MSL8_ARATH
ID MSL8_ARATH Reviewed; 908 AA.
AC F4IME2;
DT 25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 2.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Mechanosensitive ion channel protein 8 {ECO:0000303|Ref.4};
DE AltName: Full=Mechanosensitive channel of small conductance-like 8 {ECO:0000303|Ref.4};
DE AltName: Full=MscS-Like protein 8 {ECO:0000303|Ref.4};
GN Name=MSL8 {ECO:0000303|Ref.4};
GN OrderedLocusNames=At2g17010 {ECO:0000312|Araport:AT2G17010};
GN ORFNames=F6P23.17 {ECO:0000312|EMBL:AEC06572.2};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=12626684; DOI=10.1128/mmbr.67.1.66-85.2003;
RA Pivetti C.D., Yen M.R., Miller S., Busch W., Tseng Y.H., Booth I.R.,
RA Saier M.H. Jr.;
RT "Two families of mechanosensitive channel proteins.";
RL Microbiol. Mol. Biol. Rev. 67:66-85(2003).
RN [4]
RP REVIEW, GENE FAMILY, AND NOMENCLATURE.
RX DOI=10.1016/S1063-5823(06)58013-5;
RA Haswell E.S.;
RT "MscS-like proteins in plants.";
RL (In) Hamill O.P. (eds.);
RL Mechanosensitive Ion Channels, Part A, pp.329-349, Academic Press, San
RL Diego. (2007).
RN [5]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ACTIVITY REGULATION, TISSUE
RP SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=26494758; DOI=10.1126/science.aac6014;
RA Hamilton E.S., Jensen G.S., Maksaev G., Katims A., Sherp A.M.,
RA Haswell E.S.;
RT "Mechanosensitive channel MSL8 regulates osmotic forces during pollen
RT hydration and germination.";
RL Science 350:438-441(2015).
CC -!- FUNCTION: Mechanosensitive channel that opens in response to stretch
CC forces in the membrane lipid bilayer. Exhibits a 6.3-fold preference
CC for chloride over sodium. Regulates osmotic forces during pollen
CC hydration and germination. {ECO:0000269|PubMed:26494758}.
CC -!- ACTIVITY REGULATION: Not regulated by MgCl(2), ruthenium red or
CC tetramethylammonium-Cl. {ECO:0000269|PubMed:26494758}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC Note=Has a unitary conductance of 57 pS under negative membrane
CC potentials and 39 pS under positive membrane potentials, and a gating
CC threshold pressure of -48.2 mm Hg. {ECO:0000269|PubMed:26494758};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:26494758};
CC Multi-pass membrane protein {ECO:0000255}. Endomembrane system
CC {ECO:0000269|PubMed:26494758}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in tricellular and mature pollen, and in
CC germinating tube. Not detected in leaves or roots.
CC {ECO:0000269|PubMed:26494758}.
CC -!- DISRUPTION PHENOTYPE: No visible morphological defects in the coat or
CC cell wall of desiccated pollen grains, but strongly decreased viability
CC during rehydration. {ECO:0000269|PubMed:26494758}.
CC -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family. {ECO:0000305}.
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DR EMBL; CP002685; AEC06572.2; -; Genomic_DNA.
DR RefSeq; NP_001318236.1; NM_001335527.1.
DR AlphaFoldDB; F4IME2; -.
DR SMR; F4IME2; -.
DR TCDB; 1.A.23.4.7; the small conductance mechanosensitive ion channel (mscs) family.
DR PaxDb; F4IME2; -.
DR PRIDE; F4IME2; -.
DR ProteomicsDB; 239008; -.
DR EnsemblPlants; AT2G17010.1; AT2G17010.1; AT2G17010.
DR GeneID; 816204; -.
DR Gramene; AT2G17010.1; AT2G17010.1; AT2G17010.
DR KEGG; ath:AT2G17010; -.
DR Araport; AT2G17010; -.
DR eggNOG; KOG4629; Eukaryota.
DR InParanoid; F4IME2; -.
DR OMA; AEVIRCT; -.
DR OrthoDB; 609350at2759; -.
DR PRO; PR:F4IME2; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4IME2; baseline and differential.
DR GO; GO:0012505; C:endomembrane system; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0008381; F:mechanosensitive ion channel activity; IBA:GO_Central.
DR GO; GO:0006820; P:anion transport; IBA:GO_Central.
DR Gene3D; 2.30.30.60; -; 1.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR016688; MscS-like_plants/fungi.
DR InterPro; IPR006685; MscS_channel.
DR InterPro; IPR023408; MscS_dom_sf.
DR PANTHER; PTHR31618; PTHR31618; 2.
DR Pfam; PF00924; MS_channel; 1.
DR PIRSF; PIRSF017209; Memb_At2g17000_prd; 1.
DR SUPFAM; SSF50182; SSF50182; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Ion channel; Ion transport; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..908
FT /note="Mechanosensitive ion channel protein 8"
FT /id="PRO_0000415330"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 341..361
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 381..401
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..431
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 673..693
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 709..729
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..88
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 148..172
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 190..221
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 242..265
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..22
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..66
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 72..86
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 154..172
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 192..221
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 242..257
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 908 AA; 104309 MW; 51CACE7EBEDD9530 CRC64;
MDFRNSFKSH SSYKQIRSPG DQSEPSPEHL PILHDHHPDH SGMVVDDQKP DSTRSSLDDG
RNAPVERDAS YKFWQDNTTG TSTDHTAVRT SDKDPIAISR KGDRLSGSFD FVHGKLPVDE
SPTKMVAGEP VNRQWRGRNN EEITLDVDQE NDDVSHQTMP TPTSTARTSF DASREMRVSF
NVRRAGGAFV AGSVPSSSSH SSSSSSATMR TNQDQPQLQE EEVVRCTSNM SFQRKSELIS
RVKTRSRLQD PPREEETPYS GWRSGQLKSG LLADIDEEDD PLAEEDVPDE YKRGKLDAIT
LLQWLSLVAI IAALACSLSI QSWKKVRVWN LHLWKWEVFL LVLICGRLVS GWGIRIVVFF
IERNFLLRKR VLYFVYGVRR AVQNCLWLGL VLLAWHFLFD KKVQRETRSR FLPYVTKILV
CFLLSTILWL IKTLVVKVLA SSFHVSTYFD RIQEALFNQY VIETLSGPPM IEMSRIEEEE
ERAQDEIFKM QNAGANLPPD LCAAAFPPGK SGRVMNPKLS PIIPKSTTDN GISMEHLHRM
NHKNISAWNM KRLMKIVRNV SLTTLDEQML ESTYEDESTR QIRSEKEAKA AARKIFKNVE
QRGAKYIYLE DLMRFLREDE AMKTMGLFEG APENKRISKS ALKNWLVNAF RERRALALTL
NDTKTAVNKL HHMINIVTAI VIVVIWLVLL EIASSKVLLF VSSQVVLLAF IFGNTVKTVF
ESIIFLFIVH PYDVGDRCEI DSVQLVVEEM NILTTVFLRY DNLKIMYPNS LLWQKSINNY
YRSPDMGDAI EFCVHITTPL EKISVIKQRI SNYIDNKPEY WYPQAKIIVK DLEDLHIVRL
AIWPCHRINH QDMAERWTRR AVLVEEVIKI LLELDIQHRF YPLDINVRTM PTVVSSRVPP
GWSQNQPA
