MSX1_MOUSE
ID MSX1_MOUSE Reviewed; 303 AA.
AC P13297; Q91W75; Q9ERF6;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2013, sequence version 4.
DT 03-AUG-2022, entry version 203.
DE RecName: Full=Homeobox protein MSX-1;
DE AltName: Full=Homeobox protein Hox-7;
DE AltName: Full=Hox-7.1;
DE AltName: Full=Msh homeobox 1-like protein;
GN Name=Msx1; Synonyms=Hox7, Hox7.1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Embryo;
RX PubMed=2565278; DOI=10.1101/gad.3.1.26;
RA Hill R.E., Jones P.F., Rees A.R., Sime C.M., Justice M.J., Copeland N.G.,
RA Jenkins N.A., Graham E., Davidson D.R.;
RT "A new family of mouse homeo box-containing genes: molecular structure,
RT chromosomal location, and developmental expression of Hox-7.1.";
RL Genes Dev. 3:26-37(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=1682128; DOI=10.1242/dev.112.4.1053;
RA Monaghan A.P., Davidson D.R., Sime C., Graham E., Baldock R.,
RA Bhattacharya S.S., Hill R.E.;
RT "The Msh-like homeobox genes define domains in the developing vertebrate
RT eye.";
RL Development 112:1053-1061(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RA Wu X.B., Liu Y.-H.;
RT "Isolation of a mouse homeodomain protein MSX1 cDNA variant.";
RL Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 158-256.
RX PubMed=2565810; DOI=10.1002/j.1460-2075.1989.tb03352.x;
RA Robert B., Sassoon D., Jacq B., Gehring W.J., Buckingham M.;
RT "Hox-7, a mouse homeobox gene with a novel pattern of expression during
RT embryogenesis.";
RL EMBO J. 8:91-100(1989).
RN [8]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 171-231.
RX PubMed=1673109; DOI=10.1016/0378-1119(91)90182-b;
RA Holland P.W.H.;
RT "Cloning and evolutionary analysis of msh-like homeobox genes from mouse,
RT zebrafish and ascidian.";
RL Gene 98:253-257(1991).
RN [9]
RP FUNCTION.
RX PubMed=1677742; DOI=10.1038/352429a0;
RA Davidson D.R., Crawley A., Hill R.E., Tickle C.;
RT "Position-dependent expression of two related homeobox genes in developing
RT vertebrate limbs.";
RL Nature 352:429-431(1991).
RN [10]
RP FUNCTION.
RX PubMed=7823952; DOI=10.1128/mcb.15.2.861;
RA Catron K.M., Zhang H., Marshall S.C., Inostroza J.A., Wilson J.M.,
RA Abate C.;
RT "Transcriptional repression by Msx-1 does not require homeodomain DNA-
RT binding sites.";
RL Mol. Cell. Biol. 15:861-871(1995).
RN [11]
RP SUMOYLATION AT LYS-15 AND LYS-133, AND MUTAGENESIS OF LYS-15; LYS-53;
RP LYS-72; LYS-125; LYS-133 AND LYS-139.
RX PubMed=16678795; DOI=10.1016/j.bbrc.2006.03.232;
RA Gupta V., Bei M.;
RT "Modification of Msx1 by SUMO-1.";
RL Biochem. Biophys. Res. Commun. 345:74-77(2006).
RN [12]
RP X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 173-230 IN COMPLEX WITH DNA.
RX PubMed=11580277; DOI=10.1021/bi0108148;
RA Hovde S., Abate-Shen C., Geiger J.H.;
RT "Crystal structure of the Msx-1 homeodomain/DNA complex.";
RL Biochemistry 40:12013-12021(2001).
CC -!- FUNCTION: Acts as a transcriptional repressor. May play a role in limb-
CC pattern formation. Acts in cranofacial development and specifically in
CC odontogenesis. {ECO:0000269|PubMed:1677742,
CC ECO:0000269|PubMed:7823952}.
CC -!- INTERACTION:
CC P13297; P43276: H1-5; NbExp=3; IntAct=EBI-903969, EBI-903960;
CC P13297; P25233: Ndn; NbExp=2; IntAct=EBI-903969, EBI-1801080;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- PTM: Sumoylated by PIAS1, desumoylated by SENP1.
CC {ECO:0000269|PubMed:16678795}.
CC -!- SIMILARITY: Belongs to the Msh homeobox family. {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-7 is the initiator.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH16426.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAA32871.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAA32871.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X14759; CAA32871.1; ALT_SEQ; mRNA.
