MT25B_MOUSE
ID MT25B_MOUSE Reviewed; 475 AA.
AC Q8BZG5; G3X8Q8; Q8CII4;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-SEP-2021, sequence version 2.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Methyltransferase-like protein 25B;
DE AltName: Full=Protein RRNAD1 {ECO:0000250|UniProtKB:Q96FB5};
GN Name=Mettl25b {ECO:0000250|UniProtKB:Q96FB5};
GN Synonyms=Rrnad1 {ECO:0000312|MGI:MGI:2387197};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Urinary bladder;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BZG5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BZG5-2; Sequence=VSP_025878, VSP_025879, VSP_025880;
CC -!- SIMILARITY: Belongs to the METTL25 family. {ECO:0000305}.
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DR EMBL; AK035389; BAC29055.1; -; mRNA.
DR EMBL; AC158233; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC023814; AAH23814.1; -; mRNA.
DR CCDS; CCDS17458.1; -. [Q8BZG5-1]
DR RefSeq; NP_705790.3; NM_153562.4.
DR AlphaFoldDB; Q8BZG5; -.
DR STRING; 10090.ENSMUSP00000005016; -.
DR PhosphoSitePlus; Q8BZG5; -.
DR MaxQB; Q8BZG5; -.
DR PaxDb; Q8BZG5; -.
DR PRIDE; Q8BZG5; -.
DR ProteomicsDB; 299932; -. [Q8BZG5-1]
DR ProteomicsDB; 299933; -. [Q8BZG5-2]
DR ProteomicsDB; 329131; -.
DR Antibodypedia; 34224; 11 antibodies from 7 providers.
DR Ensembl; ENSMUST00000005016; ENSMUSP00000005016; ENSMUSG00000004896. [Q8BZG5-1]
DR GeneID; 229503; -.
DR KEGG; mmu:229503; -.
DR UCSC; uc008pti.2; mouse. [Q8BZG5-2]
DR CTD; 229503; -.
DR MGI; MGI:2387197; Rrnad1.
DR VEuPathDB; HostDB:ENSMUSG00000004896; -.
DR eggNOG; KOG2651; Eukaryota.
DR GeneTree; ENSGT00530000063745; -.
DR HOGENOM; CLU_016581_3_0_1; -.
DR InParanoid; Q8BZG5; -.
DR OMA; IVGLHPC; -.
DR OrthoDB; 1014832at2759; -.
DR PhylomeDB; Q8BZG5; -.
DR TreeFam; TF312998; -.
DR BioGRID-ORCS; 229503; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Rrnad1; mouse.
DR PRO; PR:Q8BZG5; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q8BZG5; protein.
DR Bgee; ENSMUSG00000004896; Expressed in retinal neural layer and 221 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR025714; Methyltranfer_dom.
DR Pfam; PF13679; Methyltransf_32; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..475
FT /note="Methyltransferase-like protein 25B"
FT /id="PRO_0000289053"
FT TRANSMEM 406..426
FT /note="Helical"
FT /evidence="ECO:0000255"
FT COILED 185..209
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..64
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025878"
FT VAR_SEQ 65..92
FT /note="ATLLLGMPRDGEEMRYRSVWPLTLLALK -> MARSTGWTEPTTAGHPAAGD
FT AEGRGGNE (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025879"
FT VAR_SEQ 375..475
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025880"
FT CONFLICT 44
FT /note="L -> P (in Ref. 1; BAC29055)"
FT CONFLICT 353
FT /note="C -> Y (in Ref. 1; BAC29055)"
SQ SEQUENCE 475 AA; 53259 MW; 0A206128EEBB36D4 CRC64;
MRGVSAHGLS HEERRQLAVD LTRVLAHYRS ILDAYIIEFF TDSLWGTLPH SWQEALDGLN
PPQLATLLLG MPRDGEEMRY RSVWPLTLLA LKSTACALAF TRTPGFHTPS EFLENPSQSS
RLTAPFRKHV KPKKQHEIRR LGELVKKLSD LTGCTQVVDV GSGQGHLSRF MSLGLGLMVK
SLEGNQRLVK RAQHLDQELL KALDKMEKRH PKMVQRGPRH RPHHVVQWVS PTTLCEELLL
PLERPGQSSA RLLLTGLHAC GDLSVALLRH FCCCPEVVAL ASVGCCYMKL SDPGSYPLSQ
WIAGLPGHEL PYRLREGACH ALEDYAERLH KAEPGLQTHC FRAALETVIR QVCPELRRPG
VQGIPRVHEL KIEEYVKQGL KRVGLDPQLP LDLAALQAHQ AQENRVVAFF SLALLLAPLV
ETLILLDRML YLQEQGFYAE LLPIFSPELS PRNLVLVATK TPLGQAFSTL ETEDN
