ID   MSX2_CANLF              Reviewed;         267 AA.
AC   Q9GK08;
DT   16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 140.
DE   RecName: Full=Homeobox protein MSX-2;
DE   AltName: Full=Homeobox protein Hox-8;
GN   Name=MSX2;
OS   Canis lupus familiaris (Dog) (Canis familiaris).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
OX   NCBI_TaxID=9615;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Haworth K.E., Edwards Y.H.;
RT   "The homeodomain Msx2 protein sequence is highly conserved between between
RT   domestic dog breeds of varying face and skull types.";
RL   Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11419342; DOI=10.1046/j.1365-2052.2001.00702.x;
RA   Haworth K., Breen M., Binns M., Hopkinson D.A., Edwards Y.H.;
RT   "The canine homeobox gene MSX2: sequence, chromosome assignment and genetic
RT   analysis in dogs of different breeds.";
RL   Anim. Genet. 32:32-36(2001).
CC   -!- FUNCTION: Acts as a transcriptional regulator in bone development.
CC       Represses the ALPL promoter activity and antagonizes the stimulatory
CC       effect of DLX5 on ALPL expression during osteoblast differentiation.
CC       Probable morphogenetic role. May play a role in limb-pattern formation.
CC       In osteoblasts, suppresses transcription driven by the osteocalcin FGF
CC       response element (OCFRE). Binds to the homeodomain-response element of
CC       the ALPL promoter (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with MINT, with XRCC6 (Ku70) and XRCC5 (Ku80).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC   -!- SIMILARITY: Belongs to the Msh homeobox family. {ECO:0000305}.
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DR   EMBL; AJ277753; CAC19897.1; -; mRNA.
DR   EMBL; AJ303104; CAC37368.1; -; Genomic_DNA.
DR   EMBL; AJ303105; CAC37368.1; JOINED; Genomic_DNA.
DR   RefSeq; NP_001003098.1; NM_001003098.2.
DR   AlphaFoldDB; Q9GK08; -.
DR   SMR; Q9GK08; -.
DR   STRING; 9612.ENSCAFP00000041488; -.
DR   PaxDb; Q9GK08; -.
DR   Ensembl; ENSCAFT00030003789; ENSCAFP00030003362; ENSCAFG00030002082.
DR   Ensembl; ENSCAFT00040034730; ENSCAFP00040030240; ENSCAFG00040018772.
DR   Ensembl; ENSCAFT00845000718; ENSCAFP00845000523; ENSCAFG00845000450.
DR   GeneID; 403663; -.
DR   KEGG; cfa:403663; -.
DR   CTD; 4488; -.
DR   VEuPathDB; HostDB:ENSCAFG00845000450; -.
DR   eggNOG; KOG0492; Eukaryota.
DR   GeneTree; ENSGT00940000159824; -.
DR   HOGENOM; CLU_072675_0_0_1; -.
DR   InParanoid; Q9GK08; -.
DR   OMA; MSPTACT; -.
DR   OrthoDB; 1226077at2759; -.
DR   TreeFam; TF350699; -.
DR   Proteomes; UP000002254; Chromosome 4.
DR   Bgee; ENSCAFG00000016731; Expressed in placenta and 29 other tissues.
DR   GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR   GO; GO:0016607; C:nuclear speck; IEA:Ensembl.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; ISS:UniProtKB.
DR   GO; GO:0048598; P:embryonic morphogenesis; IBA:GO_Central.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0001649; P:osteoblast differentiation; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR020479; Homeobox_metazoa.
DR   Pfam; PF00046; Homeodomain; 1.
DR   PRINTS; PR00024; HOMEOBOX.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Osteogenesis;
KW   Reference proteome; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..267
FT                   /note="Homeobox protein MSX-2"
FT                   /id="PRO_0000049098"
FT   DNA_BIND        142..201
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          1..147
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   267 AA;  28609 MW;  F0550CCBE9F10CE0 CRC64;
     MASPSKGSDL FSSDEEGPAA LAGPGPGPGG AEGAAEERRV KVSSLPFSVE ALMSDKKPPK
     GASPRPADSA SAGAALRPLL LPGHGAREAP SPGPPGKPFE AASVKSESAE DGAAWMQEPG
     RYSPPPRHMS PTTCTLRKHK TNRKPRTPFT TSQLLALERK FRQKQYLSIA ERAEFSSSLN
     LTETQVKIWF QNRRAKAKRL QEAELEKLKM AAKPMLPSGF SLPFPINSPL QAASIYGASY
     PFHRPVLPIP PVGLYATPVG YGMYHLS