MSRA_BRANA
ID MSRA_BRANA Reviewed; 257 AA.
AC P54151;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Peptide methionine sulfoxide reductase;
DE EC=1.8.4.11;
DE AltName: Full=Peptide-methionine (S)-S-oxide reductase;
DE Short=Peptide Met(O) reductase;
DE AltName: Full=Protein-methionine-S-oxide reductase;
GN Name=PMSR;
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Jet neuf;
RX PubMed=8111035; DOI=10.1007/bf00020171;
RA Keddie J.S., Tsiantis M., Piffanelli P., Cella R., Hatzopoulos P.,
RA Murphy D.J.;
RT "A seed-specific Brassica napus oleosin promoter interacts with a G-box-
RT specific protein and may be bi-directional.";
RL Plant Mol. Biol. 24:327-340(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANTS LEU-4; ALA-14; SER-24; ALA-75 AND
RP SER-129.
RC TISSUE=Leaf;
RX PubMed=8771781; DOI=10.1046/j.1365-313x.1996.10020235.x;
RA Sadanandom A.C., Piffanelli P., Knott T., Robinson C., Sharpe A.,
RA Lydiate D., Murphy D.J., Fairbairn D.J.;
RT "Identification of a peptide methionine sulphoxide reductase gene in an
RT oleosin promoter from Brassica napus.";
RL Plant J. 10:235-242(1996).
CC -!- FUNCTION: Has an important function as a repair enzyme for proteins
CC that have been inactivated by oxidation. Catalyzes the reversible
CC oxidation-reduction of methionine sulfoxide in proteins to methionine.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[thioredoxin]-disulfide + H2O + L-methionyl-[protein] =
CC [thioredoxin]-dithiol + L-methionyl-(S)-S-oxide-[protein];
CC Xref=Rhea:RHEA:14217, Rhea:RHEA-COMP:10698, Rhea:RHEA-COMP:10700,
CC Rhea:RHEA-COMP:12313, Rhea:RHEA-COMP:12315, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:16044, ChEBI:CHEBI:29950, ChEBI:CHEBI:44120,
CC ChEBI:CHEBI:50058; EC=1.8.4.11;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[thioredoxin]-disulfide + H2O + L-methionine = [thioredoxin]-
CC dithiol + L-methionine (S)-S-oxide; Xref=Rhea:RHEA:19993, Rhea:RHEA-
CC COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:29950, ChEBI:CHEBI:50058, ChEBI:CHEBI:57844,
CC ChEBI:CHEBI:58772; EC=1.8.4.11;
CC -!- SIMILARITY: Belongs to the MsrA Met sulfoxide reductase family.
CC {ECO:0000305}.
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DR EMBL; X94225; CAA63919.1; -; Genomic_DNA.
DR EMBL; Z48619; CAA88538.1; -; mRNA.
DR EMBL; X91486; CAA62760.1; -; mRNA.
DR PIR; S55365; S55365.
DR PIR; T47215; T47215.
DR RefSeq; NP_001302774.1; NM_001315845.1.
DR RefSeq; NP_001302784.1; NM_001315855.1.
DR AlphaFoldDB; P54151; -.
DR SMR; P54151; -.
DR GeneID; 106346565; -.
DR GeneID; 106442570; -.
DR KEGG; bna:106346565; -.
DR KEGG; bna:106442570; -.
DR GO; GO:0033744; F:L-methionine:thioredoxin-disulfide S-oxidoreductase activity; IEA:RHEA.
DR GO; GO:0008113; F:peptide-methionine (S)-S-oxide reductase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.30.1060.10; -; 1.
DR HAMAP; MF_01401; MsrA; 1.
DR InterPro; IPR002569; Met_Sox_Rdtase_MsrA_dom.
DR InterPro; IPR036509; Met_Sox_Rdtase_MsrA_sf.
DR Pfam; PF01625; PMSR; 1.
DR SUPFAM; SSF55068; SSF55068; 1.
DR TIGRFAMs; TIGR00401; msrA; 1.
PE 2: Evidence at transcript level;
KW Oxidoreductase; Phosphoprotein.
FT CHAIN 1..257
FT /note="Peptide methionine sulfoxide reductase"
FT /id="PRO_0000138633"
FT REGION 61..88
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 244
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LY15"
FT VARIANT 4
FT /note="I -> L"
FT /evidence="ECO:0000269|PubMed:8771781"
FT VARIANT 14
FT /note="V -> A"
FT /evidence="ECO:0000269|PubMed:8771781"
FT VARIANT 24
FT /note="A -> S"
FT /evidence="ECO:0000269|PubMed:8771781"
FT VARIANT 75
FT /note="S -> A"
FT /evidence="ECO:0000269|PubMed:8771781"
FT VARIANT 129
FT /note="T -> S"
FT /evidence="ECO:0000269|PubMed:8771781"
SQ SEQUENCE 257 AA; 28466 MW; 41F4440E41772788 CRC64;
MLSIVASPPV ISAVSLSKPL QSLAKAALSL SKRAKPTSPF PKTARSISVY KSPMNNLFTR
LGFGSRPQPD PAASSAIAQG PDDDVPSPGQ QFAQFGAGCF WGAELAYQRV PGVTKTEVGY
SHGFVDNPTY EDVCSETTGH NEIVRVQYDP KEVSFESLLD VFWKRHDPTT LNRQGNDVGT
RYRSGIYFYT DEQEKLAREA MEKQQKILNR KIVTEILPAT KFYRAENYHQ QYLAKGGRMG
LSQSAEKGCN DPIRCYG
