MSMOA_DICDI
ID MSMOA_DICDI Reviewed; 267 AA.
AC Q55D52; C7FZW7; Q55D53;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 2.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=Putative methylsterol monooxygenase DDB_G0270946;
DE EC=1.14.18.9;
DE AltName: Full=C-4 methylsterol oxidase;
GN ORFNames=DDB_G0270946;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + 6 Fe(II)-
CC [cytochrome b5] + 5 H(+) + 3 O2 = 4alpha-carboxy-4beta-methyl-5alpha-
CC cholest-7-ene-3beta-ol + 6 Fe(III)-[cytochrome b5] + 4 H2O;
CC Xref=Rhea:RHEA:55220, Rhea:RHEA-COMP:10438, Rhea:RHEA-COMP:10439,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16455, ChEBI:CHEBI:29033, ChEBI:CHEBI:29034,
CC ChEBI:CHEBI:58387; EC=1.14.18.9;
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
CC -!- PATHWAY: Steroid biosynthesis; zymosterol biosynthesis; zymosterol from
CC lanosterol: step 3/6.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- DOMAIN: The histidine box domains may contain the active site and/or be
CC involved in metal ion binding.
CC -!- SIMILARITY: Belongs to the sterol desaturase family. {ECO:0000305}.
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DR EMBL; AAFI02000005; EEU04148.1; -; Genomic_DNA.
DR RefSeq; XP_002649198.1; XM_002649152.1.
DR AlphaFoldDB; Q55D52; -.
DR SMR; Q55D52; -.
DR STRING; 44689.DDB0304894; -.
DR PaxDb; Q55D52; -.
DR EnsemblProtists; EEU04148; EEU04148; DDB_G0270946.
DR GeneID; 8617234; -.
DR KEGG; ddi:DDB_G0270946; -.
DR dictyBase; DDB_G0270946; -.
DR eggNOG; KOG0873; Eukaryota.
DR HOGENOM; CLU_047036_5_3_1; -.
DR InParanoid; Q55D52; -.
DR OMA; EIMFYYA; -.
DR PhylomeDB; Q55D52; -.
DR Reactome; R-DDI-191273; Cholesterol biosynthesis.
DR Reactome; R-DDI-192105; Synthesis of bile acids and bile salts.
DR UniPathway; UPA00770; UER00756.
DR PRO; PR:Q55D52; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0000254; F:C-4 methylsterol oxidase activity; IBA:GO_Central.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0016126; P:sterol biosynthetic process; IBA:GO_Central.
DR InterPro; IPR006694; Fatty_acid_hydroxylase.
DR Pfam; PF04116; FA_hydroxylase; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; Iron; Lipid biosynthesis; Lipid metabolism;
KW Membrane; NAD; Oxidoreductase; Reference proteome; Steroid biosynthesis;
KW Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..267
FT /note="Putative methylsterol monooxygenase DDB_G0270946"
FT /id="PRO_0000380108"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 119..249
FT /note="Fatty acid hydroxylase"
FT /evidence="ECO:0000255"
FT MOTIF 132..136
FT /note="Histidine box-1"
FT /evidence="ECO:0000250"
FT MOTIF 145..149
FT /note="Histidine box-2"
FT /evidence="ECO:0000250"
FT MOTIF 224..230
FT /note="Histidine box-3"
FT /evidence="ECO:0000250"
SQ SEQUENCE 267 AA; 31445 MW; 3B8B984165A47A8D CRC64;
MESLSLKFIE PYWFKFVDYY GEEFLLTYGT FIAHQVFYFG CFIPFLIADF IPFFRKYKIQ
QTKENDWKSQ TYCAIKVILT QVLIQLPMMY IFDPAIKAIG LSARAPLPSI PYLLLTLVSS
FIIEDFYFYW AHRALHHGIW YKYIHKVHHD YASPFGITAE YAHPLETIIL GVGTVIGPFL
FSRDLFTLWV WLGVRLYQTV ECHSGYDFPW SFTNLIPFWG GAPFHDYHHE VFIGNYASTF
TYLDKIFGTS GKSYYSRIEK KSINKSE