DR EMBL; X59251; CAA41944.1; -; mRNA.
DR EMBL; AF308572; AAG32466.1; -; mRNA.
DR EMBL; AC114671; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466524; EDL37531.1; -; Genomic_DNA.
DR EMBL; BC016426; AAH16426.1; ALT_INIT; mRNA.
DR EMBL; X14457; CAA32626.1; -; Genomic_DNA.
DR EMBL; M38575; AAA37823.1; -; Genomic_DNA.
DR CCDS; CCDS19249.1; -.
DR PIR; PS0411; PS0411.
DR PIR; S03636; S03636.
DR PIR; S18813; S18813.
DR RefSeq; NP_034965.2; NM_010835.2.
DR PDB; 1IG7; X-ray; 2.20 A; A=173-230.
DR PDBsum; 1IG7; -.
DR AlphaFoldDB; P13297; -.
DR SMR; P13297; -.
DR BioGRID; 201536; 12.
DR IntAct; P13297; 5.
DR STRING; 10090.ENSMUSP00000058354; -.
DR iPTMnet; P13297; -.
DR PhosphoSitePlus; P13297; -.
DR PaxDb; P13297; -.
DR PRIDE; P13297; -.
DR ProteomicsDB; 290102; -.
DR Antibodypedia; 9270; 396 antibodies from 41 providers.
DR DNASU; 17701; -.
DR Ensembl; ENSMUST00000063116; ENSMUSP00000058354; ENSMUSG00000048450.
DR GeneID; 17701; -.
DR KEGG; mmu:17701; -.
DR UCSC; uc008xfw.1; mouse.
DR CTD; 4487; -.
DR MGI; MGI:97168; Msx1.
DR VEuPathDB; HostDB:ENSMUSG00000048450; -.
DR eggNOG; KOG0492; Eukaryota.
DR GeneTree; ENSGT00940000161623; -.
DR HOGENOM; CLU_072675_1_0_1; -.
DR InParanoid; P13297; -.
DR OMA; MQTPRFS; -.
DR OrthoDB; 1226077at2759; -.
DR PhylomeDB; P13297; -.
DR TreeFam; TF350699; -.
DR BioGRID-ORCS; 17701; 2 hits in 73 CRISPR screens.
DR EvolutionaryTrace; P13297; -.
DR PRO; PR:P13297; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; P13297; protein.
DR Bgee; ENSMUSG00000048450; Expressed in head mesenchyme and 343 other tissues.
DR Genevisible; P13297; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IGI:MGI.
DR GO; GO:0005667; C:transcription regulator complex; IPI:MGI.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:MGI.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0002039; F:p53 binding; ISO:MGI.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:MGI.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0090427; P:activation of meiosis; IGI:MGI.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IGI:MGI.
DR GO; GO:0030509; P:BMP signaling pathway; IDA:MGI.
DR GO; GO:0061312; P:BMP signaling pathway involved in heart development; IGI:MGI.
DR GO; GO:0060349; P:bone morphogenesis; IMP:MGI.
DR GO; GO:0051216; P:cartilage development; ISO:MGI.
DR GO; GO:0060536; P:cartilage morphogenesis; IMP:MGI.
DR GO; GO:0000902; P:cell morphogenesis; ISO:MGI.
DR GO; GO:0042733; P:embryonic digit morphogenesis; IGI:MGI.
DR GO; GO:0035115; P:embryonic forelimb morphogenesis; IGI:MGI.
DR GO; GO:0035116; P:embryonic hindlimb morphogenesis; IGI:MGI.
DR GO; GO:0030326; P:embryonic limb morphogenesis; IGI:MGI.
DR GO; GO:0048598; P:embryonic morphogenesis; IBA:GO_Central.
DR GO; GO:0035880; P:embryonic nail plate morphogenesis; IMP:BHF-UCL.
DR GO; GO:0001837; P:epithelial to mesenchymal transition; IMP:UniProtKB.
DR GO; GO:0003198; P:epithelial to mesenchymal transition involved in endocardial cushion formation; IGI:MGI.
DR GO; GO:0060325; P:face morphogenesis; IMP:BHF-UCL.
DR GO; GO:0030900; P:forebrain development; IMP:MGI.
DR GO; GO:0007507; P:heart development; IGI:MGI.
DR GO; GO:0003007; P:heart morphogenesis; IGI:MGI.
