MSSP2_ARATH
ID MSSP2_ARATH Reviewed; 729 AA.
AC Q8LPQ8; O65497;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2006, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Monosaccharide-sensing protein 2;
DE AltName: Full=Sugar transporter MSSP2;
GN Name=MSSP2; OrderedLocusNames=At4g35300; ORFNames=F23E12.140;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Stamme C., Tjaden J., Trentmann O., Emmerlich V., Neuhaus E.;
RT "A novel family of plant monosaccharide transporters is involved in a new
RT type of eukaryotic sugar sensing.";
RL Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY.
RX PubMed=16923188; DOI=10.1186/1471-2148-6-64;
RA Johnson D.A., Hill J.P., Thomas M.A.;
RT "The monosaccharide transporter gene family in land plants is ancient and
RT shows differential subfamily expression and expansion across lineages.";
RL BMC Evol. Biol. 6:64-64(2006).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448 (ISOFORM 2), AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8LPQ8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8LPQ8-2; Sequence=VSP_021553;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to an intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sugar
CC transporter (TC 2.A.1.1) family. {ECO:0000305}.
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DR EMBL; AJ532570; CAD58692.1; -; mRNA.
DR EMBL; AL022604; CAA18739.1; -; Genomic_DNA.
DR EMBL; AL161587; CAB80247.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86493.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86494.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86495.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86497.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66216.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66217.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66218.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66219.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66220.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66221.1; -; Genomic_DNA.
DR EMBL; AY094465; AAM19835.1; -; mRNA.
DR PIR; T06127; T06127.
DR RefSeq; NP_001154287.1; NM_001160815.2. [Q8LPQ8-1]
DR RefSeq; NP_001190923.1; NM_001203994.1. [Q8LPQ8-2]
DR RefSeq; NP_001328125.1; NM_001342339.1. [Q8LPQ8-2]
DR RefSeq; NP_001328126.1; NM_001342340.1. [Q8LPQ8-2]
DR RefSeq; NP_001328127.1; NM_001342343.1. [Q8LPQ8-2]
DR RefSeq; NP_001328128.1; NM_001342344.1. [Q8LPQ8-1]
DR RefSeq; NP_001328129.1; NM_001342341.1. [Q8LPQ8-2]
DR RefSeq; NP_001328130.1; NM_001342342.1. [Q8LPQ8-2]
DR RefSeq; NP_195256.3; NM_119696.5. [Q8LPQ8-1]
DR RefSeq; NP_849565.1; NM_179234.2. [Q8LPQ8-2]
DR AlphaFoldDB; Q8LPQ8; -.
DR BioGRID; 14966; 8.
DR IntAct; Q8LPQ8; 5.
DR STRING; 3702.AT4G35300.1; -.
DR TCDB; 2.A.1.1.84; the major facilitator superfamily (mfs).
DR iPTMnet; Q8LPQ8; -.
DR SwissPalm; Q8LPQ8; -.
DR PaxDb; Q8LPQ8; -.
DR PRIDE; Q8LPQ8; -.
DR EnsemblPlants; AT4G35300.1; AT4G35300.1; AT4G35300. [Q8LPQ8-2]
DR EnsemblPlants; AT4G35300.10; AT4G35300.10; AT4G35300. [Q8LPQ8-2]
DR EnsemblPlants; AT4G35300.11; AT4G35300.11; AT4G35300. [Q8LPQ8-2]
DR EnsemblPlants; AT4G35300.2; AT4G35300.2; AT4G35300. [Q8LPQ8-1]
DR EnsemblPlants; AT4G35300.3; AT4G35300.3; AT4G35300. [Q8LPQ8-1]
DR EnsemblPlants; AT4G35300.4; AT4G35300.4; AT4G35300. [Q8LPQ8-2]
DR EnsemblPlants; AT4G35300.6; AT4G35300.6; AT4G35300. [Q8LPQ8-2]
DR EnsemblPlants; AT4G35300.7; AT4G35300.7; AT4G35300. [Q8LPQ8-2]
DR EnsemblPlants; AT4G35300.8; AT4G35300.8; AT4G35300. [Q8LPQ8-2]
DR EnsemblPlants; AT4G35300.9; AT4G35300.9; AT4G35300. [Q8LPQ8-1]
DR GeneID; 829684; -.
