ID   MSPI2_MELSA             Reviewed;          36 AA.
AC   B3A0N9;
DT   14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   25-MAY-2022, entry version 21.
DE   RecName: Full=Serine protease inhibitor 2;
DE   AltName: Full=Protease inhibitor MSPI-2;
OS   Melanoplus sanguinipes (Migratory grasshopper).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Polyneoptera; Orthoptera; Caelifera; Acrididea; Acridomorpha;
OC   Acridoidea; Acrididae; Melanoplinae; Melanoplini; Melanoplus.
OX   NCBI_TaxID=65742;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   TISSUE=Hemolymph {ECO:0000269|Ref.1};
RA   Taub-Montemayor T.E., Jones N.T., Linse K.D., Rankin M.A.;
RT   "Pacifastin-related serine protease inhibitors of the migratory
RT   grasshopper, Melanoplus sanguinipes.";
RL   Submitted (OCT-2011) to UniProtKB.
CC   -!- FUNCTION: Probable serine protease inhibitor.
CC       {ECO:0000250|UniProtKB:O46162}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}.
CC   -!- TISSUE SPECIFICITY: Expressed in hemolymph. {ECO:0000269|Ref.1}.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I19 family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00776}.
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DR   AlphaFoldDB; B3A0N9; -.
DR   SMR; B3A0N9; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   InterPro; IPR008037; Pacifastin_dom.
DR   InterPro; IPR036201; Pacifastin_dom_sf.
DR   InterPro; IPR016307; Prtase-inh_pacifastin.
DR   Pfam; PF05375; Pacifastin_I; 1.
DR   PIRSF; PIRSF001625; Prot_inhib_pacifastin; 1.
DR   SUPFAM; SSF57283; SSF57283; 1.
DR   PROSITE; PS51446; PACIFASTIN; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor; Secreted;
KW   Serine protease inhibitor.
FT   PEPTIDE         1..36
FT                   /note="Serine protease inhibitor 2"
FT                   /evidence="ECO:0000269|Ref.1"
FT                   /id="PRO_0000414707"
FT   DOMAIN          1..36
FT                   /note="Pacifastin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00776"
FT   SITE            31..32
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250|UniProtKB:O46162,
FT                   ECO:0000255|PROSITE-ProRule:PRU00776"
FT   DISULFID        4..19
FT                   /evidence="ECO:0000250|UniProtKB:O46162,
FT                   ECO:0000255|PROSITE-ProRule:PRU00776"
FT   DISULFID        14..34
FT                   /evidence="ECO:0000250|UniProtKB:O46162,
FT                   ECO:0000255|PROSITE-ProRule:PRU00776"
FT   DISULFID        17..29
FT                   /evidence="ECO:0000250|UniProtKB:O46162,
FT                   ECO:0000255|PROSITE-ProRule:PRU00776"
SQ   SEQUENCE   36 AA;  3752 MW;  AECAFE1329D3D3BA CRC64;
     EISCEPGTTF QDKCNTCRCG KDGKSAAGCT LKACPQ