MSRA2_STAA8
ID MSRA2_STAA8 Reviewed; 177 AA.
AC P0A086; Q2FYK8; Q93P63;
DT 15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Peptide methionine sulfoxide reductase MsrA 2;
DE Short=Protein-methionine-S-oxide reductase 2;
DE EC=1.8.4.11;
DE AltName: Full=Peptide-methionine (S)-S-oxide reductase 2;
DE Short=Peptide Met(O) reductase 2;
GN Name=msrA2; Synonyms=msrA; OrderedLocusNames=SAOUHSC_01432;
OS Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=93061;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CATALYTIC ACTIVITY.
RX PubMed=11700354; DOI=10.1099/00221287-147-11-3037;
RA Singh V.K., Moskovitz J., Wilkinson B.J., Jayaswal R.K.;
RT "Molecular characterization of a chromosomal locus in Staphylococcus aureus
RT that contributes to oxidative defence and is highly induced by the cell-
RT wall-active antibiotic oxacillin.";
RL Microbiology 147:3037-3045(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC 8325 / PS 47;
RA Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT "The Staphylococcus aureus NCTC 8325 genome.";
RL (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL D.C. (2006).
RN [3]
RP CATALYTIC ACTIVITY, AND STEREOSPECIFICITY.
RX PubMed=11779133; DOI=10.1006/bbrc.2001.6171;
RA Moskovitz J., Singh V.K., Requena J., Wilkinson B.J., Jayaswal R.K.,
RA Stadtman E.R.;
RT "Purification and characterization of methionine sulfoxide reductases from
RT mouse and Staphylococcus aureus and their substrate stereospecificity.";
RL Biochem. Biophys. Res. Commun. 290:62-65(2002).
CC -!- FUNCTION: Has an important function as a repair enzyme for proteins
CC that have been inactivated by oxidation. Catalyzes the reversible
CC oxidation-reduction of methionine sulfoxide in proteins to methionine
CC (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[thioredoxin]-disulfide + H2O + L-methionyl-[protein] =
CC [thioredoxin]-dithiol + L-methionyl-(S)-S-oxide-[protein];
CC Xref=Rhea:RHEA:14217, Rhea:RHEA-COMP:10698, Rhea:RHEA-COMP:10700,
CC Rhea:RHEA-COMP:12313, Rhea:RHEA-COMP:12315, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:16044, ChEBI:CHEBI:29950, ChEBI:CHEBI:44120,
CC ChEBI:CHEBI:50058; EC=1.8.4.11;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[thioredoxin]-disulfide + H2O + L-methionine = [thioredoxin]-
CC dithiol + L-methionine (S)-S-oxide; Xref=Rhea:RHEA:19993, Rhea:RHEA-
CC COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:29950, ChEBI:CHEBI:50058, ChEBI:CHEBI:57844,
CC ChEBI:CHEBI:58772; EC=1.8.4.11;
CC -!- INDUCTION: Induced by oxacillin but not by hydrogen peroxide.
CC -!- MISCELLANEOUS: Stereospecific for the S isomer of MetO.
CC -!- SIMILARITY: Belongs to the MsrA Met sulfoxide reductase family.
CC {ECO:0000305}.
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DR EMBL; AF349112; AAK83251.1; -; Genomic_DNA.
DR EMBL; CP000253; ABD30524.1; -; Genomic_DNA.
DR RefSeq; WP_000159902.1; NZ_LS483365.1.
DR RefSeq; YP_499957.1; NC_007795.1.
DR AlphaFoldDB; P0A086; -.
DR SMR; P0A086; -.
DR STRING; 1280.SAXN108_1445; -.
DR EnsemblBacteria; ABD30524; ABD30524; SAOUHSC_01432.
DR GeneID; 3920213; -.
DR KEGG; sao:SAOUHSC_01432; -.
DR PATRIC; fig|93061.5.peg.1308; -.
DR eggNOG; COG0225; Bacteria.
DR HOGENOM; CLU_031040_10_1_9; -.
DR OMA; AGPFYYA; -.
DR PHI-base; PHI:4580; -.
DR PRO; PR:P0A086; -.
DR Proteomes; UP000008816; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0036456; F:L-methionine-(S)-S-oxide reductase activity; IBA:GO_Central.
DR GO; GO:0008113; F:peptide-methionine (S)-S-oxide reductase activity; IBA:GO_Central.
DR GO; GO:0034599; P:cellular response to oxidative stress; IBA:GO_Central.
DR Gene3D; 3.30.1060.10; -; 1.
DR HAMAP; MF_01401; MsrA; 1.
DR InterPro; IPR002569; Met_Sox_Rdtase_MsrA_dom.
DR InterPro; IPR036509; Met_Sox_Rdtase_MsrA_sf.
DR Pfam; PF01625; PMSR; 1.
DR SUPFAM; SSF55068; SSF55068; 1.
DR TIGRFAMs; TIGR00401; msrA; 1.
PE 1: Evidence at protein level;
KW Oxidoreductase; Reference proteome.
FT CHAIN 1..177
FT /note="Peptide methionine sulfoxide reductase MsrA 2"
FT /id="PRO_0000138590"
FT ACT_SITE 12
FT /evidence="ECO:0000250"
SQ SEQUENCE 177 AA; 20588 MW; 6BE5ABDE1545A5D4 CRC64;
MTKEYATLAG GCFWCMVKPF TSYPGIKSVV SGYSGGHVDN PTYEQVCTNQ TGHVEAVQIT
FDPEVTSFEN ILDIYFKTFD PTDDQGQFFD RGESYQPVIF YHDEHQKKAA EFKKQQLNEQ
GIFKKPVITP IKPYKNFYPA EDYHQDYYKK NPVHYYQYQR GSGRKAFIES HWGNQNA