DR Gramene; AT4G35300.1; AT4G35300.1; AT4G35300. [Q8LPQ8-2]
DR Gramene; AT4G35300.10; AT4G35300.10; AT4G35300. [Q8LPQ8-2]
DR Gramene; AT4G35300.11; AT4G35300.11; AT4G35300. [Q8LPQ8-2]
DR Gramene; AT4G35300.2; AT4G35300.2; AT4G35300. [Q8LPQ8-1]
DR Gramene; AT4G35300.3; AT4G35300.3; AT4G35300. [Q8LPQ8-1]
DR Gramene; AT4G35300.4; AT4G35300.4; AT4G35300. [Q8LPQ8-2]
DR Gramene; AT4G35300.6; AT4G35300.6; AT4G35300. [Q8LPQ8-2]
DR Gramene; AT4G35300.7; AT4G35300.7; AT4G35300. [Q8LPQ8-2]
DR Gramene; AT4G35300.8; AT4G35300.8; AT4G35300. [Q8LPQ8-2]
DR Gramene; AT4G35300.9; AT4G35300.9; AT4G35300. [Q8LPQ8-1]
DR KEGG; ath:AT4G35300; -.
DR Araport; AT4G35300; -.
DR TAIR; locus:2122138; AT4G35300.
DR eggNOG; KOG0254; Eukaryota.
DR InParanoid; Q8LPQ8; -.
DR OMA; VVCVIAW; -.
DR PhylomeDB; Q8LPQ8; -.
DR PRO; PR:Q8LPQ8; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8LPQ8; baseline and differential.
DR Genevisible; Q8LPQ8; AT.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR003663; Sugar/inositol_transpt.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF00083; Sugar_tr; 2.
DR PRINTS; PR00171; SUGRTRNSPORT.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Phosphoprotein; Reference proteome;
KW Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..729
FT /note="Monosaccharide-sensing protein 2"
FT /id="PRO_0000259883"
FT TRANSMEM 1..21
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 507..527
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 553..573
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 585..605
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 610..630
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 650..670
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 679..699
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT REGION 347..367
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 423..442
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 438
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96290"
FT VAR_SEQ 432
FT /note="E -> EGGFKRIYLHQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_021553"
FT MOD_RES Q8LPQ8-2:448
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19245862"
SQ SEQUENCE 729 AA; 78525 MW; 81A35DDF8725411E CRC64;
MSGAVLVAIA AAVGNLLQGW DNATIAGAVL YIKKEFNLES NPSVEGLIVA MSLIGATLIT
TCSGGVADWL GRRPMLILSS ILYFVGSLVM LWSPNVYVLL LGRLLDGFGV GLVVTLVPIY
ISETAPPEIR GLLNTLPQFT GSGGMFLSYC MVFGMSLMPS PSWRLMLGVL FIPSLVFFFL
TVFFLPESPR WLVSKGRMLE AKRVLQRLRG REDVSGEMAL LVEGLGIGGE TTIEEYIIGP
ADEVTDDHDI AVDKDQIKLY GAEEGLSWVA RPVKGGSTMS VLSRHGSTMS RRQGSLIDPL
VTLFGSVHEK MPDTGSMRSA LFPHFGSMFS VGGNQPRHED WDEENLVGEG EDYPSDHGDD
SEDDLHSPLI SRQTTSMEKD MPHTAHGTLS TFRHGSQVQG AQGEGAGSMG IGGGWQVAWK
WTEREDESGQ KEEGFPGSRR GSIVSLPGGD GTGEADFVQA SALVSQPALY SKDLLKEHTI
GPAMVHPSET TKGSIWHDLH DPGVKRALVV GVGLQILQQF SGINGVLYYT PQILEQAGVG
ILLSNMGISS SSASLLISAL TTFVMLPAIA VAMRLMDLSG RRTLLLTTIP ILIASLLVLV
ISNLVHMNSI VHAVLSTVSV VLYFCFFVMG FGPAPNILCS EIFPTRVRGI CIAICALTFW
ICDIIVTYSL PVLLKSIGLA GVFGMYAIVC CISWVFVFIK VPETKGMPLE VITEFFSVGA
RQAEAAKNE
