MT2_RAT
ID MT2_RAT Reviewed; 61 AA.
AC P04355;
DT 20-MAR-1987, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-1987, sequence version 1.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Metallothionein-2;
DE Short=MT-2;
DE AltName: Full=Metallothionein-II;
DE Short=MT-II;
GN Name=Mt2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP PROTEIN SEQUENCE, AND ACETYLATION AT MET-1.
RC TISSUE=Liver;
RX PubMed=6470004; DOI=10.1016/s0021-9258(18)90877-x;
RA Winge D.R., Nielson K.B., Zeikus R.D., Gray W.R.;
RT "Structural characterization of the isoforms of neonatal and adult rat
RT liver metallothionein.";
RL J. Biol. Chem. 259:11419-11425(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2959527; DOI=10.1007/978-3-0348-6784-9_35;
RA Andersen R.D., Taplitz S.J., Birren B.W., Bristol G., Herschman H.R.;
RT "Rat metallothionein multigene family.";
RL Experientia Suppl. 52:373-384(1987).
RN [3]
RP CRYSTALLIZATION.
RX PubMed=6853483; DOI=10.1016/s0021-9258(18)32400-1;
RA Melis K.A., Carter D.C., Stout C.D., Winge D.R.;
RT "Single crystals of cadmium, zinc metallothionein.";
RL J. Biol. Chem. 258:6255-6257(1983).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
RX PubMed=3945804; DOI=10.1126/science.3945804;
RA Furey W.F. Jr., Robbins A.H., Clancy L.L., Winge D.R., Wand B.C.,
RA Stout C.D.;
RT "Crystal structure of Cd,Zn metallothionein.";
RL Science 231:704-710(1986).
RN [5]
RP STRUCTURE BY NMR.
RX PubMed=3653102; DOI=10.1111/j.1432-1033.1987.tb13359.x;
RA Woergoetter E., Wagner G., Vasak M., Kaegi J.H.R., Wuethrich K.;
RT "Sequence-specific 1H-NMR assignments in rat-liver metallothionein-2.";
RL Eur. J. Biochem. 167:457-466(1987).
RN [6] {ECO:0007744|PDB:1MRT, ECO:0007744|PDB:2MRT}
RP STRUCTURE BY NMR IN COMPLEX WITH CADMIUM IONS, AND DOMAIN.
RX PubMed=3184190; DOI=10.1016/0022-2836(88)90106-4;
RA Schultze P., Woergotter E., Braun W., Wagner G., Vasak M., Kaegi J.H.R.,
RA Wuethrich K.;
RT "Conformation of [Cd7]-metallothionein-2 from rat liver in aqueous solution
RT determined by nuclear magnetic resonance spectroscopy.";
RL J. Mol. Biol. 203:251-268(1988).
RN [7] {ECO:0007744|PDB:4MT2}
RP X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH ZINC AND CADMIUM
RP IONS, AND DOMAIN.
RX PubMed=1942051; DOI=10.1016/0022-2836(91)90933-w;
RA Robbins A.H., McRee D.E., Williamson M., Collett S.A., Xuong N.H.,
RA Furey W.F. Jr., Wang B.C., Stout C.D.;
RT "Refined crystal structure of Cd, Zn metallothionein at 2.0-A resolution.";
RL J. Mol. Biol. 221:1269-1293(1991).
CC -!- FUNCTION: Metallothioneins have a high content of cysteine residues
CC that bind various heavy metals; these proteins are transcriptionally
CC regulated by both heavy metals and glucocorticoids.
CC -!- DOMAIN: Class I metallothioneins contain 2 metal-binding domains: four
CC divalent ions are chelated within cluster A of the alpha domain and are
CC coordinated via cysteinyl thiolate bridges to 11 cysteine ligands.
CC Cluster B, the corresponding region within the beta domain, can ligate
CC three divalent ions to 9 cysteines. {ECO:0000269|PubMed:1942051,
CC ECO:0000269|PubMed:3184190}.
CC -!- SIMILARITY: Belongs to the metallothionein superfamily. Type 1 family.
CC {ECO:0000305}.
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DR EMBL; M11794; AAA41640.1; -; Genomic_DNA.
DR PIR; B61561; SMRT2.
DR RefSeq; NP_001131036.1; NM_001137564.1.
DR PDB; 1MRT; NMR; -; A=31-61.
DR PDB; 2MRT; NMR; -; A=1-30.
DR PDB; 4MT2; X-ray; 2.00 A; A=1-61.
DR PDBsum; 1MRT; -.
DR PDBsum; 2MRT; -.
DR PDBsum; 4MT2; -.
DR AlphaFoldDB; P04355; -.
DR BMRB; P04355; -.
DR SMR; P04355; -.
