708A6_MAIZE
ID 708A6_MAIZE Reviewed; 475 AA.
AC A0A096SRM5; B4FHI9;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 26-NOV-2014, sequence version 1.
DT 03-AUG-2022, entry version 40.
DE RecName: Full=UDP-glycosyltransferase 708A6 {ECO:0000303|PubMed:24045947};
DE EC=2.4.1.- {ECO:0000305};
GN Name=UGT708A6 {ECO:0000303|PubMed:24045947};
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. B73;
RX PubMed=19965430; DOI=10.1126/science.1178534;
RA Schnable P.S., Ware D., Fulton R.S., Stein J.C., Wei F., Pasternak S.,
RA Liang C., Zhang J., Fulton L., Graves T.A., Minx P., Reily A.D.,
RA Courtney L., Kruchowski S.S., Tomlinson C., Strong C., Delehaunty K.,
RA Fronick C., Courtney B., Rock S.M., Belter E., Du F., Kim K., Abbott R.M.,
RA Cotton M., Levy A., Marchetto P., Ochoa K., Jackson S.M., Gillam B.,
RA Chen W., Yan L., Higginbotham J., Cardenas M., Waligorski J., Applebaum E.,
RA Phelps L., Falcone J., Kanchi K., Thane T., Scimone A., Thane N., Henke J.,
RA Wang T., Ruppert J., Shah N., Rotter K., Hodges J., Ingenthron E.,
RA Cordes M., Kohlberg S., Sgro J., Delgado B., Mead K., Chinwalla A.,
RA Leonard S., Crouse K., Collura K., Kudrna D., Currie J., He R.,
RA Angelova A., Rajasekar S., Mueller T., Lomeli R., Scara G., Ko A.,
RA Delaney K., Wissotski M., Lopez G., Campos D., Braidotti M., Ashley E.,
RA Golser W., Kim H., Lee S., Lin J., Dujmic Z., Kim W., Talag J., Zuccolo A.,
RA Fan C., Sebastian A., Kramer M., Spiegel L., Nascimento L., Zutavern T.,
RA Miller B., Ambroise C., Muller S., Spooner W., Narechania A., Ren L.,
RA Wei S., Kumari S., Faga B., Levy M.J., McMahan L., Van Buren P.,
RA Vaughn M.W., Ying K., Yeh C.-T., Emrich S.J., Jia Y., Kalyanaraman A.,
RA Hsia A.-P., Barbazuk W.B., Baucom R.S., Brutnell T.P., Carpita N.C.,
RA Chaparro C., Chia J.-M., Deragon J.-M., Estill J.C., Fu Y., Jeddeloh J.A.,
RA Han Y., Lee H., Li P., Lisch D.R., Liu S., Liu Z., Nagel D.H., McCann M.C.,
RA SanMiguel P., Myers A.M., Nettleton D., Nguyen J., Penning B.W.,
RA Ponnala L., Schneider K.L., Schwartz D.C., Sharma A., Soderlund C.,
RA Springer N.M., Sun Q., Wang H., Waterman M., Westerman R., Wolfgruber T.K.,
RA Yang L., Yu Y., Zhang L., Zhou S., Zhu Q., Bennetzen J.L., Dawe R.K.,
RA Jiang J., Jiang N., Presting G.G., Wessler S.R., Aluru S.,
RA Martienssen R.A., Clifton S.W., McCombie W.R., Wing R.A., Wilson R.K.;
RT "The B73 maize genome: complexity, diversity, and dynamics.";
RL Science 326:1112-1115(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. B73;
RX PubMed=19936069; DOI=10.1371/journal.pgen.1000740;
RA Soderlund C., Descour A., Kudrna D., Bomhoff M., Boyd L., Currie J.,
RA Angelova A., Collura K., Wissotski M., Ashley E., Morrow D., Fernandes J.,
RA Walbot V., Yu Y.;
RT "Sequencing, mapping, and analysis of 27,455 maize full-length cDNAs.";
RL PLoS Genet. 5:E1000740-E1000740(2009).
RN [3]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=24045947; DOI=10.1074/jbc.m113.510040;
RA Falcone Ferreyra M.L., Rodriguez E., Casas M.I., Labadie G., Grotewold E.,
RA Casati P.;
RT "Identification of a bifunctional maize C- and O-glucosyltransferase.";
RL J. Biol. Chem. 288:31678-31688(2013).
CC -!- FUNCTION: Bifunctional glycosyltransferase that can produce both C- and
CC O-glycosidated flavonoids. Converts 2-hydroxynaringenin to isovitexin.
CC Converts eriodictyol to orientin and isoorientin. Converts naringenin
CC and eriodictyol to naringenin 7-O-glucoside and eriodictyol 7-O-
CC glucoside, respectively. {ECO:0000269|PubMed:24045947}.
CC -!- TISSUE SPECIFICITY: Expressed in radicles, hypocotyls and juvenile
CC leaves. Expressed at low levels in roots.
CC {ECO:0000269|PubMed:24045947}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; BT036577; ACF81582.1; -; mRNA.
DR RefSeq; NP_001132650.2; NM_001139178.2.
DR RefSeq; XP_008647434.1; XM_008649212.1.
DR PDB; 6LF6; X-ray; 2.04 A; A=1-475.
DR PDBsum; 6LF6; -.
DR AlphaFoldDB; A0A096SRM5; -.
