MT2A_ARATH
ID MT2A_ARATH Reviewed; 81 AA.
AC P25860; P43391;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Metallothionein-like protein 2A;
DE Short=MT-2A;
DE Short=MT-K;
DE AltName: Full=MT-1G;
GN Name=MT2A; OrderedLocusNames=At3g09390; ORFNames=F3L24.28;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Columbia; TISSUE=Leaf;
RA Takahashi K.;
RT "A. thaliana gene for metallothionein-like protein, complete cds.";
RL Submitted (OCT-1991) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=7612924; DOI=10.3109/10425179509029353;
RA Yeh S.C., Hsieh H.M., Huang P.C.;
RT "Transcripts of metallothionein genes in Arabidopsis thaliana.";
RL DNA Seq. 5:141-144(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=7565594; DOI=10.1007/bf02191599;
RA Zhou J., Goldsbrough P.B.;
RT "Structure, organization and expression of the metallothionein gene family
RT in Arabidopsis.";
RL Mol. Gen. Genet. 248:318-328(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 17-81.
RC STRAIN=cv. Columbia;
RX PubMed=8580968; DOI=10.1046/j.1365-313x.1996.09010101.x;
RA Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C.,
RA Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R.,
RA Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J.,
RA Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D.,
RA Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P.,
RA Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H.;
RT "Further progress towards a catalogue of all Arabidopsis genes: analysis of
RT a set of 5000 non-redundant ESTs.";
RL Plant J. 9:101-124(1996).
RN [8]
RP TISSUE SPECIFICITY.
RX DOI=10.1046/j.1469-8137.2003.00813.x;
RA Guo W.J., Bundithya W., Goldsbrough P.B.;
RT "Characterization of the Arabidopsis metallothionein gene family: tissue-
RT specific expression and induction during senescence and in response to
RT copper.";
RL New Phytol. 159:369-381(2003).
RN [9]
RP FUNCTION.
RX PubMed=18287486; DOI=10.1104/pp.108.115782;
RA Guo W.J., Meetam M., Goldsbrough P.B.;
RT "Examining the specific contributions of individual Arabidopsis
RT metallothioneins to copper distribution and metal tolerance.";
RL Plant Physiol. 146:1697-1706(2008).
RN [10]
RP FUNCTION.
RX PubMed=24635746; DOI=10.1111/nph.12718;
RA Benatti M.R., Yookongkaew N., Meetam M., Guo W.J., Punyasuk N.,
RA Abuqamar S., Goldsbrough P.;
RT "Metallothionein deficiency impacts copper accumulation and redistribution
RT in leaves and seeds of Arabidopsis.";
RL New Phytol. 202:940-951(2014).
CC -!- FUNCTION: Metallothioneins have a high content of cysteine residues
CC that bind various heavy metals (Probable). Functions as metal chelator
CC of copper (Cu) and zinc (Zn) (PubMed:18287486). Plays a role in Cu
CC homeostasis, specifically in the remobilization of Cu from senescing
CC leaves. The mobilization of Cu from internal sources is important for
CC seed development (PubMed:24635746). {ECO:0000269|PubMed:18287486,
CC ECO:0000269|PubMed:24635746, ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Abundant in leaves, inflorescences and roots of
CC mature plants. Also detected in roots of young plants, and in siliques
CC (PubMed:7565594). Expressed in leaf mesophyll cells, filaments, stigma
CC and tips of elongating lateral roots (Ref.8).
CC {ECO:0000269|PubMed:7565594, ECO:0000269|Ref.8}.
CC -!- INDUCTION: Strongly induced in seedlings by CuSO4.
CC {ECO:0000269|PubMed:7565594}.
CC -!- SIMILARITY: Belongs to the metallothionein superfamily. Type 15 family.
CC {ECO:0000305}.
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DR EMBL; D11394; BAA01990.1; -; Genomic_DNA.
DR EMBL; X62818; CAA44630.1; -; mRNA.
DR EMBL; U15108; AAA50250.1; -; mRNA.
DR EMBL; AC011436; AAF14034.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74760.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM65194.1; -; Genomic_DNA.
DR EMBL; AY037263; AAK59864.1; -; mRNA.
DR EMBL; AY077669; AAL76147.1; -; mRNA.
DR EMBL; Z26416; CAA81248.1; -; mRNA.
DR PIR; S18069; SMMUL.
DR PIR; S57861; S57861.
DR RefSeq; NP_001327181.1; NM_001337815.1.
DR RefSeq; NP_187550.1; NM_111773.4.
DR AlphaFoldDB; P25860; -.
DR BioGRID; 5432; 4.
DR STRING; 3702.AT3G09390.1; -.
DR PaxDb; P25860; -.
DR PRIDE; P25860; -.
DR ProteomicsDB; 250897; -.
DR EnsemblPlants; AT3G09390.1; AT3G09390.1; AT3G09390.
DR EnsemblPlants; AT3G09390.2; AT3G09390.2; AT3G09390.
DR GeneID; 820098; -.
DR Gramene; AT3G09390.1; AT3G09390.1; AT3G09390.
DR Gramene; AT3G09390.2; AT3G09390.2; AT3G09390.
DR KEGG; ath:AT3G09390; -.
DR Araport; AT3G09390; -.
DR TAIR; locus:2083519; AT3G09390.
DR eggNOG; KOG4738; Eukaryota.
DR HOGENOM; CLU_161105_1_0_1; -.
DR InParanoid; P25860; -.
DR OMA; EADHGCS; -.
DR OrthoDB; 1627904at2759; -.
DR PhylomeDB; P25860; -.
DR PRO; PR:P25860; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; P25860; baseline and differential.
DR Genevisible; P25860; AT.
DR GO; GO:0005507; F:copper ion binding; IDA:TAIR.
DR GO; GO:0006878; P:cellular copper ion homeostasis; ISS:TAIR.
DR InterPro; IPR000347; Metalthion_15p.
DR PANTHER; PTHR33543; PTHR33543; 1.
DR Pfam; PF01439; Metallothio_2; 1.
PE 2: Evidence at transcript level;
KW Metal-binding; Metal-thiolate cluster; Reference proteome.
FT CHAIN 1..81
FT /note="Metallothionein-like protein 2A"
FT /id="PRO_0000197386"
FT CONFLICT 64
FT /note="N -> S (in Ref. 1; BAA01990/CAA44630)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 81 AA; 8163 MW; B2639167E56854EB CRC64;
MSCCGGNCGC GSGCKCGNGC GGCKMYPDLG FSGETTTTET FVLGVAPAMK NQYEASGESN
NAENDACKCG SDCKCDPCTC K
