ID   MSX2_BOVIN              Reviewed;         267 AA.
AC   Q0P5C3;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Homeobox protein MSX-2;
GN   Name=MSX2;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Placenta;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Acts as a transcriptional regulator in bone development.
CC       Represses the ALPL promoter activity and antagonizes the stimulatory
CC       effect of DLX5 on ALPL expression during osteoblast differentiation.
CC       Probable morphogenetic role. May play a role in limb-pattern formation.
CC       In osteoblasts, suppresses transcription driven by the osteocalcin FGF
CC       response element (OCFRE). Binds to the homeodomain-response element of
CC       the ALPL promoter (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with MINT, with XRCC6 (Ku70) and XRCC5 (Ku80).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC   -!- SIMILARITY: Belongs to the Msh homeobox family. {ECO:0000305}.
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DR   EMBL; BC120248; AAI20249.1; -; mRNA.
DR   RefSeq; NP_001073082.1; NM_001079614.2.
DR   AlphaFoldDB; Q0P5C3; -.
DR   SMR; Q0P5C3; -.
DR   STRING; 9913.ENSBTAP00000018417; -.
DR   PaxDb; Q0P5C3; -.
DR   Ensembl; ENSBTAT00000018417; ENSBTAP00000018417; ENSBTAG00000013873.
DR   GeneID; 540230; -.
DR   KEGG; bta:540230; -.
DR   CTD; 4488; -.
DR   VEuPathDB; HostDB:ENSBTAG00000013873; -.
DR   VGNC; VGNC:31712; MSX2.
DR   eggNOG; KOG0492; Eukaryota.
DR   GeneTree; ENSGT00940000159824; -.
DR   HOGENOM; CLU_072675_0_0_1; -.
DR   InParanoid; Q0P5C3; -.
DR   OMA; MSPTACT; -.
DR   OrthoDB; 1226077at2759; -.
DR   TreeFam; TF350699; -.
DR   Proteomes; UP000009136; Chromosome 20.
DR   Bgee; ENSBTAG00000013873; Expressed in conceptus and 66 other tissues.
DR   GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR   GO; GO:0016607; C:nuclear speck; IEA:Ensembl.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; ISS:UniProtKB.
DR   GO; GO:0048598; P:embryonic morphogenesis; IBA:GO_Central.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0001649; P:osteoblast differentiation; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR020479; Homeobox_metazoa.
DR   Pfam; PF00046; Homeodomain; 1.
DR   PRINTS; PR00024; HOMEOBOX.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Osteogenesis;
KW   Reference proteome; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..267
FT                   /note="Homeobox protein MSX-2"
FT                   /id="PRO_0000284076"
FT   DNA_BIND        142..201
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          1..74
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          105..147
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   267 AA;  29027 MW;  DB4F56EDFCEF1DDC CRC64;
     MASPSKGNDL FSSDEEGPAM VAGPGPGPGG AEGAAEERRV KVSSLPFSVE ALMSDKKPPK
     ETSPRPAESA SAGATLRPLL LPGHGVREAH SPGPLVKPFE TASVKSENSE DGAAWMQEPG
     RYSPPPRHMS PTTCTLRKHK TNRKPRTPFT TSQLLALERK FRQKQYLSIA ERAEFSSSLN
     LTETQVKIWF QNRRAKAKRL QEAELEKLKM AAKPMLPSGF SLPFPINSPL QAASLYGASY
     PFHRPVLPIP PVGLYATPVG YGMYHLS