MSPD3_HUMAN
ID MSPD3_HUMAN Reviewed; 235 AA.
AC O75425; A4D2D1; A6NG17; C9JE89; D6W5W1; O75423; O75424;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Motile sperm domain-containing protein 3;
GN Name=MOSPD3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1; 2 AND 3).
RX PubMed=9799793; DOI=10.1101/gr.8.10.1060;
RA Gloeckner G., Scherer S., Schattevoy R., Boright A.P., Weber J.,
RA Tsui L.-C., Rosenthal A.;
RT "Large-scale sequencing of two regions in human chromosome 7q22: analysis
RT of 650 kb of genomic sequence around the EPO and CUTL1 loci reveals 17
RT genes.";
RL Genome Res. 8:1060-1073(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12690205; DOI=10.1126/science.1083423;
RA Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA Adams M.D., Tsui L.-C.;
RT "Human chromosome 7: DNA sequence and biology.";
RL Science 300:767-772(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
RC TISSUE=B-cell, Eye, and Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- INTERACTION:
CC O75425; Q3SXY8: ARL13B; NbExp=3; IntAct=EBI-12179105, EBI-11343438;
CC O75425; P18859: ATP5PF; NbExp=3; IntAct=EBI-12179105, EBI-2606700;
CC O75425; P11912: CD79A; NbExp=3; IntAct=EBI-12179105, EBI-7797864;
CC O75425; Q969F0: FATE1; NbExp=3; IntAct=EBI-12179105, EBI-743099;
CC O75425; Q8TBE3: FNDC9; NbExp=3; IntAct=EBI-12179105, EBI-12142257;
CC O75425; Q8TDT2: GPR152; NbExp=3; IntAct=EBI-12179105, EBI-13345167;
CC O75425; Q7Z5P4: HSD17B13; NbExp=3; IntAct=EBI-12179105, EBI-18053395;
CC O75425; Q9Y5U9: IER3IP1; NbExp=3; IntAct=EBI-12179105, EBI-725665;
CC O75425; Q8N6L0: KASH5; NbExp=3; IntAct=EBI-12179105, EBI-749265;
CC O75425; Q9GZY8-5: MFF; NbExp=3; IntAct=EBI-12179105, EBI-11956541;
CC O75425; Q7Z6M3: MILR1; NbExp=3; IntAct=EBI-12179105, EBI-18391669;
CC O75425; Q9H6H4: REEP4; NbExp=3; IntAct=EBI-12179105, EBI-7545592;
CC O75425; Q86VR2: RETREG3; NbExp=3; IntAct=EBI-12179105, EBI-10192441;
CC O75425; Q5EBL4-3: RILPL1; NbExp=3; IntAct=EBI-12179105, EBI-12072024;
CC O75425; Q9NR31: SAR1A; NbExp=3; IntAct=EBI-12179105, EBI-3920694;
CC O75425; O15393-2: TMPRSS2; NbExp=3; IntAct=EBI-12179105, EBI-12345267;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=O75425-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O75425-2; Sequence=VSP_014053;
CC Name=3;
CC IsoId=O75425-3; Sequence=VSP_014054, VSP_014055;
CC Name=4;
CC IsoId=O75425-4; Sequence=VSP_044522;
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DR EMBL; AF053356; AAC78797.1; -; Genomic_DNA.
DR EMBL; AF053356; AAC78798.1; -; Genomic_DNA.
DR EMBL; AF053356; AAC78799.1; -; Genomic_DNA.
DR EMBL; AC099394; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH236956; EAL23824.1; -; Genomic_DNA.
DR EMBL; CH471091; EAW76511.1; -; Genomic_DNA.
DR EMBL; CH471091; EAW76512.1; -; Genomic_DNA.
DR EMBL; CH471091; EAW76513.1; -; Genomic_DNA.
DR EMBL; BC005042; AAH05042.1; -; mRNA.
DR EMBL; BC011653; AAH11653.1; -; mRNA.
DR EMBL; BC028475; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS47662.1; -. [O75425-4]
DR CCDS; CCDS5701.1; -. [O75425-1]
DR RefSeq; NP_001035186.1; NM_001040097.1. [O75425-1]
DR RefSeq; NP_001035187.1; NM_001040098.1. [O75425-1]
DR RefSeq; NP_001035188.1; NM_001040099.1. [O75425-4]
DR RefSeq; NP_076438.1; NM_023948.4. [O75425-1]
DR RefSeq; XP_005250586.1; XM_005250529.4.
DR RefSeq; XP_005250588.1; XM_005250531.3.
DR AlphaFoldDB; O75425; -.
DR SMR; O75425; -.
DR BioGRID; 122217; 25.
DR IntAct; O75425; 16.
DR STRING; 9606.ENSP00000377522; -.
DR iPTMnet; O75425; -.
