ID   MT2C_ORYSJ              Reviewed;          84 AA.
AC   A3AZ88; P93433; Q0DLB2; Q65XV5;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Metallothionein-like protein 2C;
DE   AltName: Full=Class I metallothionein-like protein 2C;
DE   AltName: Full=OsMT-I-2c;
DE   AltName: Full=OsMT2b;
GN   Name=MT2C; Synonyms=MTE; OrderedLocusNames=Os05g0111300, LOC_Os05g02070;
GN   ORFNames=OsJ_016110, P0016H04.13;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=cv. Lomello;
RA   Chen P.-W., Chen L.-J.;
RT   "Isolation of a cDNA clone encoding a type 2 metallothionein-like protein
RT   from rice embryo.";
RL   (er) Plant Gene Register PGR97-109(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA   Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA   Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA   Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT   "A fine physical map of the rice chromosome 5.";
RL   Mol. Genet. Genomics 274:337-345(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [7]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RX   PubMed=15220467; DOI=10.1104/pp.103.036384;
RA   Wong H.L., Sakamoto T., Kawasaki T., Umemura K., Shimamoto K.;
RT   "Down-regulation of metallothionein, a reactive oxygen scavenger, by the
RT   small GTPase OsRac1 in rice.";
RL   Plant Physiol. 135:1447-1456(2004).
RN   [8]
RP   GENE FAMILY, AND TISSUE SPECIFICITY.
RX   PubMed=17002881; DOI=10.5483/bmbrep.2006.39.5.595;
RA   Zhou G., Xu Y., Li J., Yang L., Liu J.-Y.;
RT   "Molecular analyses of the metallothionein gene family in rice (Oryza
RT   sativa L.).";
RL   J. Biochem. Mol. Biol. 39:595-606(2006).
RN   [9]
RP   FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX   PubMed=18258694; DOI=10.1104/pp.107.110304;
RA   Yuan J., Chen D., Ren Y., Zhang X., Zhao J.;
RT   "Characteristic and expression analysis of a metallothionein gene, OsMT2b,
RT   down-regulated by cytokinin suggests functions in root development and seed
RT   embryo germination of rice.";
RL   Plant Physiol. 146:1637-1650(2008).
CC   -!- FUNCTION: Metallothioneins have a high content of cysteine residues
CC       that bind various heavy metals (Probable). Acts as reactive oxygen
CC       species (ROS) scavenger in the cytosol. Possesses superoxide anion and
CC       hydroxyl radical scavenging activities in vitro (PubMed:15220467).
CC       Plays a role during root development, lateral root initiation and seed
CC       embryo germination, possibly by regulating levels of cytokinin
CC       (PubMed:18258694). {ECO:0000269|PubMed:15220467,
CC       ECO:0000269|PubMed:18258694, ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:15220467}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in inflorescences. Expressed in
CC       roots and leaves (PubMed:15220467, PubMed:17002881). Expressed in root
CC       primordium, pericycle, phloem and in the basal parts of lateral roots
CC       (PubMed:18258694). {ECO:0000269|PubMed:15220467,
CC       ECO:0000269|PubMed:17002881, ECO:0000269|PubMed:18258694}.
CC   -!- DEVELOPMENTAL STAGE: During embryo development, expressed in the top of
CC       the scutellum at 7 days after pollination (DAP). In the germinating
CC       embryos, expressed in the scutellum and the cap of the radicle at 2
CC       days after germination (DAG). At 4 DAG, still expressed in scutellum,
CC       and appears in the vascular bundle of shoot. In florets of young
CC       panicle, expressed in the pedicel and the basement of glumes. In
CC       florets of older panicle, expressed in the pedicel, basement of glumes,
CC       anthers and pistils. Expressed in the basal parts of ovaries before
CC       pollination and in the basal parts of stigmas and ovaries after
CC       pollination. {ECO:0000269|PubMed:18258694}.
CC   -!- INDUCTION: By Fe, Zn and auxin in roots, and Cu and Mn in shoots. Down-
CC       regulated by cytokinin and cold stress in roots and shoots. Down-
CC       regulated by Cu and salt stress in roots (PubMed:18258694).
CC       Sinergically down-regulated by RAC1 and sphingolipid elicitor
CC       (PubMed:15220467). {ECO:0000269|PubMed:15220467,
CC       ECO:0000269|PubMed:18258694}.
CC   -!- MISCELLANEOUS: Down-regulation by RAC1 during pathogen infection may
CC       potentiate ROS which function as signals in resistance response
CC       (PubMed:15220467). Plants silencing MT2C show abnormal plant growth and
CC       root formation (PubMed:18258694). {ECO:0000269|PubMed:15220467,
CC       ECO:0000269|PubMed:18258694}.
CC   -!- SIMILARITY: Belongs to the metallothionein superfamily. Type 15 family.
CC       {ECO:0000305}.
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DR   EMBL; U57638; AAD10192.1; -; mRNA.
DR   EMBL; AF048750; AAD02475.1; -; Genomic_DNA.
DR   EMBL; AC079356; AAU44207.1; -; Genomic_DNA.
DR   EMBL; AP008211; BAF16361.1; -; Genomic_DNA.
DR   EMBL; AP014961; BAS91926.1; -; Genomic_DNA.
DR   EMBL; CM000142; EAZ32627.1; -; Genomic_DNA.
DR   RefSeq; XP_015638726.1; XM_015783240.1.
DR   AlphaFoldDB; A3AZ88; -.
DR   STRING; 4530.OS05T0111300-01; -.
DR   PRIDE; A3AZ88; -.
DR   EnsemblPlants; Os05t0111300-01; Os05t0111300-01; Os05g0111300.
DR   GeneID; 4337596; -.
DR   Gramene; Os05t0111300-01; Os05t0111300-01; Os05g0111300.
DR   KEGG; osa:4337596; -.
DR   eggNOG; ENOG502T0EQ; Eukaryota.
DR   HOGENOM; CLU_161105_1_0_1; -.
DR   InParanoid; A3AZ88; -.
DR   OMA; TCTAVCA; -.
DR   OrthoDB; 1648303at2759; -.
DR   Proteomes; UP000000763; Chromosome 5.
DR   Proteomes; UP000007752; Chromosome 5.
DR   Proteomes; UP000059680; Chromosome 5.
DR   ExpressionAtlas; A3AZ88; baseline and differential.
DR   Genevisible; A3AZ88; OS.
DR   GO; GO:0005829; C:cytosol; IMP:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0072593; P:reactive oxygen species metabolic process; IMP:UniProtKB.
DR   InterPro; IPR000347; Metalthion_15p.
DR   PANTHER; PTHR33543; PTHR33543; 1.
DR   Pfam; PF01439; Metallothio_2; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Metal-binding; Metal-thiolate cluster; Reference proteome;
KW   Stress response.
FT   CHAIN           1..84
FT                   /note="Metallothionein-like protein 2C"
FT                   /id="PRO_0000197408"
SQ   SEQUENCE   84 AA;  7969 MW;  5BF71575A9142311 CRC64;
     MSCCGGNCGC GSGCQCGGGC GGCKMFPDVE ATATTKTFVL AAPSNKASSG GMEMAVESGE
     NGGCGCNTCK CGTSCSGCSC CSCN