ID   MSOX_STRAW              Reviewed;         384 AA.
AC   Q827H4;
DT   04-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Monomeric sarcosine oxidase {ECO:0000255|HAMAP-Rule:MF_00516};
DE            Short=MSOX {ECO:0000255|HAMAP-Rule:MF_00516};
DE            EC=1.5.3.1 {ECO:0000255|HAMAP-Rule:MF_00516};
GN   Name=soxA {ECO:0000255|HAMAP-Rule:MF_00516}; Synonyms=solA;
GN   OrderedLocusNames=SAV_6950;
OS   Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC
OS   14893 / NCIMB 12804 / NRRL 8165 / MA-4680).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=227882;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL
RC   8165 / MA-4680;
RX   PubMed=11572948; DOI=10.1073/pnas.211433198;
RA   Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M.,
RA   Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T.,
RA   Sakaki Y., Hattori M.;
RT   "Genome sequence of an industrial microorganism Streptomyces avermitilis:
RT   deducing the ability of producing secondary metabolites.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL
RC   8165 / MA-4680;
RX   PubMed=12692562; DOI=10.1038/nbt820;
RA   Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T.,
RA   Sakaki Y., Hattori M., Omura S.;
RT   "Complete genome sequence and comparative analysis of the industrial
RT   microorganism Streptomyces avermitilis.";
RL   Nat. Biotechnol. 21:526-531(2003).
CC   -!- FUNCTION: Catalyzes the oxidative demethylation of sarcosine.
CC       {ECO:0000255|HAMAP-Rule:MF_00516}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O2 + sarcosine = formaldehyde + glycine + H2O2;
CC         Xref=Rhea:RHEA:13313, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16240, ChEBI:CHEBI:16842, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:57433; EC=1.5.3.1; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00516};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00516};
CC       Note=Binds 1 FAD per subunit. {ECO:0000255|HAMAP-Rule:MF_00516};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00516}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00516}.
CC   -!- SIMILARITY: Belongs to the MSOX/MTOX family. MSOX subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00516}.
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DR   EMBL; BA000030; BAC74661.1; -; Genomic_DNA.
DR   RefSeq; WP_010988347.1; NZ_JZJK01000082.1.
DR   AlphaFoldDB; Q827H4; -.
DR   SMR; Q827H4; -.
DR   STRING; 227882.SAV_6950; -.
DR   EnsemblBacteria; BAC74661; BAC74661; SAVERM_6950.
DR   KEGG; sma:SAVERM_6950; -.
DR   eggNOG; COG0665; Bacteria.
DR   HOGENOM; CLU_007884_2_1_11; -.
DR   OMA; EMYSDPV; -.
DR   OrthoDB; 912110at2; -.
DR   Proteomes; UP000000428; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0008115; F:sarcosine oxidase activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.50.50.60; -; 1.
DR   HAMAP; MF_00516; MSOX; 1.
DR   InterPro; IPR002204; 3-OH-isobutyrate_DH-rel_CS.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR045170; MTOX.
DR   InterPro; IPR006281; SoxA_mon.
DR   PANTHER; PTHR10961; PTHR10961; 1.
DR   Pfam; PF01266; DAO; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; FAD; Flavoprotein; Oxidoreductase; Reference proteome.
FT   CHAIN           1..384
FT                   /note="Monomeric sarcosine oxidase"
FT                   /id="PRO_0000213763"
FT   BINDING         6..36
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00516"
FT   MOD_RES         315
FT                   /note="S-8alpha-FAD cysteine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00516"
SQ   SEQUENCE   384 AA;  42167 MW;  28302788BB0DE585 CRC64;
     MSPTYDVIVI GLGGMGSAAA HHLSARGARV LGLEKFGPVH NRGSSHGGSR ITRQSYFEDP
     AYVPLLLRSY ELYEEVERST GREVATLSGG VMVGRPDSLT VAGSLRSATQ WDLPHEMLDA
     KEIRRRFPTL NPSNDEVALY EKKAGLVRPE NMVAAHLQLA TRQGAELHFE EPMTRWEPYR
     DGVRVHTAEN TYTAGQLVIC PGAWAPQLLT DLGVPFTIER QVMYWFQPRH GVGPFRPENH
     PIYIWEDAEG VQVYGFPSID GPDLGAKVAF FRKGVVCTPE TIDRTVHDHE VQAMADHMSR
     CIPDLPGTFL KAATCMYSNT PDEHFVIARH PAHPDSVTVA CGFSGHGFKF VPVVGEIVAD
     LALTGTTAHP IGLFDPRRLA AAPA