ID   MT2B_COLES              Reviewed;          84 AA.
AC   Q19LA2;
DT   06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Metallothionein type 2b;
DE            Short=CeMT2b;
OS   Colocasia esculenta (Wild taro) (Arum esculentum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Araceae; Aroideae; Colocasieae;
OC   Colocasia.
OX   NCBI_TaxID=4460;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND MUTAGENESIS OF 82-CYS--CYS-84.
RX   PubMed=21979068; DOI=10.1271/bbb.110289;
RA   Kim Y.O., Patel D.H., Lee D.S., Song Y., Bae H.J.;
RT   "High cadmium-binding ability of a novel Colocasia esculenta
RT   metallothionein increases cadmium tolerance in Escherichia coli and
RT   Tobacco.";
RL   Biosci. Biotechnol. Biochem. 75:1912-1920(2011).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND MUTAGENESIS.
RX   PubMed=22610130; DOI=10.1007/s10265-012-0492-8;
RA   Kim Y.O., Lee Y.G., Patel D.H., Kim H.M., Ahn S.J., Bae H.J.;
RT   "Zn tolerance of novel Colocasia esculenta metallothionein and its domains
RT   in Escherichia coli and tobacco.";
RL   J. Plant Res. 125:793-804(2012).
RN   [3]
RP   FUNCTION, AND INDUCTION.
RA   Parmar P., Patel M., Dave B., Subramanian R.B., Bae H.J.;
RT   "Isolation, cloning and expression of novel metallothionine type II protein
RT   from Colocassia esculentum.";
RL   Univers. J. Med. Dent. 1:37-45(2012).
CC   -!- FUNCTION: Metallothioneins have a high content of cysteine residues
CC       that bind various heavy metals. Probably involved in maintaining
CC       homeostasis of essential transition metals and detoxification of toxic
CC       metals. Increases cadmium and zinc tolerance when expressed in
CC       heterologous systems. Metal chelator binding 6 cadmium or 5 zinc atoms
CC       per protein. {ECO:0000269|PubMed:21979068, ECO:0000269|PubMed:22610130,
CC       ECO:0000269|Ref.3}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22610130}. Nucleus
CC       {ECO:0000269|PubMed:22610130}.
CC   -!- TISSUE SPECIFICITY: Expressed in leaves, stems and roots.
CC       {ECO:0000269|PubMed:22610130}.
CC   -!- INDUCTION: Up-regulated by cadmium treatment. {ECO:0000269|Ref.3}.
CC   -!- DOMAIN: The N-terminal (1-23) and C-terminal (66-84) Cys-rich domains
CC       are both involved in zinc binding.
CC   -!- SIMILARITY: Belongs to the metallothionein superfamily. Type 15 family.
CC       {ECO:0000305}.
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DR   EMBL; DQ521570; ABF71737.1; -; mRNA.
DR   AlphaFoldDB; Q19LA2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR000347; Metalthion_15p.
DR   PANTHER; PTHR33543; PTHR33543; 1.
DR   Pfam; PF01439; Metallothio_2; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Metal-binding; Metal-thiolate cluster; Nucleus.
FT   CHAIN           1..84
FT                   /note="Metallothionein type 2b"
FT                   /id="PRO_0000421160"
FT   MUTAGEN         82..84
FT                   /note="Missing: Decreased protein stability and cadmium
FT                   binding."
FT                   /evidence="ECO:0000269|PubMed:21979068"
SQ   SEQUENCE   84 AA;  8414 MW;  5685ABAF658179E0 CRC64;
     MSCCGGNCGC GSGCQCGNGC GGCKMFPDFG SDEKITTTHT LVLGFAPAKG SVEGFEMVAG
     AAENDCKCGS NCSCTDCRCD PCNC