DR PhosphoSitePlus; O75425; -.
DR BioMuta; MOSPD3; -.
DR jPOST; O75425; -.
DR MassIVE; O75425; -.
DR MaxQB; O75425; -.
DR PaxDb; O75425; -.
DR PeptideAtlas; O75425; -.
DR PRIDE; O75425; -.
DR ProteomicsDB; 49993; -. [O75425-1]
DR ProteomicsDB; 49994; -. [O75425-2]
DR ProteomicsDB; 49995; -. [O75425-3]
DR ProteomicsDB; 9827; -.
DR Antibodypedia; 30772; 123 antibodies from 24 providers.
DR DNASU; 64598; -.
DR Ensembl; ENST00000223054.8; ENSP00000223054.4; ENSG00000106330.12. [O75425-1]
DR Ensembl; ENST00000379527.6; ENSP00000368842.2; ENSG00000106330.12. [O75425-1]
DR Ensembl; ENST00000393950.7; ENSP00000377522.2; ENSG00000106330.12. [O75425-1]
DR Ensembl; ENST00000424091.2; ENSP00000404626.2; ENSG00000106330.12. [O75425-4]
DR GeneID; 64598; -.
DR KEGG; hsa:64598; -.
DR MANE-Select; ENST00000393950.7; ENSP00000377522.2; NM_023948.5; NP_076438.1.
DR UCSC; uc003uvq.4; human. [O75425-1]
DR CTD; 64598; -.
DR DisGeNET; 64598; -.
DR GeneCards; MOSPD3; -.
DR HGNC; HGNC:25078; MOSPD3.
DR HPA; ENSG00000106330; Low tissue specificity.
DR MIM; 609125; gene.
DR neXtProt; NX_O75425; -.
DR OpenTargets; ENSG00000106330; -.
DR PharmGKB; PA134865874; -.
DR VEuPathDB; HostDB:ENSG00000106330; -.
DR eggNOG; KOG0439; Eukaryota.
DR GeneTree; ENSGT00940000162123; -.
DR HOGENOM; CLU_088040_0_0_1; -.
DR InParanoid; O75425; -.
DR OMA; FRIELSQ; -.
DR OrthoDB; 1197131at2759; -.
DR PhylomeDB; O75425; -.
DR TreeFam; TF319778; -.
DR PathwayCommons; O75425; -.
DR SignaLink; O75425; -.
DR BioGRID-ORCS; 64598; 13 hits in 1073 CRISPR screens.
DR ChiTaRS; MOSPD3; human.
DR GenomeRNAi; 64598; -.
DR Pharos; O75425; Tbio.
DR PRO; PR:O75425; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; O75425; protein.
DR Bgee; ENSG00000106330; Expressed in right testis and 197 other tissues.
DR ExpressionAtlas; O75425; baseline and differential.
DR Genevisible; O75425; HS.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0033149; F:FFAT motif binding; IBA:GO_Central.
DR GO; GO:0090158; P:endoplasmic reticulum membrane organization; IBA:GO_Central.
DR GO; GO:0061817; P:endoplasmic reticulum-plasma membrane tethering; IBA:GO_Central.
DR GO; GO:0007507; P:heart development; IEA:Ensembl.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR000535; MSP_dom.
DR InterPro; IPR008962; PapD-like_sf.
DR InterPro; IPR016763; VAP.
DR PANTHER; PTHR10809; PTHR10809; 1.
DR Pfam; PF00635; Motile_Sperm; 1.
DR SUPFAM; SSF49354; SSF49354; 1.
DR PROSITE; PS50202; MSP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..235
FT /note="Motile sperm domain-containing protein 3"
FT /id="PRO_0000213465"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 33..145
FT /note="MSP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00132"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 143..171
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 145..163
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..53
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_014054"
FT VAR_SEQ 1..23
FT /note="MRRGAPQDQELVGPGPPGRGSRG -> MSDRPEPAC (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_014053"
FT VAR_SEQ 54..68
FT /note="LLTLYNPTGTALRFR -> MTHMAGLVCVCLYPP (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_014055"
FT VAR_SEQ 95..104
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_044522"
SQ SEQUENCE 235 AA; 25519 MW; 405D69B8309C4FAF CRC64;
MRRGAPQDQE LVGPGPPGRG SRGAPPPLGP VVPVLVFPPD LVFRADQRSG PRQLLTLYNP
TGTALRFRVL CTAPAKYTVF DAEGYVKPQS CIDIVIRHVA PIPSHYDVQD RFRIELSEEG
AEGRVVGRKD ITSILRAPAY PLELQGQPDP APRPGPPAGT PPPTARHFQE HPRQQLATSS
FLLFLLTGIV SVAFLLLPLP DELGSQLPQV LHVSLGQKLV AAYVLGLLTM VFLRT