DR IntAct; P04355; 1.
DR STRING; 10116.ENSRNOP00000062585; -.
DR iPTMnet; P04355; -.
DR PaxDb; P04355; -.
DR Ensembl; ENSRNOT00000067391; ENSRNOP00000062585; ENSRNOG00000043098.
DR GeneID; 689415; -.
DR KEGG; rno:689415; -.
DR UCSC; RGD:1592345; rat.
DR CTD; 4502; -.
DR eggNOG; KOG4738; Eukaryota.
DR GeneTree; ENSGT00950000182967; -.
DR HOGENOM; CLU_171204_2_0_1; -.
DR InParanoid; P04355; -.
DR OMA; CKECKCA; -.
DR PhylomeDB; P04355; -.
DR TreeFam; TF336054; -.
DR Reactome; R-RNO-5661231; Metallothioneins bind metals.
DR EvolutionaryTrace; P04355; -.
DR PRO; PR:P04355; -.
DR Proteomes; UP000002494; Chromosome 19.
DR Bgee; ENSRNOG00000043098; Expressed in kidney and 20 other tissues.
DR ExpressionAtlas; P04355; baseline and differential.
DR Genevisible; P04355; RN.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; ISS:UniProtKB.
DR GO; GO:0071276; P:cellular response to cadmium ion; IBA:GO_Central.
DR GO; GO:0071280; P:cellular response to copper ion; IBA:GO_Central.
DR GO; GO:0071294; P:cellular response to zinc ion; ISS:UniProtKB.
DR GO; GO:0006882; P:cellular zinc ion homeostasis; IBA:GO_Central.
DR GO; GO:0010273; P:detoxification of copper ion; IBA:GO_Central.
DR GO; GO:0045926; P:negative regulation of growth; ISS:UniProtKB.
DR Gene3D; 4.10.10.10; -; 1.
DR InterPro; IPR003019; Metalthion.
DR InterPro; IPR017854; Metalthion_dom_sf.
DR InterPro; IPR023587; Metalthion_dom_sf_vert.
DR InterPro; IPR000006; Metalthion_vert.
DR InterPro; IPR018064; Metalthion_vert_metal_BS.
DR PANTHER; PTHR23299; PTHR23299; 1.
DR Pfam; PF00131; Metallothio; 1.
DR PRINTS; PR00860; MTVERTEBRATE.
DR SUPFAM; SSF57868; SSF57868; 1.
DR PROSITE; PS00203; METALLOTHIONEIN_VRT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Cadmium; Direct protein sequencing;
KW Metal-binding; Metal-thiolate cluster; Phosphoprotein; Reference proteome;
KW Zinc.
FT CHAIN 1..61
FT /note="Metallothionein-2"
FT /id="PRO_0000197222"
FT REGION 1..29
FT /note="Beta"
FT REGION 30..61
FT /note="Alpha"
FT BINDING 5
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:2MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 7
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:2MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 7
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:2MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 13
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:2MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 15
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:2MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 15
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="3"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:2MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 19
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="3"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:2MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 21
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:2MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 24
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:2MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 24
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="3"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:2MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 26
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:2MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 29
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="3"
FT /ligand_note="in cluster B"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:2MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 33
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="4"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:1MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 34
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="4"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:1MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 34
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="5"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:1MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 36
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="5"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:1MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 37
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="5"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:1MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 37
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="6"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:1MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 41
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="6"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:1MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 44
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="4"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:1MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 44
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="6"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:1MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 48
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="4"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:1MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 50
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="5"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:1MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 50
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="7"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:1MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 57
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="7"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:1MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 59
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="7"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:1MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 60
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="6"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:1MRT,
FT ECO:0007744|PDB:4MT2"
FT BINDING 60
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="7"
FT /ligand_note="in cluster A"
FT /evidence="ECO:0000269|PubMed:1942051,
FT ECO:0000269|PubMed:3184190, ECO:0007744|PDB:1MRT,
FT ECO:0007744|PDB:4MT2"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000269|PubMed:6470004"
FT MOD_RES 58
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P02795"
FT STRAND 9..11
FT /evidence="ECO:0007829|PDB:4MT2"
FT STRAND 16..18
FT /evidence="ECO:0007829|PDB:2MRT"
FT STRAND 35..37
FT /evidence="ECO:0007829|PDB:1MRT"
FT TURN 38..40
FT /evidence="ECO:0007829|PDB:1MRT"
FT TURN 42..46
FT /evidence="ECO:0007829|PDB:4MT2"
SQ SEQUENCE 61 AA; 6145 MW; D50D1306A5ED08E9 CRC64;
MDPNCSCATD GSCSCAGSCK CKQCKCTSCK KSCCSCCPVG CAKCSQGCIC KEASDKCSCC
A
