MSN4_YEAST
ID MSN4_YEAST Reviewed; 630 AA.
AC P33749; D6VXM5; P35726;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 195.
DE RecName: Full=Zinc finger protein MSN4;
DE AltName: Full=Multicopy suppressor of SNF1 protein 4;
GN Name=MSN4; OrderedLocusNames=YKL062W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8321194; DOI=10.1128/mcb.13.7.3872-3881.1993;
RA Estruch F., Carlson M.;
RT "Two homologous zinc finger genes identified by multicopy suppression in a
RT SNF1 protein kinase mutant of Saccharomyces cerevisiae.";
RL Mol. Cell. Biol. 13:3872-3881(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091862; DOI=10.1002/yea.320100008;
RA Rasmussen S.W.;
RT "Sequence of a 28.6 kb region of yeast chromosome XI includes the FBA1 and
RT TOA2 genes, an open reading frame (ORF) similar to a translationally
RT controlled tumour protein, one ORF containing motifs also found in plant
RT storage proteins and 13 ORFs with weak or no homology to known proteins.";
RL Yeast 10:S63-S68(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP CHARACTERIZATION.
RX PubMed=8641288; DOI=10.1002/j.1460-2075.1996.tb00576.x;
RA Martinez-Pastor M.T., Marchler G., Schueller C., Marchler-Bauer A.,
RA Ruis H., Estruch F.;
RT "The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are
RT required for transcriptional induction through the stress response element
RT (STRE).";
RL EMBO J. 15:2227-2235(1996).
RN [6]
RP NUCLEOCYTOPLASMIC SHUTTLING.
RX PubMed=12732613; DOI=10.1083/jcb.200303030;
RA Jacquet M., Renault G., Lallet S., De Mey J., Goldbeter A.;
RT "Oscillatory nucleocytoplasmic shuttling of the general stress response
RT transcriptional activators Msn2 and Msn4 in Saccharomyces cerevisiae.";
RL J. Cell Biol. 161:497-505(2003).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-558, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=YAL6B;
RX PubMed=15665377; DOI=10.1074/mcp.m400219-mcp200;
RA Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M.,
RA Jensen O.N.;
RT "Quantitative phosphoproteomics applied to the yeast pheromone signaling
RT pathway.";
RL Mol. Cell. Proteomics 4:310-327(2005).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-263; SER-319 AND SER-558, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-558, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT "Analysis of phosphorylation sites on proteins from Saccharomyces
RT cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-479, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [11]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-178; SER-263; SER-316 AND
RP SER-558, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [12]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [13]
RP DOMAIN.
RX PubMed=27618436; DOI=10.1371/journal.pone.0162842;
RA Piskacek M., Havelka M., Rezacova M., Knight A.;
RT "The 9aaTAD transactivation domains: From Gal4 to p53.";
RL PLoS ONE 11:E0162842-E0162842(2016).
CC -!- FUNCTION: Positive transcriptional factor that acts as a component of
CC the stress responsive system. Recognizes and binds to the stress
CC response element (STRE) which is involved in the response to various
CC forms of stress (heat, oxidative, osmotic, etc.). Involved in the
CC regulation of the CTT1, DDR2, HSP12 genes.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
CC -!- DOMAIN: the 9aaTAD motif (residues 237 to 245) is a transactivation
CC domain present in a large number of yeast and animal transcription
CC factors. {ECO:0000305|PubMed:27618436}.
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DR EMBL; L08839; AAA34807.1; -; Genomic_DNA.
DR EMBL; X75781; CAA53421.1; -; Genomic_DNA.
DR EMBL; Z28062; CAA81899.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09095.1; -; Genomic_DNA.
DR PIR; S37884; S37884.
DR RefSeq; NP_012861.1; NM_001179628.1.
DR AlphaFoldDB; P33749; -.
DR SMR; P33749; -.
DR BioGRID; 34071; 158.
DR DIP; DIP-6574N; -.
DR IntAct; P33749; 59.
DR MINT; P33749; -.
DR STRING; 4932.YKL062W; -.
DR iPTMnet; P33749; -.
DR MaxQB; P33749; -.
DR PaxDb; P33749; -.
DR PRIDE; P33749; -.
DR EnsemblFungi; YKL062W_mRNA; YKL062W; YKL062W.
DR GeneID; 853803; -.
DR KEGG; sce:YKL062W; -.
DR SGD; S000001545; MSN4.
DR VEuPathDB; FungiDB:YKL062W; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000174779; -.
DR HOGENOM; CLU_024342_0_0_1; -.
DR InParanoid; P33749; -.
DR OMA; ISHSIEF; -.
DR BioCyc; YEAST:G3O-31860-MON; -.
DR PRO; PR:P33749; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P33749; protein.
DR GO; GO:0005829; C:cytosol; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IDA:SGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:SGD.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006338; P:chromatin remodeling; IGI:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:0043619; P:regulation of transcription from RNA polymerase II promoter in response to oxidative stress; IBA:GO_Central.
DR GO; GO:0042594; P:response to starvation; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 2.
DR SMART; SM00355; ZnF_C2H2; 2.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 2.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE 1: Evidence at protein level;
KW Acetylation; Activator; Cytoplasm; DNA-binding; Metal-binding; Nucleus;
KW Phosphoprotein; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..630
FT /note="Zinc finger protein MSN4"
FT /id="PRO_0000046811"
FT ZN_FING 573..596
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 602..624
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 37..77
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 115..137
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 292..322
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 360..379
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 502..566
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 237..245
FT /note="9aaTAD"
FT /evidence="ECO:0000269|PubMed:27618436"
FT COMPBIAS 502..551
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT MOD_RES 178
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 263
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:19779198"
FT MOD_RES 316
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 319
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 479
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 558
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:15665377,
FT ECO:0007744|PubMed:17287358, ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:19779198"
SQ SEQUENCE 630 AA; 69723 MW; 3A264FB37C82C385 CRC64;
MLVFGPNSSF VRHANKKQED SSIMNEPNGL MDPVLSTTNV SATSSNDNSA NNSISSPEYT
FGQFSMDSPH RTDATNTPIL TATTNTTANN SLMNLKDTAS LATNWKWKNS NNAQFVNDGE
KQSSNANGKK NGGDKIYSSV ATPQALNDEL KNLEQLEKVF SPMNPINDSH FNENIELSPH
QHATSPKTNL LEAEPSIYSN LFLDARLPNN ANSTTGLNDN DYNLDDTNND NTNSMQSILE
DFVSSEEALK FMPDAGRDAR RYSEVVTSSF PSMTDSRNSI SHSIEFWNLN HKNSSNSKPT
QQIIPEGTAT TERRGSTISP TTTINNSNPN FKLLDHDVSQ ALSGYSMDFS KDSGITKPKS
ISSSLNRISH SSSTTRQQRA SLPLIHDIES FANDSVMANP LSDSASFLSE ENEDDAFGAL
NYNSLDATTM SAFDNNVDPF NILKSSPAQD QQFIKPSMML SDNASAAAKL ATSGVDNITP
TPAFQRRSYD ISMNSSFKIL PTSQAHHAAQ HHQQQPTKQA TVSPNTRRRK SSSVTLSPTI
SHNNNNGKVP VQPRKRKSIT TIDPNNYDKN KPFKCKDCEK AFRRSEHLKR HIRSVHSTER
PFACMFCEKK FSRSDNLSQH LKTHKKHGDF
