ID   MSL7_ARATH              Reviewed;         849 AA.
AC   F4IME1;
DT   25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=Mechanosensitive ion channel protein 7;
DE   AltName: Full=Mechanosensitive channel of small conductance-like 7;
DE   AltName: Full=MscS-Like protein 7;
GN   Name=MSL7; OrderedLocusNames=At2g17000; ORFNames=F6P23.16;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY.
RX   PubMed=12626684; DOI=10.1128/mmbr.67.1.66-85.2003;
RA   Pivetti C.D., Yen M.R., Miller S., Busch W., Tseng Y.H., Booth I.R.,
RA   Saier M.H. Jr.;
RT   "Two families of mechanosensitive channel proteins.";
RL   Microbiol. Mol. Biol. Rev. 67:66-85(2003).
RN   [4]
RP   REVIEW, GENE FAMILY, AND NOMENCLATURE.
RX   DOI=10.1016/S1063-5823(06)58013-5;
RA   Haswell E.S.;
RT   "MscS-like proteins in plants.";
RL   (In) Hamill O.P. (eds.);
RL   Mechanosensitive Ion Channels, Part A, pp.329-349, Academic Press, San
RL   Diego. (2007).
RN   [5]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=26973252; DOI=10.1016/j.jplph.2016.02.012;
RA   Nowak K., Gaj M.D.;
RT   "Stress-related function of bHLH109 in somatic embryo induction in
RT   Arabidopsis.";
RL   J. Plant Physiol. 193:119-126(2016).
CC   -!- FUNCTION: Mechanosensitive channel that opens in response to stretch
CC       forces in the membrane lipid bilayer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during somatic embryogenesis.
CC       {ECO:0000269|PubMed:26973252}.
CC   -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family. {ECO:0000305}.
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DR   EMBL; CP002685; AEC06571.1; -; Genomic_DNA.
DR   RefSeq; NP_179292.1; NM_127253.1.
DR   AlphaFoldDB; F4IME1; -.
DR   SMR; F4IME1; -.
DR   STRING; 3702.AT2G17000.1; -.
DR   PaxDb; F4IME1; -.
DR   PRIDE; F4IME1; -.
DR   EnsemblPlants; AT2G17000.1; AT2G17000.1; AT2G17000.
DR   GeneID; 816203; -.
DR   Gramene; AT2G17000.1; AT2G17000.1; AT2G17000.
DR   KEGG; ath:AT2G17000; -.
DR   Araport; AT2G17000; -.
DR   TAIR; locus:2827671; AT2G17000.
DR   eggNOG; KOG4629; Eukaryota.
DR   HOGENOM; CLU_013552_0_0_1; -.
DR   InParanoid; F4IME1; -.
DR   OrthoDB; 609350at2759; -.
DR   PRO; PR:F4IME1; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; F4IME1; baseline and differential.
DR   Genevisible; F4IME1; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0008381; F:mechanosensitive ion channel activity; IBA:GO_Central.
DR   GO; GO:0006820; P:anion transport; IBA:GO_Central.
DR   Gene3D; 2.30.30.60; -; 1.
DR   InterPro; IPR010920; LSM_dom_sf.
DR   InterPro; IPR016688; MscS-like_plants/fungi.
DR   InterPro; IPR006685; MscS_channel.
DR   InterPro; IPR023408; MscS_dom_sf.
DR   PANTHER; PTHR31618; PTHR31618; 2.
DR   Pfam; PF00924; MS_channel; 1.
DR   PIRSF; PIRSF017209; Memb_At2g17000_prd; 1.
DR   SUPFAM; SSF50182; SSF50182; 1.
PE   2: Evidence at transcript level;
KW   Ion channel; Ion transport; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..849
FT                   /note="Mechanosensitive ion channel protein 7"
FT                   /id="PRO_0000415329"
FT   TRANSMEM        231..251
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        274..294
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        313..333
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        344..364
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        606..626
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        642..662
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..49
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        21..37
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   849 AA;  97372 MW;  7E3949CB13577924 CRC64;
     MEFRKPFKSH SSYKQIISTG DQNEKTKKKK KLANLDDGDI AKTQSSGSSF DGNSYKFWQD
     IATDDYTKSG SFDFPQYREE ITLDVNEETE ETEDVSNNNN LSGSKETRVF FKINSSGTNN
     MSGSVRSCTS STSFSSATMR LNLEQQLEDE GEVVVRCSSV RKTELVSRAK ARSRLIDPPQ
     EEEQQYSSWI GTSDQLRSGL LGRHSDDIEE EDDSSAEEDV PVEYRKLKMD AITLLQWMSL
     IALVVALVLS LGLHTWRNAT LWSLHLWKWE VVLLVLICGR LVSGCGIRII VFFIERNFLL
     RKRVLYFVYG VKTAVQNCLW LGLVLLAWHF LFDKKVEKET QSDVLLLMSK ILVCFLLSTV
     LWLIKTLVVK VLASSFHVST YFDRIQEALF HHYLIETLSG PPMLELSRIE EEEDRTQDEI
     YKMQKGGADL SPELCSAAFP QEKSGSTMNM KFSPIIPKTG SDNGITMDDL HKMNQKNVSA
     WNMKRLMKIV RNVSLSTLDE QALQNTCEDE STRQIRSEKE AKAAARKIFK NVAQPGTKHI
     YLEDLMRFLR VDEAMKTMCL FEGALVTKKI TKSALKNWLV NAFRERRALA LTLNDTKTAV
     NKLHHMISFL TAIVIIVIWL ILLEIATSKY LLFLTSQVVL LAFMFGNSLK TVFESIIFLF
     IIHPYDVGDR LLIDTVEMVV EEMNILTTVF LRADNLKIVY PNILLWQKAI HNYNRSPDMG
     DEVTCCVHIT TPPEKIAAIK QRISSYIDSK PEYWYPKADV IVKDVEDLNI VRIAIWLCHK
     INHQNMGERF TRRALLIEEV IKILLELDIQ YRFHPLDINV KTMPTVVSSR VPPAWSQNPD
     LRRIILLEC