ID   MSMJS_METJA             Reviewed;         350 AA.
AC   Q57634;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Small-conductance mechanosensitive channel MscMJ;
GN   OrderedLocusNames=MJ0170;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
RN   [2]
RP   FUNCTION AS A MECHANOSENSITIVE CHANNEL, AND SUBCELLULAR LOCATION.
RX   PubMed=11159397; DOI=10.1016/s0006-3495(01)76009-2;
RA   Kloda A., Martinac B.;
RT   "Molecular identification of a mechanosensitive channel in archaea.";
RL   Biophys. J. 80:229-240(2001).
CC   -!- FUNCTION: Small-conductance mechanosensitive channel that opens in
CC       response to stretch forces in the membrane lipid bilayer. Exhibits a
CC       sixfold preference for cations over anions. Non-rectifying.
CC       {ECO:0000269|PubMed:11159397}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11159397};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:11159397}.
CC   -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family. {ECO:0000305}.
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DR   EMBL; L77117; AAB98155.1; -; Genomic_DNA.
DR   PIR; C64321; C64321.
DR   AlphaFoldDB; Q57634; -.
DR   SMR; Q57634; -.
DR   STRING; 243232.MJ_0170; -.
DR   TCDB; 1.A.23.4.2; the small conductance mechanosensitive ion channel (mscs) family.
DR   EnsemblBacteria; AAB98155; AAB98155; MJ_0170.
DR   KEGG; mja:MJ_0170; -.
DR   eggNOG; arCOG01568; Archaea.
DR   HOGENOM; CLU_037945_0_1_2; -.
DR   InParanoid; Q57634; -.
DR   OMA; NIEFAFP; -.
DR   PhylomeDB; Q57634; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   Gene3D; 2.30.30.60; -; 1.
DR   InterPro; IPR010920; LSM_dom_sf.
DR   InterPro; IPR006685; MscS_channel.
DR   InterPro; IPR011066; MscS_channel_C.
DR   InterPro; IPR006686; MscS_channel_CS.
DR   InterPro; IPR011014; MscS_channel_TM-2.
DR   InterPro; IPR023408; MscS_dom_sf.
DR   Pfam; PF00924; MS_channel; 1.
DR   SUPFAM; SSF50182; SSF50182; 1.
DR   SUPFAM; SSF82689; SSF82689; 1.
DR   SUPFAM; SSF82861; SSF82861; 1.
DR   PROSITE; PS01246; UPF0003; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Ion channel; Ion transport; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..350
FT                   /note="Small-conductance mechanosensitive channel MscMJ"
FT                   /id="PRO_0000110253"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        59..79
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        130..150
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        154..174
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   350 AA;  39953 MW;  4EA0E5025086E7DF CRC64;
     MNMEIFGNSI SNILIFVVIT LLGIFIGKIV DKIVRNYLKK IIDKTKTKFD DIILESIDLP
     IIVLVVTLFF YFGLRFLILP DYILKLIDEA VKVVVILSAT YFAVKFIDGI FEHYLIPLTE
     KTETELDEHI IKPLKKVVKI LTILLGILTA LSSVGYDITA LLAGLGVGGL ALALAMQDTI
     KNFIAGILIL IDKPFSLGHW VKVKGAEGIV EEIGIRSTRI RTFDYTLITI PNSELLDSAI
     ENLTVRDRRR VLMTIGLTYN TPVEKIKRAK EIIKEIVENH PATLPPYRVH FREYGDWSLN
     LRVEYFVRNM GFDYYLNAVD EINLKIKEEF EKEGIEMAFP TYTVYLEKDN