MT25B_HUMAN
ID MT25B_HUMAN Reviewed; 475 AA.
AC Q96FB5; D3DVC7; Q4VX71; Q5SZ03; Q9Y358;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Methyltransferase-like protein 25B {ECO:0000312|HGNC:HGNC:24273};
DE AltName: Full=Protein RRNAD1;
DE AltName: Full=Ribosomal RNA adenine dimethylase domain-containing protein 1;
GN Name=METTL25B {ECO:0000312|HGNC:HGNC:24273};
GN Synonyms=C1orf66, RRNAD1 {ECO:0000312|HGNC:HGNC:24273}; ORFNames=CGI-41;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT LEU-400.
RX PubMed=10810093; DOI=10.1101/gr.10.5.703;
RA Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.;
RT "Identification of novel human genes evolutionarily conserved in
RT Caenorhabditis elegans by comparative proteomics.";
RL Genome Res. 10:703-713(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT LEU-400.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT LEU-400.
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q96FB5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96FB5-2; Sequence=VSP_054043, VSP_054044;
CC -!- SIMILARITY: Belongs to the METTL25 family. {ECO:0000305}.
CC -!- CAUTION: Was named RRNAD1 by HGNC because a ribosomal RNA adenine
CC dimethylase signature is detected by PROSITE. However, it probably
CC constitutes a false positive and the ribosomal RNA adenine dimethylase
CC signature is not conserved in orthologs. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD34037.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF151800; AAD34037.1; ALT_FRAME; mRNA.
DR EMBL; AL590666; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471121; EAW52916.1; -; Genomic_DNA.
DR EMBL; CH471121; EAW52918.1; -; Genomic_DNA.
DR EMBL; BC011382; AAH11382.1; -; mRNA.
DR CCDS; CCDS1154.1; -. [Q96FB5-1]
DR CCDS; CCDS44246.1; -. [Q96FB5-2]
DR RefSeq; NP_001136032.1; NM_001142560.1. [Q96FB5-2]
DR RefSeq; NP_057081.3; NM_015997.3. [Q96FB5-1]
DR AlphaFoldDB; Q96FB5; -.
DR SMR; Q96FB5; -.
DR BioGRID; 119282; 3.
DR STRING; 9606.ENSP00000357199; -.
DR iPTMnet; Q96FB5; -.
DR PhosphoSitePlus; Q96FB5; -.
DR BioMuta; RRNAD1; -.
DR DMDM; 152125834; -.
DR EPD; Q96FB5; -.
DR jPOST; Q96FB5; -.
DR MassIVE; Q96FB5; -.
DR MaxQB; Q96FB5; -.
DR PaxDb; Q96FB5; -.
DR PeptideAtlas; Q96FB5; -.
DR PRIDE; Q96FB5; -.
DR ProteomicsDB; 62319; -.
DR ProteomicsDB; 76507; -. [Q96FB5-1]
DR Antibodypedia; 34224; 11 antibodies from 7 providers.
DR DNASU; 51093; -.
DR Ensembl; ENST00000368216.9; ENSP00000357199.4; ENSG00000143303.12. [Q96FB5-1]
DR Ensembl; ENST00000368218.8; ENSP00000357201.4; ENSG00000143303.12. [Q96FB5-2]
DR GeneID; 51093; -.
DR KEGG; hsa:51093; -.
DR MANE-Select; ENST00000368216.9; ENSP00000357199.4; NM_015997.4; NP_057081.3.
DR UCSC; uc001fpu.4; human. [Q96FB5-1]
DR CTD; 51093; -.
DR DisGeNET; 51093; -.
DR GeneCards; METTL25B; -.
DR HGNC; HGNC:24273; METTL25B.
DR HPA; ENSG00000143303; Low tissue specificity.
DR neXtProt; NX_Q96FB5; -.
DR OpenTargets; ENSG00000143303; -.
DR VEuPathDB; HostDB:ENSG00000143303; -.
DR eggNOG; KOG2651; Eukaryota.
DR GeneTree; ENSGT00530000063745; -.
DR HOGENOM; CLU_1004576_0_0_1; -.
DR InParanoid; Q96FB5; -.
DR OMA; IVGLHPC; -.
DR OrthoDB; 1014832at2759; -.
DR PhylomeDB; Q96FB5; -.
DR TreeFam; TF312998; -.
DR PathwayCommons; Q96FB5; -.
DR BioGRID-ORCS; 51093; 10 hits in 1076 CRISPR screens.
DR ChiTaRS; RRNAD1; human.
DR GenomeRNAi; 51093; -.
DR Pharos; Q96FB5; Tdark.
DR PRO; PR:Q96FB5; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q96FB5; protein.
DR Bgee; ENSG00000143303; Expressed in right uterine tube and 173 other tissues.
DR ExpressionAtlas; Q96FB5; baseline and differential.
DR Genevisible; Q96FB5; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000179; F:rRNA (adenine-N6,N6-)-dimethyltransferase activity; IEA:InterPro.
DR InterPro; IPR025714; Methyltranfer_dom.
DR InterPro; IPR020596; rRNA_Ade_Mease_Trfase_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF13679; Methyltransf_32; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..475
FT /note="Methyltransferase-like protein 25B"
FT /id="PRO_0000289052"
FT TRANSMEM 406..426
FT /note="Helical"
FT /evidence="ECO:0000255"
FT COILED 186..210
FT /evidence="ECO:0000255"
FT VAR_SEQ 213..277
FT /note="VVQTSPRHSPHHVVRWVDPTALCEELLLPLENPCQGRARLLLTGLHACGDLS
FT VALLRHFSCCPEV -> ICAAGATASGARSPAATESGCPSGPRGPGEPCGGLLQPGSTA
FT CPTGGDAYSTGPAAVPSGTGFPC (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_054043"
FT VAR_SEQ 278..475
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_054044"
FT VARIANT 400
FT /note="V -> L (in dbSNP:rs4311877)"
FT /evidence="ECO:0000269|PubMed:10810093,
FT ECO:0000269|PubMed:15489334, ECO:0000269|Ref.3"
FT /id="VAR_032564"
FT CONFLICT 436
FT /note="G -> L (in Ref. 4; AAH11382)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 475 AA; 52981 MW; 36B0552C8CD0582F CRC64;
MPGISARGLS HEGRKQLAVN LTRVLALYRS ILDAYIIEFF TDNLWDTLPC SWQEALDGLK
PPQLATMLLG MPGEGEVVRY RSVWPLTLLA LKSTACALAF TRMPGFQTPS EFLENPSQSS
RLTAPFRKHV RPKKQHEIRR LGELVKKLSD FTGCTQVVDV GSGQGHLSRF MALGLGLMVK
SIEGDQRLVE RAQRLDQELL QALEKEEKRN PQVVQTSPRH SPHHVVRWVD PTALCEELLL
PLENPCQGRA RLLLTGLHAC GDLSVALLRH FSCCPEVVAL ASVGCCYMKL SDPGGYPLSQ
WVAGLPGYEL PYRLREGACH ALEEYAERLQ KAGPGLRTHC YRAALETVIR RARPELRRPG
VQGIPRVHEL KIEEYVQRGL QRVGLDPQLP LNLAALQAHV AQENRVVAFF SLALLLAPLV
ETLILLDRLL YLQEQGFHAE LLPIFSPELS PRNLVLVATK MPLGQALSVL ETEDS
