MSX3_MOUSE
ID MSX3_MOUSE Reviewed; 204 AA.
AC P70354; P70246; Q03360; Q80W10;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Homeobox protein MSX-3;
GN Name=Msx3; Synonyms=Msx-3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8887328; DOI=10.1016/s0925-4773(96)00562-x;
RA Wang W., Chen X., Xu H., Lufkin T.;
RT "Msx3: a novel murine homologue of the Drosophila msh homeobox gene
RT restricted to the dorsal embryonic central nervous system.";
RL Mech. Dev. 58:203-215(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=FVB/NHSD;
RA Matsui H., Takahashi T., Raghow R.;
RT "The mechanism of biogenesis, developmental regulation and potential
RT function of the two alternatively spliced mRNAs encoded by the murine Msx3
RT gene.";
RL Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 73-204.
RX PubMed=8861099; DOI=10.1016/0925-4773(96)00505-9;
RA Shimeld S.M., McKay I.J., Sharpe P.T.;
RT "The murine homeobox gene Msx-3 shows highly restricted expression in the
RT developing neural tube.";
RL Mech. Dev. 55:201-210(1996).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 86-146.
RC STRAIN=DBA; TISSUE=Liver;
RX PubMed=1673109; DOI=10.1016/0378-1119(91)90182-b;
RA Holland P.W.H.;
RT "Cloning and evolutionary analysis of msh-like homeobox genes from mouse,
RT zebrafish and ascidian.";
RL Gene 98:253-257(1991).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND SUBUNIT.
RC TISSUE=Muscle;
RX PubMed=11115394;
RA Mehra-Chaudhary R., Matsui H., Raghow R.;
RT "Msx3 protein recruits histone deacetylase to down-regulate the Msx1
RT promoter.";
RL Biochem. J. 353:13-22(2001).
CC -!- FUNCTION: Acts as a potent transcriptional repressor of MSX1.
CC {ECO:0000269|PubMed:11115394}.
CC -!- SUBUNIT: Interacts with CREBBP/CBP, NOTCH1/p300 and HDAC1. Appears to
CC exist in two distinct complexes in the cell, one which includes CREBBP
CC and NOTCH1 and the other which contains HDAC1.
CC {ECO:0000269|PubMed:11115394}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000269|PubMed:11115394}.
CC -!- TISSUE SPECIFICITY: Restricted to the dorsal embryonic central nervous
CC system.
CC -!- SIMILARITY: Belongs to the Msh homeobox family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH51983.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U62523; AAB49935.1; -; mRNA.
DR EMBL; AF060229; AAC15459.1; -; Genomic_DNA.
DR EMBL; BC051983; AAH51983.1; ALT_INIT; mRNA.
DR EMBL; X96518; CAA65367.1; -; Genomic_DNA.
DR EMBL; M38577; AAA37825.1; -; Genomic_DNA.
DR CCDS; CCDS21961.1; -.
DR PIR; PS0409; PS0409.
DR RefSeq; NP_034966.1; NM_010836.3.
DR AlphaFoldDB; P70354; -.
DR SMR; P70354; -.
DR BioGRID; 201538; 1.
DR STRING; 10090.ENSMUSP00000133484; -.
DR PaxDb; P70354; -.
DR PRIDE; P70354; -.
DR DNASU; 17703; -.
DR Ensembl; ENSMUST00000172775; ENSMUSP00000133484; ENSMUSG00000025469.
DR GeneID; 17703; -.
DR KEGG; mmu:17703; -.
DR UCSC; uc012fwf.1; mouse.
DR CTD; 17703; -.
DR MGI; MGI:106587; Msx3.
DR VEuPathDB; HostDB:ENSMUSG00000025469; -.
DR eggNOG; KOG0492; Eukaryota.
DR GeneTree; ENSGT00940000164040; -.
DR HOGENOM; CLU_072675_3_1_1; -.
DR InParanoid; P70354; -.
DR OMA; AAYRMYY; -.
DR OrthoDB; 1197782at2759; -.
DR PhylomeDB; P70354; -.
DR TreeFam; TF350699; -.
DR BioGRID-ORCS; 17703; 0 hits in 71 CRISPR screens.
DR PRO; PR:P70354; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; P70354; protein.
DR Bgee; ENSMUSG00000025469; Expressed in future spinal cord and 36 other tissues.
DR ExpressionAtlas; P70354; baseline and differential.
DR Genevisible; P70354; MM.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0048598; P:embryonic morphogenesis; IBA:GO_Central.
DR GO; GO:0035067; P:negative regulation of histone acetylation; IGI:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR InterPro; IPR000047; HTH_motif.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..204
FT /note="Homeobox protein MSX-3"
FT /id="PRO_0000049104"
FT DNA_BIND 87..146
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 39..89
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 57..78
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 75
FT /note="S -> I (in Ref. 4; CAA65367)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 204 AA; 21971 MW; BC032991DECE4AA1 CRC64;
MARATFDMNA AGLEARGGGH TEHGPLPFSV ESLLEAERVP GSESGELGVE RPLGASKPGA
WPPPVAHSCP PRAPSPPPCT LRKHKTNRKP RTPFTTAQLL ALERKFHQKQ YLSIAERAEF
SSSLSLTETQ VKIWFQNRRA KAKRLQEAEL EKLKLAAKPL LPAAFALPFP LGTQLHSSAA
TFGGNAVPGI LAGPVAAYGM YYLS