DR GO; GO:0001701; P:in utero embryonic development; IMP:MGI.
DR GO; GO:0061180; P:mammary gland epithelium development; IGI:MGI.
DR GO; GO:0097152; P:mesenchymal cell apoptotic process; IGI:MGI.
DR GO; GO:0010463; P:mesenchymal cell proliferation; IMP:UniProtKB.
DR GO; GO:0030901; P:midbrain development; IMP:MGI.
DR GO; GO:0042474; P:middle ear morphogenesis; IMP:MGI.
DR GO; GO:0007517; P:muscle organ development; IPI:MGI.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IGI:MGI.
DR GO; GO:0030308; P:negative regulation of cell growth; ISO:MGI.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IGI:MGI.
DR GO; GO:0043392; P:negative regulation of DNA binding; IDA:MGI.
DR GO; GO:0051154; P:negative regulation of striated muscle cell differentiation; IDA:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:2000678; P:negative regulation of transcription regulatory region DNA binding; IGI:MGI.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:MGI.
DR GO; GO:0042476; P:odontogenesis; IMP:UniProtKB.
DR GO; GO:0042475; P:odontogenesis of dentin-containing tooth; IMP:BHF-UCL.
DR GO; GO:0030513; P:positive regulation of BMP signaling pathway; IGI:MGI.
DR GO; GO:1902255; P:positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator; ISO:MGI.
DR GO; GO:2001055; P:positive regulation of mesenchymal cell apoptotic process; IGI:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:MGI.
DR GO; GO:0034504; P:protein localization to nucleus; ISO:MGI.
DR GO; GO:0050821; P:protein stabilization; ISO:MGI.
DR GO; GO:0042481; P:regulation of odontogenesis; IGI:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0060021; P:roof of mouth development; IMP:MGI.
DR GO; GO:0023019; P:signal transduction involved in regulation of gene expression; IGI:MGI.
DR GO; GO:0048863; P:stem cell differentiation; IGI:MGI.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IMP:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Developmental protein; DNA-binding; Homeobox;
KW Isopeptide bond; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation; Ubl conjugation.
FT CHAIN 1..303
FT /note="Homeobox protein MSX-1"
FT /id="PRO_0000049091"
FT DNA_BIND 172..231
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 22..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 90..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 135..163
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 15
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO)"
FT CROSSLNK 133
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO)"
FT MUTAGEN 15
FT /note="K->R: Abolishes sumoylation."
FT /evidence="ECO:0000269|PubMed:16678795"
FT MUTAGEN 53
FT /note="K->R: Sumoylated."
FT /evidence="ECO:0000269|PubMed:16678795"
FT MUTAGEN 72
FT /note="K->R: Sumoylated."
FT /evidence="ECO:0000269|PubMed:16678795"
FT MUTAGEN 125
FT /note="K->R: Sumoylated."
FT /evidence="ECO:0000269|PubMed:16678795"
FT MUTAGEN 133
FT /note="K->R: Abolishes sumoylation."
FT /evidence="ECO:0000269|PubMed:16678795"
FT MUTAGEN 139
FT /note="K->R: Sumoylated."
FT /evidence="ECO:0000269|PubMed:16678795"
FT CONFLICT 263..266
FT /note="Missing (in Ref. 2; CAA41944)"
FT /evidence="ECO:0000305"
FT HELIX 181..193
FT /evidence="ECO:0007829|PDB:1IG7"
FT HELIX 199..208
FT /evidence="ECO:0007829|PDB:1IG7"
FT HELIX 213..229
FT /evidence="ECO:0007829|PDB:1IG7"
SQ SEQUENCE 303 AA; 31672 MW; 8E07FF038AEF0EDF CRC64;
MAPAAAMTSL PLGVKVEDSA FAKPAGGGVG QAPGAAAATA TAMGTDEEGA KPKVPASLLP
FSVEALMADH RKPGAKESVL VASEGAQAAG GSVQHLGTRP GSLGAPDAPS SPRPLGHFSV
GGLLKLPEDA LVKAESPEKL DRTPWMQSPR FSPPPARRLS PPACTLRKHK TNRKPRTPFT
TAQLLALERK FRQKQYLSIA ERAEFSSSLS LTETQVKIWF QNRRAKAKRL QEAELEKLKM
AAKPMLPPAA FGLSFPLGGP AAVAAAAGAS LYSASGPFQR AALPVAPVGL YTAHVGYSMY
HLT
