MSL6_ARATH
ID MSL6_ARATH Reviewed; 856 AA.
AC Q9SYM1;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 122.
DE RecName: Full=Mechanosensitive ion channel protein 6;
DE AltName: Full=Mechanosensitive channel of small conductance-like 6;
DE AltName: Full=MscS-Like protein 6;
GN Name=MSL6; OrderedLocusNames=At1g78610; ORFNames=T30F21.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 728-856.
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP GENE FAMILY.
RX PubMed=12626684; DOI=10.1128/mmbr.67.1.66-85.2003;
RA Pivetti C.D., Yen M.R., Miller S., Busch W., Tseng Y.H., Booth I.R.,
RA Saier M.H. Jr.;
RT "Two families of mechanosensitive channel proteins.";
RL Microbiol. Mol. Biol. Rev. 67:66-85(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-462, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. La-0;
RX PubMed=14506206; DOI=10.1074/mcp.t300006-mcp200;
RA Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
RT "Large-scale analysis of in vivo phosphorylated membrane proteins by
RT immobilized metal ion affinity chromatography and mass spectrometry.";
RL Mol. Cell. Proteomics 2:1234-1243(2003).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=15308754; DOI=10.1105/tpc.104.023150;
RA Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
RT "Phosphoproteomics of the Arabidopsis plasma membrane and a new
RT phosphorylation site database.";
RL Plant Cell 16:2394-2405(2004).
RN [7]
RP REVIEW, GENE FAMILY, AND NOMENCLATURE.
RX DOI=10.1016/S1063-5823(06)58013-5;
RA Haswell E.S.;
RT "MscS-like proteins in plants.";
RL (In) Hamill O.P. (eds.);
RL Mechanosensitive Ion Channels, Part A, pp.329-349, Academic Press, San
RL Diego. (2007).
CC -!- FUNCTION: Mechanosensitive channel that opens in response to stretch
CC forces in the membrane lipid bilayer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family. {ECO:0000305}.
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DR EMBL; AC007260; AAD30575.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36127.1; -; Genomic_DNA.
DR EMBL; BX813849; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; G96814; G96814.
DR RefSeq; NP_177982.1; NM_106508.3.
DR AlphaFoldDB; Q9SYM1; -.
DR SMR; Q9SYM1; -.
DR BioGRID; 29416; 1.
DR STRING; 3702.AT1G78610.1; -.
DR iPTMnet; Q9SYM1; -.
DR PaxDb; Q9SYM1; -.
DR PRIDE; Q9SYM1; -.
DR ProteomicsDB; 239006; -.
DR EnsemblPlants; AT1G78610.1; AT1G78610.1; AT1G78610.
DR GeneID; 844197; -.
DR Gramene; AT1G78610.1; AT1G78610.1; AT1G78610.
DR KEGG; ath:AT1G78610; -.
DR Araport; AT1G78610; -.
DR TAIR; locus:2202965; AT1G78610.
DR eggNOG; KOG4629; Eukaryota.
DR HOGENOM; CLU_013552_0_0_1; -.
DR InParanoid; Q9SYM1; -.
DR OMA; FERNLSM; -.
DR OrthoDB; 609350at2759; -.
DR PhylomeDB; Q9SYM1; -.
DR PRO; PR:Q9SYM1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SYM1; baseline and differential.
DR Genevisible; Q9SYM1; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0008381; F:mechanosensitive ion channel activity; IBA:GO_Central.
DR GO; GO:0006820; P:anion transport; IBA:GO_Central.
DR Gene3D; 2.30.30.60; -; 1.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR016688; MscS-like_plants/fungi.
DR InterPro; IPR006685; MscS_channel.
DR InterPro; IPR023408; MscS_dom_sf.
DR PANTHER; PTHR31618; PTHR31618; 1.
DR Pfam; PF00924; MS_channel; 1.
DR PIRSF; PIRSF017209; Memb_At2g17000_prd; 1.
DR SUPFAM; SSF50182; SSF50182; 1.
PE 1: Evidence at protein level;
KW Ion channel; Ion transport; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..856
FT /note="Mechanosensitive ion channel protein 6"
FT /id="PRO_0000305186"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 323..343
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 360..380
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 615..635
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 651..671
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 45..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 116..226
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 66..84
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 129..144
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 145..179
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 211
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LYG9"
FT MOD_RES 462
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:14506206"
FT CONFLICT 850
FT /note="A -> T (in Ref. 3; BX813849)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 856 AA; 96604 MW; AE818CA8F76DBE2E CRC64;
MAVDAADRRE VIVKIDGENG NNNGVSGETV GKIWRDGSYD FWTDGEGNLN KGHNAAAVDS
DRSAATTGEQ QKDEGFEFRR GEDPPTKLIG QFLHKQQASG EICLDMDLGM DELQSRGLTP
VSESPRVSTK RDPVGRRDSR SNTNNNDDGE VVKCSGNNAP IQRSSSTLLK MRTRSRLSDP
PTPQLPPQTA DMKSGRIPKS GQMKSGFFGK SPKTQGEEEE DDPFAAEDLP EEYRKDKLSL
WIVLEWLSLI LIIAGFVCTL AIPSLRKKKL WELQLWKWES MVLVLICGRL VSSWIVKIVV
FFIERNFLLR KRVLYFVYGV RKAVQNCLWL GLVLLAWHFL FDEKVAKAAN TKALRVVTKI
FVCLLVGFLL WLVKTLLVKV LASSFHMSTY FDRIQESLFT QYVIETLSGP PLIEIQKNEE
EEERISVEVK KFQNPGGVEI QSGAQKSPMK TGKSPFLSHV LSNGGGGGGE NKGITIDSLH
KLNPKNVSAW KMKRLMNIIR NGSLTTLDEQ LQDPSLDDDK GNQIRSEFEA KLAARKIFHN
VAKPGSKFIY ANDIMRFLPD DEALKTLSLF EGASETNRIS KSSLKNWVVN AFRERRALAL
TLNDTKTAVN RLHKMVNIVV GIIILVIWLI ILGITSTKFL VVMSSQVVVV AFIFGNMCKI
VFESIIYLFV IHPFDVGDRC EIDGVQMVVE EMNILTTVFL RFDNQKVVYP NSLLWTKSIG
NYYRSPDMGD GIEFSIHITT PAEKIILIKQ RITSYIEGKK DHWYPAPMIV FKDMESLNSV
RIAVWPTHRM NHQDMGEKWA RRSQLVEEIA KICRELDIEY RLYPLDINVR NLPTSTALPV
SDRLPPNWSA PASGSN
