ID   MSMJL_METJA             Reviewed;         361 AA.
AC   Q58543;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 115.
DE   RecName: Full=Large-conductance mechanosensitive channel MscMJLR;
GN   OrderedLocusNames=MJ1143;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
RN   [2]
RP   FUNCTION AS A MECHANOSENSITIVE CHANNEL, AND SUBCELLULAR LOCATION.
RX   PubMed=11296222; DOI=10.1093/emboj/20.8.1888;
RA   Kloda A., Martinac B.;
RT   "Structural and functional differences between two homologous
RT   mechanosensitive channels of Methanococcus jannaschii.";
RL   EMBO J. 20:1888-1896(2001).
CC   -!- FUNCTION: Large-conductance mechanosensitive channel that opens in
CC       response to stretch forces in the membrane lipid bilayer. Selective for
CC       cations. Rectifies with voltage. {ECO:0000269|PubMed:11296222}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11296222};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:11296222}.
CC   -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family. {ECO:0000305}.
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DR   EMBL; L77117; AAB99143.1; -; Genomic_DNA.
DR   PIR; F64442; F64442.
DR   RefSeq; WP_010870654.1; NC_000909.1.
DR   AlphaFoldDB; Q58543; -.
DR   SMR; Q58543; -.
DR   STRING; 243232.MJ_1143; -.
DR   TCDB; 1.A.23.4.1; the small conductance mechanosensitive ion channel (mscs) family.
DR   EnsemblBacteria; AAB99143; AAB99143; MJ_1143.
DR   GeneID; 1452039; -.
DR   KEGG; mja:MJ_1143; -.
DR   eggNOG; arCOG01568; Archaea.
DR   HOGENOM; CLU_037945_0_1_2; -.
DR   InParanoid; Q58543; -.
DR   OMA; QNYKRLQ; -.
DR   OrthoDB; 58511at2157; -.
DR   PhylomeDB; Q58543; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   Gene3D; 2.30.30.60; -; 1.
DR   InterPro; IPR010920; LSM_dom_sf.
DR   InterPro; IPR006685; MscS_channel.
DR   InterPro; IPR011066; MscS_channel_C.
DR   InterPro; IPR006686; MscS_channel_CS.
DR   InterPro; IPR011014; MscS_channel_TM-2.
DR   InterPro; IPR023408; MscS_dom_sf.
DR   Pfam; PF00924; MS_channel; 1.
DR   SUPFAM; SSF50182; SSF50182; 1.
DR   SUPFAM; SSF82689; SSF82689; 1.
DR   SUPFAM; SSF82861; SSF82861; 1.
DR   PROSITE; PS01246; UPF0003; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Ion channel; Ion transport; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..361
FT                   /note="Large-conductance mechanosensitive channel MscMJLR"
FT                   /id="PRO_0000110254"
FT   TRANSMEM        20..40
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        65..85
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        89..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        137..157
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        177..197
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   361 AA;  40683 MW;  A6339C3C8AC4D68E CRC64;
     MTITQMISEI LMHNTVYNYI LSLISIILFI VIGKYANALI ERLADKLHKK SGIELDELLI
     RALSLPVAIA IILSGFYFGV NFLYLLPSLK TAVNEGILTA FILCVVVFFD RFLNELVERY
     LALTISKKTK KDVDDQIVVL TKKLVRLVVW VVGLLLILSN LGYDIKTLLA GLGIGGLAVA
     LASQNLVSNL IAGLIILTDK PFKIGNWITF SGGSGIVEDI GIRSTKIRAT DNSIIVVPNS
     KLIDEIIQNV PSKNKWKVST TIGVTYNTPV EKIRKAEEII KNILLEHPNV EDEPITVYFK
     EFGDWSLNIQ VVYYIKNSRY NGYQKYISTI NEVNLKIKEE FDRKGIEFAF PTYTLYLKRD
     D