MSPD1_MOUSE
ID MSPD1_MOUSE Reviewed; 213 AA.
AC Q8VEL0; Q497P5; Q9D8Y9;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Motile sperm domain-containing protein 1;
GN Name=Mospd1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Pancreas;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor, Molar, and Olfactory epithelium;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, Kidney, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP INDUCTION, MOTIF, AND MUTAGENESIS OF 205-GLY--THR-213.
RX PubMed=21792907; DOI=10.1002/jcp.22595;
RA Thaler R., Rumpler M., Spitzer S., Klaushofer K., Varga F.;
RT "Mospd1, a new player in mesenchymal versus epidermal cell
RT differentiation.";
RL J. Cell. Physiol. 226:2505-2515(2011).
RN [5]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=26175344; DOI=10.1002/stem.2102;
RA Kara M., Axton R.A., Jackson M., Ghaffari S., Buerger K., Watt A.J.,
RA Taylor A.H., Orr B., Hardy W.R., Peault B., Forrester L.M.;
RT "A role for MOSPD1 in mesenchymal stem cell proliferation and
RT differentiation.";
RL Stem Cells 33:3077-3086(2015).
CC -!- FUNCTION: Plays a role in differentiation and/or proliferation of
CC mesenchymal stem cells (PubMed:21792907, PubMed:26175344). Proposed to
CC be involved in epithelial-to-mesenchymal transition (EMT)
CC (PubMed:21792907). However, another study suggests that it is not
CC required for EMT or stem cell self-renewal and acts during later stages
CC of differentiation (PubMed:26175344). {ECO:0000269|PubMed:21792907,
CC ECO:0000269|PubMed:26175344}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000305|PubMed:21792907}; Multi-pass membrane protein
CC {ECO:0000255}. Golgi apparatus membrane {ECO:0000305|PubMed:21792907};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8VEL0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VEL0-2; Sequence=VSP_014045;
CC -!- TISSUE SPECIFICITY: Widely expressed. Shows highest expression in ribs,
CC and slightly lower levels of expression in heart, kidney, muscle,
CC thymus, calvariae and lung. Also detected at low levels in spleen and
CC liver. {ECO:0000269|PubMed:21792907}.
CC -!- DEVELOPMENTAL STAGE: Expressed at low levels in undifferentiated
CC mesenchymal stem cells and then shows increasing expression levels as
CC differentiation proceeds. {ECO:0000269|PubMed:21792907,
CC ECO:0000269|PubMed:26175344}.
CC -!- INDUCTION: May be up-regulated in response to cell-cell contact.
CC {ECO:0000269|PubMed:21792907}.
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DR EMBL; AK007533; BAB25094.1; -; mRNA.
DR EMBL; BC018329; AAH18329.1; -; mRNA.
DR EMBL; BC085100; AAH85100.1; -; mRNA.
DR EMBL; BC100442; AAI00443.1; -; mRNA.
DR CCDS; CCDS30131.1; -. [Q8VEL0-2]
DR CCDS; CCDS72381.1; -. [Q8VEL0-1]
DR RefSeq; NP_001277443.1; NM_001290514.1. [Q8VEL0-1]
DR RefSeq; NP_081685.1; NM_027409.5. [Q8VEL0-2]
DR RefSeq; XP_006541603.1; XM_006541540.3. [Q8VEL0-1]
DR RefSeq; XP_017174106.1; XM_017318617.1. [Q8VEL0-1]
DR AlphaFoldDB; Q8VEL0; -.
DR SMR; Q8VEL0; -.
DR STRING; 10090.ENSMUSP00000023836; -.
DR PhosphoSitePlus; Q8VEL0; -.
DR MaxQB; Q8VEL0; -.
DR PaxDb; Q8VEL0; -.
DR PRIDE; Q8VEL0; -.
DR ProteomicsDB; 291421; -. [Q8VEL0-1]
DR ProteomicsDB; 291422; -. [Q8VEL0-2]
DR Antibodypedia; 30323; 50 antibodies from 18 providers.
DR DNASU; 70380; -.
DR Ensembl; ENSMUST00000023836; ENSMUSP00000023836; ENSMUSG00000023074. [Q8VEL0-2]
DR Ensembl; ENSMUST00000096447; ENSMUSP00000094182; ENSMUSG00000023074. [Q8VEL0-1]
DR Ensembl; ENSMUST00000152743; ENSMUSP00000129567; ENSMUSG00000023074. [Q8VEL0-1]
DR GeneID; 70380; -.
DR KEGG; mmu:70380; -.
DR UCSC; uc009tfa.2; mouse. [Q8VEL0-2]
DR UCSC; uc009tfb.2; mouse. [Q8VEL0-1]
DR CTD; 56180; -.
DR MGI; MGI:1917630; Mospd1.
DR VEuPathDB; HostDB:ENSMUSG00000023074; -.
DR eggNOG; KOG0439; Eukaryota.
DR GeneTree; ENSGT00940000155266; -.
DR HOGENOM; CLU_088040_0_0_1; -.
DR InParanoid; Q8VEL0; -.
DR OMA; KVLCNAP; -.
DR OrthoDB; 1197131at2759; -.
DR PhylomeDB; Q8VEL0; -.
DR TreeFam; TF319778; -.
DR BioGRID-ORCS; 70380; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Mospd1; mouse.
DR PRO; PR:Q8VEL0; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q8VEL0; protein.
DR Bgee; ENSMUSG00000023074; Expressed in animal zygote and 259 other tissues.
DR Genevisible; Q8VEL0; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:BHF-UCL.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IDA:BHF-UCL.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:BHF-UCL.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:BHF-UCL.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR039283; MOSPD1.
DR InterPro; IPR000535; MSP_dom.
DR InterPro; IPR008962; PapD-like_sf.
DR PANTHER; PTHR34441; PTHR34441; 1.
DR Pfam; PF00635; Motile_Sperm; 1.
DR SUPFAM; SSF49354; SSF49354; 1.
DR PROSITE; PS50202; MSP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Differentiation; Endoplasmic reticulum;
KW Golgi apparatus; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..213
FT /note="Motile sperm domain-containing protein 1"
FT /id="PRO_0000213460"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 16..143
FT /note="MSP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00132"
FT MOTIF 205..208
FT /note="Nuclear export signal"
FT /evidence="ECO:0000269|PubMed:21792907"
FT VAR_SEQ 149..150
FT /note="PE -> PGK (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_014045"
FT MUTAGEN 204..213
FT /note="Missing: Localizes to the nucleus instead of the
FT cytoplasm."
FT /evidence="ECO:0000269|PubMed:21792907"
SQ SEQUENCE 213 AA; 24074 MW; FB72756A0528CB34 CRC64;
MHQQKRQPEL VEGNLPVFVF PTELIFYADD QSTHKQVLTL YNPYEFALKF KVLCTTPNKY
VVVDAAGAVK PQCCVDIVIR HRDVRSCHYG VIDKFRLQVS EQSQRKALGR KEVIATLLPS
AKEQQKEEEE KRIKEHLTES VFFEQSCQPE NRAVSSGPSL LTVFLGVVCI AALMLPTLGD
MESLVPLYLH LSVNQKLVAA YILGLITMAI LRT