DR SMR; A0A096SRM5; -.
DR STRING; 4577.GRMZM2G162783_P01; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR PaxDb; A0A096SRM5; -.
DR EnsemblPlants; Zm00001eb280920_T001; Zm00001eb280920_P001; Zm00001eb280920.
DR GeneID; 100194125; -.
DR Gramene; Zm00001eb280920_T001; Zm00001eb280920_P001; Zm00001eb280920.
DR KEGG; zma:100194125; -.
DR eggNOG; KOG1192; Eukaryota.
DR HOGENOM; CLU_001724_3_1_1; -.
DR OMA; ALHDPKH; -.
DR OrthoDB; 508327at2759; -.
DR Proteomes; UP000007305; Chromosome 6.
DR ExpressionAtlas; A0A096SRM5; baseline and differential.
DR GO; GO:0047243; F:flavanone 7-O-beta-glucosyltransferase activity; IDA:UniProtKB.
DR GO; GO:0035251; F:UDP-glucosyltransferase activity; IDA:UniProtKB.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..475
FT /note="UDP-glycosyltransferase 708A6"
FT /id="PRO_0000434461"
FT BINDING 286
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
FT BINDING 348..349
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
FT BINDING 366..374
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
FT BINDING 388..391
FT /ligand="UDP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:58885"
FT /evidence="ECO:0000250|UniProtKB:Q9M156"
FT CONFLICT 129..131
FT /note="ALP -> VLH (in Ref. 2; ACF81582)"
FT /evidence="ECO:0000305"
FT CONFLICT 396
FT /note="A -> T (in Ref. 2; ACF81582)"
FT /evidence="ECO:0000305"
FT CONFLICT 426
FT /note="K -> N (in Ref. 2; ACF81582)"
FT /evidence="ECO:0000305"
FT CONFLICT 453
FT /note="A -> G (in Ref. 2; ACF81582)"
FT /evidence="ECO:0000305"
FT CONFLICT 471..475
FT /note="DAERK -> K (in Ref. 2; ACF81582)"
FT /evidence="ECO:0000305"
FT STRAND 14..18
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 23..40
FT /evidence="ECO:0007829|PDB:6LF6"
FT STRAND 43..48
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 55..67
FT /evidence="ECO:0007829|PDB:6LF6"
FT TURN 68..71
FT /evidence="ECO:0007829|PDB:6LF6"
FT STRAND 72..76
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 84..86
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 92..102
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 103..106
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 107..114
FT /evidence="ECO:0007829|PDB:6LF6"
FT STRAND 117..122
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 123..125
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 126..136
FT /evidence="ECO:0007829|PDB:6LF6"
FT STRAND 140..144
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 148..163
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 187..189
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 192..195
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 200..211
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 212..214
FT /evidence="ECO:0007829|PDB:6LF6"
FT STRAND 215..222
FT /evidence="ECO:0007829|PDB:6LF6"
FT TURN 224..226
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 228..236
FT /evidence="ECO:0007829|PDB:6LF6"
FT TURN 237..239
FT /evidence="ECO:0007829|PDB:6LF6"
FT STRAND 240..242
FT /evidence="ECO:0007829|PDB:6LF6"
FT STRAND 248..250
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 266..272
FT /evidence="ECO:0007829|PDB:6LF6"
FT STRAND 279..283
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 292..305
FT /evidence="ECO:0007829|PDB:6LF6"
FT STRAND 308..312
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 325..330
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 332..337
FT /evidence="ECO:0007829|PDB:6LF6"
FT TURN 338..341
FT /evidence="ECO:0007829|PDB:6LF6"
FT STRAND 342..347
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 351..356
FT /evidence="ECO:0007829|PDB:6LF6"
FT STRAND 360..365
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 369..378
FT /evidence="ECO:0007829|PDB:6LF6"
FT STRAND 382..384
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 391..400
FT /evidence="ECO:0007829|PDB:6LF6"
FT STRAND 403..406
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 421..433
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 435..452
FT /evidence="ECO:0007829|PDB:6LF6"
FT HELIX 457..470
FT /evidence="ECO:0007829|PDB:6LF6"
SQ SEQUENCE 475 AA; 50603 MW; ABB69BF66575BB20 CRC64;
MAANGGDHTS ARPHVVLLPS AGMGHLVPFA RLAVALSEGH GCNVSVAAVQ PTVSSAESRL
LDALFVAAAP AVRRLDFRLA PFDESEFPGA DPFFLRFEAT RRSAPLLGPL LDAAEASALV
TDIVLASVAL PVARERGVPC YVLFTSSAAM LSLCAYFPAY LDAHAAAGSV GVGVGNVDIP
GVFRIPKSSV PQALHDPDHL FTQQFVANGR CLVACDGILV NTFDAFEPDA VTALRQGSIT
VSGGFPPVFT VGPMLPVRFQ AEETADYMRW LSAQPPRSVV YVSFGSRKAI PRDQLRELAA
GLEASGKRFL WVVKSTIVDR DDTADLGGLL GDGFLERVQG RAFVTMGWVE QEEILQHGSV
GLFISHCGWN SLTEAAAFGV PVLAWPRFGD QRVNAALVAR SGLGAWEEGW TWDGEEGLTT
RKEVAKKIKG MMGYDAVAEK AAKVGDAAAA AIAKCGTSYQ SLEEFVQRCR DAERK
