
FT CONFLICT 205
FT /note="T -> A (in Ref. 3; BAD94388)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 881 AA; 100366 MW; 42E3D5CBB471FBF1 CRC64;
MAAVDSTDRR DFIVNINGQE SGAVGATGSS SNAEGGNIWK ESSYDFWDGE KGKNDKKGDD
EDEDGGSFHF RQRGERRHSS AELSDPPSKL IGQFLHKQRA SGDEISLDVE LNMAELQSNT
PPRPATASNT PRRGLTTISE SSSPVKTKVK ADAVRRRQNR TSLGGSSDEE GRNRDEAEVL
KCGSKKPMLS RNKTKSRLQD PPTPTHPAID KTEMKSGRRS GIFKSGFLGK SPKAGTPGRN
GFEEEEEEDP FLDEDLPEEF KRDKLSFWVF LEWISLVLIV TSLVCSLTIH NLQRKTWWKL
DLWKWEVTVL VLICGRLVSS WIVRIIVFLV EKNFTWRKRV LYFVYGVRKS VQNCLWLGLV
LLAWHFLFDK KVERETRSTA LRYVTRVLVC LLVALIIWLV KTILVKVLAS SFHMSTYFDR
IQESLFTQYV IETLSGPPLM EIQRMEEEEQ QVAEDVKSLE KLAGAKLPPA LKATVKSFMK
VGKSPGLNRI GSKRGEDGEG IRIDQLKRMN TKNVSAWNMK RLMNIILKGA ISTLDQNMQD
TTQEDEDATH IRSEYEAKCA ARKIFHNVTE PGSRYIYLED FLRFLCEEEA ERAMALFEGA
SESDKISKSC LKNWVVKAFR ERRALALTLN DTKTAVDRLH RIINVVIGII IIIIWLLILG
IATTRFLLVL SSQLLLVAFV FGNSCKTIFE AIIFLFVMHP FDVGDRCEID GVQLVVEEMN
ILTTVFLRYD NQKIIYPNSV LGTKPIANYY RSPDMGDAVE FCVHIATPPE KITAIKQRIL
SYVDNKKDYW YPAPMIVFLS MDDLNSVKIA VWLTHRMNHQ DMGERYIRRG LLLEEVGKTC
RELDIEYRLY PLNINVRSLP PTANPTSSDR IPPSWMQQRG P
