MT1_CALSI
ID MT1_CALSI Reviewed; 59 AA.
AC P55949;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Metallothionein-1B;
DE Short=MT-1B;
DE AltName: Full=Metallothionein-IB;
DE Short=MT-IB;
DE Contains:
DE RecName: Full=Metallothionein-1A;
DE Short=MT-1A;
DE AltName: Full=Metallothionein-IA;
DE Short=MT-IA;
OS Callinectes sapidus (Blue crab).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea;
OC Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Brachyura;
OC Eubrachyura; Portunoidea; Portunidae; Callinectes.
OX NCBI_TaxID=6763;
RN [1]
RP PROTEIN SEQUENCE.
RX PubMed=7487904; DOI=10.1042/bj3110617;
RA Brouwer M., Enghild J., Hoexum-Brouwer T., Thogersen I., Truncali A.;
RT "Primary structure and tissue-specific expression of blue crab (Callinectes
RT sapidus) metallothionein isoforms.";
RL Biochem. J. 311:617-622(1995).
RN [2] {ECO:0007744|PDB:1DMC, ECO:0007744|PDB:1DMD, ECO:0007744|PDB:1DME, ECO:0007744|PDB:1DMF}
RP STRUCTURE BY NMR IN COMPLEX WITH CADMIUM IONS.
RX PubMed=7819257; DOI=10.1021/bi00002a029;
RA Narula S.S., Brouwer M., Hua Y., Armitage I.M.;
RT "Three-dimensional solution structure of Callinectes sapidus
RT metallothionein-1 determined by homonuclear and heteronuclear magnetic
RT resonance spectroscopy.";
RL Biochemistry 34:620-631(1995).
CC -!- FUNCTION: Binds six divalent metal ions. Known to bind copper and
CC cadmium.
CC -!- SIMILARITY: Belongs to the metallothionein superfamily. Type 3 family.
CC {ECO:0000305}.
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DR PIR; S59072; S59072.
DR PDB; 1DMC; NMR; -; A=29-59.
DR PDB; 1DMD; NMR; -; A=29-59.
DR PDB; 1DME; NMR; -; A=2-29.
DR PDB; 1DMF; NMR; -; A=2-29.
DR PDBsum; 1DMC; -.
DR PDBsum; 1DMD; -.
DR PDBsum; 1DME; -.
DR PDBsum; 1DMF; -.
DR AlphaFoldDB; P55949; -.
DR SMR; P55949; -.
DR EvolutionaryTrace; P55949; -.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR InterPro; IPR002045; Metalthion_crustacean.
DR InterPro; IPR017854; Metalthion_dom_sf.
DR PRINTS; PR00858; MTCRUSTACEAN.
DR SUPFAM; SSF57868; SSF57868; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Cadmium; Copper; Direct protein sequencing; Metal-binding;
KW Metal-thiolate cluster.
FT CHAIN 1..59
FT /note="Metallothionein-1B"
FT /id="PRO_0000041754"
FT CHAIN 2..59
FT /note="Metallothionein-1A"
FT /id="PRO_0000041755"
FT REGION 1..29
FT /note="Beta"
FT REGION 30..59
FT /note="Alpha"
FT BINDING 5
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMF"
FT BINDING 6
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMF"
FT BINDING 6
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMF"
FT BINDING 10
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMF"
FT BINDING 10
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="3"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMF"
FT BINDING 17
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMF"
FT BINDING 17
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="3"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMF"
FT BINDING 21
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMF"
FT BINDING 23
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMF"
FT BINDING 26
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMF"
FT BINDING 26
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="3"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMF"
FT BINDING 28
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="3"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMF"
FT BINDING 31
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="4"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMC"
FT BINDING 34
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="4"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMC"
FT BINDING 34
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="5"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMC"
FT BINDING 38
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="5"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMC"
FT BINDING 40
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="6"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMC"
FT BINDING 46
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="6"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMC"
FT BINDING 50
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="4"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMC"
FT BINDING 50
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="6"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMC"
FT BINDING 54
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="4"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMC"
FT BINDING 56
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="5"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMC"
FT BINDING 57
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="5"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMC"
FT BINDING 57
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="6"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:7819257,
FT ECO:0007744|PDB:1DMC"
FT TURN 12..15
FT /evidence="ECO:0007829|PDB:1DME"
FT STRAND 24..26
FT /evidence="ECO:0007829|PDB:1DMF"
FT TURN 32..37
FT /evidence="ECO:0007829|PDB:1DMC"
FT HELIX 43..49
FT /evidence="ECO:0007829|PDB:1DMC"
FT STRAND 55..57
FT /evidence="ECO:0007829|PDB:1DMC"
SQ SEQUENCE 59 AA; 6141 MW; 4398B0A9D1A96D34 CRC64;
MPGPCCNDKC VCQEGGCKAG CQCTSCRCSP CQKCTSGCKC ATKEECSKTC TKPCSCCPK
